microRNA sponging by a new hearty circRNA

Ebbesen, Karoline K; Kjems, Jørgen

doi:10.21037/ncri.2017.08.06

Cited by 4 publications

(5 citation statements)

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“…Recently, increasing studies have shown that circRNAs play critical roles in regulating gene expression by sequestering the miRNAs [23]. In the present study, we investigated whether circPCNXL2 could regulate miRNA expression in RCC progression.…”

Section: Discussionmentioning

confidence: 90%

CircPCNXL2 sponges miR-153 to promote the proliferation and invasion of renal cancer cells through upregulating ZEB2

Zhou

Zheng

et al. 2018

Cell Cycle

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Increasing evidence showed that circular RNAs (circRNAs) play critical roles in tumorigenesis. However, the roles and underlying mechanisms of circRNAs in clear cell renal cell carcinoma (ccRCC) remain unclear. In the present study, we identified a novel circRNA circPCNXL2, which was significantly upregulated in ccRCC by circular RNA microarray. Further analysis revealed that circPCNXL2 was significantly increased and correlated with poor overall survival of ccRCC patients. Function assays revealed that circPCNXL2 knockdown reduced RCC cells proliferation, invasion in vitro, and decreased tumor growth in vivo. In mechanism study, we showed that circPCNXL2 could be bind to miR-153 as a miRNA sponge to regulate ZEB2 expression in RCC progression. In addition, our data reported that the effects of circPCNXL2 inhibition on RCC cells proliferation and invasion could be abolished by miR-153 inhibitors. Altogether, we demonstrated that circPCNXL2 could regulate RCC cells proliferation and invasion by miR-153/ZEB2 axis, suggesting circPCNXL2 might serve as a potential therapeutic target for ccRCC treatment.

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Section: Discussionmentioning

confidence: 90%

CircPCNXL2 sponges miR-153 to promote the proliferation and invasion of renal cancer cells through upregulating ZEB2

Zhou

Zheng

et al. 2018

Cell Cycle

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“…RT-qPCR analysis of the probe pull-down samples showed that gga-miR-375 was enriched by 57.63 and 55.3-fold in the positive probe compared to that in the negative probe, U6 and 5S were used as the housekeeping gene ( Figure 2(i, j)). [8,14] and [9] represent ALV-J-susceptible chickens [3,4]; and [26] CircRNAs tend to accumulate in the cytoplasm of some cells and miRNAs are compartmentalized into the nucleus and cytoplasm, respectively [42,46]. We performed RNA-FISH in DF-1 cells by co-incubation with biotin-labeled circ-Vav3 and digoxin-labeled gga-miR-375 probes to determine their subcellular localization.…”

Section: Circ-vav3 Sponges With Mirna-375mentioning

confidence: 99%

“…Recent studies have shown that circRNAs may relate to Alzheimer disease, prion disease and cancer [5][6][7], and there is accumulated evidence indicating that circRNAs can be associated with cancer-related miRNAs as a regulator of cancer-related pathways and can serve as diagnostic or predictive biomarkers of some cancers [8]. Although circRNAs are intensively studied, only a relatively small number of circRNAs have been assigned a function [9]. As such, it is extremely worthwhile to further explore the novel functional circRNAs in nature.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition

et al. 2019

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Circular RNAs (circRNAs) are evolutionarily conserved and widely present, but their functions remain largely unknown. Recent development has highlighted the importance of circRNAs as the sponge of microRNA (miRNA) in cancer. We previously reported that gga-miR-375 was downregulated in the liver tumors of chickens infected with avian leukosis virus subgroup J (ALV-J) by microRNA microarray assay. It can be reasonably assumed in accordance with previous studies that the gga-miR-375 may be related to circRNAs. However, the question as to which circRNA acts as the sponge for gga-miR-375 remains to be answered. In this study, circRNA sequencing results revealed that a circRNA Vav3 termed circ-Vav3 was upregulated in the liver tumors of chickens infected with ALV-J. In addition, RNA immunoprecipitation (RIP), biotinylated RNA pull-down and RNA-fluorescence in situ hybridization (RNA-FISH) experiments were conducted to confirm that circ-Vav3 serves as the sponge of gga-miR-375. Furthermore, we confirmed through dual luciferase reporter assay that YAP1 is the target gene of gga-miR-375. The effect of the sponge function of circ-Vav3 on its downstream genes has been further verified by our conclusion that the sponge function of circ-Vav3 can abrogate gga-miR-375 target gene YAP1 and increase the expression level of YAP1. We further confirmed that the circ-Vav3/gga-miR-375/YAP1 axis induces epithelial-mesenchymal transition (EMT) through influencing EMT markers to promote tumorigenesis. Finally, clinical ALV-J-induced tumor livers were collected to detect core gene expression levels to provide a proof to the concluded tumorigenic mechanism. Together, our results suggest that circ-Vav3/gga-miR-375/YAP1 axis is another regulator of tumorigenesis.

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“…Generally, circRNAs regulate gene expression by sponging miRNAs . By the means of dual luciferase reporter assay and RIP, miR‐296‐5p was validated as the direct binding target for hsa_circ_001895.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

et al. 2019

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This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. AbstractThere is an urgent need to find novel potential therapeutic targets for the diagnosis and treatment of clear cell renal cell carcinoma (ccRCC) due to its highly invasive ability as a common urological malignant tumor. Circular RNAs (circRNAs) have been indicated as potentially critical mediators in various types of tumor progression. We

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microRNA sponging by a new hearty circRNA

Cited by 4 publications

References 0 publications

CircPCNXL2 sponges miR-153 to promote the proliferation and invasion of renal cancer cells through upregulating ZEB2

CircPCNXL2 sponges miR-153 to promote the proliferation and invasion of renal cancer cells through upregulating ZEB2

Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition

Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

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