MicroRNA Tissue Atlas of the Malaria Mosquito <i>Anopheles gambiae</i>

Lampe, Lena; Levashina, Elena A.

doi:10.1534/g3.117.300170

Cited by 22 publications

(42 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same signals induce expression of negative regulators, including miR-276, that warrant the timely reset of the fat body metabolism (Bryant et al, 2011;Lampe and Levashina, 2018;Mane-Padros et al, 2012b). Massive degradation of AAs in the fat body supports high energy demands of nutrient mobilization and protein synthesis for successful egg development (Fuchs et al, 2014b;Zhou et al, 2004a).…”

Section: Discussionmentioning

confidence: 99%

“…However, how the miRNAs that regulate mosquito metabolism impact vector-parasite interactions is unknown. In A. gambiae, a major malaria vector in sub-Saharan Africa, blood feeding increases the fat body levels of miR-276-5p transcripts (miR-276 hereafter) during the mid-/late-phase of the reproductive cycle (Lampe and Levashina, 2018). Here, we report that miR-276 tunes the duration of the reproductive cycle by inhibiting expression of the branched chain amino acid transferase in the fat body.…”

Section: Introductionmentioning

confidence: 89%

See 1 more Smart Citation

Metabolic balancing by miR-276 shapes the mosquito reproductive cycle and Plasmodium falciparum development

Lampe

Jentzsch

Levashina

2019

Preprint

Self Cite

View full text Add to dashboard Cite

words)Anopheles mosquitoes are obligate vectors of the human malaria parasite Plasmodium falciparum. The blood-feeding behavior of Anopheles females delivers essential nutrients for egg development and drives transmission from one human host to another. Plasmodium growth is adapted to the vector reproductive cycle, but how changes in the reproductive cycle impact parasite development is poorly understood.Here, we show that the blood meal-induced miR-276-5p fine-tunes the duration of the mosquito reproductive cycle. Silencing of miR-276 prolonged amino acid catabolism and increased female fertility rates, suggesting that timely termination of the reproductive cycle restricts mosquito investment into reproduction. Prolongation of the reproductive period in P. falciparum-infected females compromised the development of the transmissible parasite form called sporozoite. Our results suggest that Plasmodium sporogony exploits surplus resources after mosquito reproductive investment and demonstrate the crucial role of the mosquito amino acid metabolism in parasite within-vector proliferation and malaria transmission.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

Metabolic balancing by miR-276 shapes the mosquito reproductive cycle and Plasmodium falciparum development

Lampe

Jentzsch

Levashina

2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…quinquefasciatus (Skalsky et al, 2010). Studies in Anopheles gambiae mosquitoes identified miR-10, miR-184, miR-281 and bantam as the most abundantly expressed miRNAs in adult females (Lampe and Levashina, 2018). In An.…”

Section: Aedes Aegypti Corpora Allata-corpora Cardiaca Mirna Repertoirementioning

confidence: 99%

“…In An. gambiae, the group of most abundant miRNAs changed before and after blood-feeding, and some of them dominated the repertoire only in specific tissues; for instance miR-281 in the midgut, miR-10 and miR-306 in the ovary and miR-8 and miR-276 in the head and fat body (Lampe and Levashina, 2018).…”

Section: Aedes Aegypti Corpora Allata-corpora Cardiaca Mirna Repertoirementioning

confidence: 99%

A comparative analysis of corpora allata-corpora cardiaca microRNA repertoires revealed significant changes during mosquito metamorphosis

Nouzová

Etebari

Noriega

et al. 2018

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

The corpora allata (CA) are a pair of endocrine glands with neural connections to the brain and close association with another neuroendocrine organ, the corpora cardiaca (CC). The CA from adult female Aedes aegypti mosquitoes synthesize fluctuating levels of juvenile hormone (JH), which have been linked to the ovarian development and are influenced by nutritional signals. In this study, we investigated the potential involvement of microRNAs (miRNAs), a type of small non-coding RNAs, in the regulation of gene expression in CA-CC complexes during mosquito reproductive development, at stages with distinct JH biosynthesis patterns. We analyzed the miRNA repertoires expressed in the CA-CC of pupae, sugar-fed and blood-fed female Ae. aegypti. In total, 156 mature miRNAs were detected in the CA-CC, with 84 displaying significant differences in expression among the three CA-CC developmental stages. There were more miRNAs that were expressed in pupae, and decreased or were absent after adult emergence, when compared with changes between CA-CC of sugar and blood-fed females. Analysis of the genes identified as potential targets for the CA-CC miRNA repertoires classified them into the broad categories of metabolism, information storage and processing, and cellular processes and signaling; with genes involved in cellular processes and signaling representing the largest portion. Among them, the signal-transduction mechanisms and intracellular trafficking, secretion and vesicular transport contained almost 55% of the genes' targets. A substantial number of miRNAs were differentially abundant in the libraries of the three developmental stages, and those changes were much more notable when pupae and adult stages were compared. We detected putative binding sites for some of the most abundant miRNAs on genes encoding JH biosynthetic enzymes and CC neuropeptides. These studies should help us to gain a better understanding of the regulation of CA-CC activity mediated by miRNAs during major developmental stages in mosquitoes.

show abstract

“…MicroRNAs (miRNAs) are small endogenous noncoding RNAs that regulate gene expression, primarily via RNA decay or posttranslational inhibition [8]. Most mosquito miRNA studies have concentrated on identification and annotation using high-throughput sequencing and subsequent bioinformatics analysis [9][10][11][12][13][14]. However, only a few miRNAs have been functionally studied in malaria mosquitoes [15,16] partly because of the lack of genome-wide resources for assessing loss of miRNA function.…”

Section: Introductionmentioning

confidence: 99%

Broad spectrum immunomodulatory effects of Anopheles gambiae microRNAs and their use for transgenic suppression of Plasmodium

Dong

et al. 2020

PLoS Pathog

View full text Add to dashboard Cite

Malaria, caused by the protozoan parasite Plasmodium and transmitted by Anopheles mosquitoes, represents a major threat to human health. Plasmodium's infection cycle in the Anopheles vector is critical for transmission of the parasite between humans. The midgutstage bottleneck of infection is largely imposed by the mosquito's innate immune system. microRNAs (miRNAs, small noncoding RNAs that bind to target RNAs to regulate gene expression) are also involved in regulating immunity and the anti-Plasmodium defense in mosquitoes. Here, we characterized the mosquito's miRNA responses to Plasmodium infection using an improved crosslinking and immunoprecipitation (CLIP) method, termed covalent ligation of endogenous Argonaute-bound RNAs (CLEAR)-CLIP. Three candidate miRNAs' influence on P. falciparum infection and midgut microbiota was studied through transgenically expressed miRNA sponges (miR-SPs) in midgut and fat body tissues. MiR-SPs mediated conditional depletion of aga-miR-14 or aga-miR-305, but not aga-miR-8, increased mosquito resistance to both P. falciparum and P. berghei infection, and enhanced the mosquitoes' antibacterial defenses. Transcriptome analysis revealed that depletion of aga-miR-14 or aga-miR-305 resulted in an increased expression of multiple immunityrelated and anti-Plasmodium genes in mosquito midguts. The overall fitness cost of conditionally expressed miR-SPs was low, with only one of eight fitness parameters being adversely affected. Taken together, our results demonstrate that targeting mosquito miRNA by conditional expression of miR-SPs may have potential for the development of malaria control through genetically engineered mosquitoes.

show abstract

MicroRNA Tissue Atlas of the Malaria Mosquito Anopheles gambiae

Cited by 22 publications

References 67 publications

Metabolic balancing by miR-276 shapes the mosquito reproductive cycle and Plasmodium falciparum development

Metabolic balancing by miR-276 shapes the mosquito reproductive cycle and Plasmodium falciparum development

A comparative analysis of corpora allata-corpora cardiaca microRNA repertoires revealed significant changes during mosquito metamorphosis

Broad spectrum immunomodulatory effects of Anopheles gambiae microRNAs and their use for transgenic suppression of Plasmodium

Contact Info

Product

Resources

About