MicroRNA Signature in Human Normal and Tumoral Neural Stem Cells

Diana, Andrea; Gaido, Giuseppe; Murtas, Daniela

doi:10.3390/ijms20174123

Cited by 24 publications

(31 citation statements)

References 123 publications

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“…Compared to normal NSCs, cancerous NSCs upregulate oncogenic miRNAs, including miR-9, 17, 21, 106b, and 221, and downregulate tumor suppressor miRNAs, including miR-34a, 124, 137, 146a, and 152. 113 The relationship of Ab and miRNAs in AD was also reviewed. 114 The major miRNAs that are upregulated in AD include miR-21, 34a, 122, 126, 130b, 183, and 206 (i.e., most are neurodegenerative miRNAs).…”

Section: The Physiopathology Of Evs For Understanding Disease Mechanimentioning

confidence: 99%

Human Pluripotent Stem Cell-Derived Extracellular Vesicles: Characteristics and Applications

Jeske

Bejoy

Marzano

et al. 2020

Tissue Engineering Part B: Reviews

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Extracellular vesicles (EVs), including exosomes and microvesicles, are found to play an important role in various biological processes and maintaining tissue homeostasis. Because of the protective effects, stem cellderived EVs can be used to reduce oxidative stress and apoptosis in the recipient cells. In addition, EVs/ exosomes have been used as directional communication tools between stem cells and parenchymal cells, giving them the ability to serve as biomarkers. Likewise, altered EVs/exosomes can be utilized for drug delivery by loading with proteins, small interfering RNAs, and viral vectors, in particular, because EVs/exosomes are able to cross the blood-brain barrier. In this review article, the properties of human induced pluripotent stem cell (iPSC)-derived EVs are discussed. The biogenesis, that is, how EVs originate in the endosomal compartment or from the cell layer of microvesicles, EV composition, the available methods of purification, and characterizations of EVs/exosomes are summarized. In particular, EVs/exosomes derived from iPSCs of different lineage specifications and the applications of these stem cell-derived exosomes in neurological diseases are discussed.

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Section: The Physiopathology Of Evs For Understanding Disease Mechanimentioning

confidence: 99%

Human Pluripotent Stem Cell-Derived Extracellular Vesicles: Characteristics and Applications

Jeske

Bejoy

Marzano

et al. 2020

Tissue Engineering Part B: Reviews

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show abstract

“…Since a single miRNA can act on hundreds or thousands of target genes, the alternation of its expression not only lead to changes of the corresponding target gene expression level, but also can cause cascade radiation-like changes in the cell through protein–protein interactions, just like the butterfly effect, and ultimately even fundamentally convert the cell fate. In the past few years, many miRNAs have been demonstrated to be involved in the stemness maintenance of NSCs 19 and the differentiation of MNs 7 . But the specific details and cascade of their roles are still unclear.…”

Section: Discussionmentioning

confidence: 99%

mRNA and miRNA expression profile reveals the role of miR-31 overexpression in neural stem cell

Gao

Xiao

et al. 2020

Sci Rep

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A detailed understanding of the character and differentiation mechanism of neural stem cells (NSCs) will help us to effectively utilize their transplantation to treat spinal cord injury. In previous studies, we found that compared with motor neurons (MNs), miR-31 was significantly high-expressed in NSCs and might play an important role in the proliferation of NSCs and the differentiation into MNs. To better understand the role of miR-31, we characterized the mRNA and miRNAs expression profiles in the early stage of spinal cord-derived NSCs after miR-31 overexpression. There were 35 mRNAs and 190 miRNAs differentially expressed between the miR-31 overexpression group and the control group. Compared with the control group, both the up-regulated mRNAs and miRNAs were associated with the stemness maintenance of NSCs and inhibited their differentiation, especially to MNs, whereas the down-regulated had the opposite effect. Further analysis of the inhibition of miR-31 in NSCs showed that interfering with miR-31 could increase the expression of MNs-related genes and produce MNs-like cells. All these indicated that miR-31 is a stemness maintenance gene of NSCs and has a negative regulatory role in the differentiation of NSCs into MNs. This study deepens our understanding of the role of miR-31 in NSCs, provides an effective candidate target for effectively inducing the differentiation of NSCs into MNs, and lays a foundation for the effective application of NSCs in clinic.

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“…7,28 It is detected as one of the miRNAs forming "miR signature" in neural stem and neural cancer stem cell. 32 In our experiments, A1235 cells with high expression of mature miR-7 were obtained. The cells were transfected with a miR DNA sequence, and the miR processing was dependent on the cellular machinery.…”

Section: Discussionmentioning

confidence: 99%

miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line

Kolić

Horvat

Šetinc

et al. 2020

Mol. exp. biol. med.

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miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents.

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MicroRNA Signature in Human Normal and Tumoral Neural Stem Cells

Cited by 24 publications

References 123 publications

Human Pluripotent Stem Cell-Derived Extracellular Vesicles: Characteristics and Applications

Human Pluripotent Stem Cell-Derived Extracellular Vesicles: Characteristics and Applications

mRNA and miRNA expression profile reveals the role of miR-31 overexpression in neural stem cell

miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line

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