microRNA s (9, 138, 181A, 221, and 222) and mesial temporal lobe epilepsy in developing brains

Ashhab, Muhammad Usman; Omran, Ahmed; Kong, Huimin; Peng, Jing; Yin, Fei

doi:10.2478/s13380-013-0128-z

Cited by 8 publications

(11 citation statements)

References 35 publications

(32 reference statements)

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“…In the intervening years, six studies have undertaken the miRNA expression profiling either in brain tissue samples (Kan et al, 2012 ; McKiernan et al, 2012b ; Kaalund et al, 2014 ; Zucchini et al, 2014 ) or serum (Wang et al, 2015a , b ) of patients with epilepsy (Table 2 ), along with study on individual miRNAs in human epilepsy (Aronica et al, 2010 ; Jimenez-Mateos et al, 2012 ; Omran et al, 2012 ; Ashhab et al, 2013a , b ; Peng et al, 2013 ; Alsharafi and Xiao, 2015 ). Specifically, miRNAs expression profiles were performed on patients with mesial temporal lobe epilepsy (mTLE) (Kan et al, 2012 ; Kaalund et al, 2014 ), temporal lobe epilepsy (TLE) (McKiernan et al, 2012b ), or Drug-resistant epilepsy (Zucchini et al, 2014 ; Wang et al, 2015b ).…”

Section: Mirna Alterations In Epilepsymentioning

confidence: 99%

“…In 2013, Ashhab and colleagues aimed to explore the dynamic expression of miR-221 and miR-222 in the three phases of TLE and in children with TLE. They demonstrated that both miR-221 and miR-222 were downregulated in the three phases of TLE in animal models and in children with epilepsy (Ashhab et al, 2013a ). In the same study, inflammation-related miR-138 was decreased in the seizure-related phase but not in the latent phase, whereas inflammation-related miR-181a was decreased in the acute phase, normal in the latent phase and increased in the chronic phase (Ashhab et al, 2013a ).…”

Section: Therapeutic Aspects Of Mirnasmentioning

confidence: 99%

“…They demonstrated that both miR-221 and miR-222 were downregulated in the three phases of TLE in animal models and in children with epilepsy (Ashhab et al, 2013a ). In the same study, inflammation-related miR-138 was decreased in the seizure-related phase but not in the latent phase, whereas inflammation-related miR-181a was decreased in the acute phase, normal in the latent phase and increased in the chronic phase (Ashhab et al, 2013a ). In our previous work, we investigated the dynamic expression pattern of inflammation-related miR-128, miR-30c, and miR-27a in the three phases of TLE as well as in patients with TLE.…”

Section: Therapeutic Aspects Of Mirnasmentioning

confidence: 99%

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miRNAs: biological and clinical determinants in epilepsy

Alsharafi

Xiao

Abuhamed

et al. 2015

Front. Mol. Neurosci.

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Recently, microRNAs (miRNAs) are reported to be crucial modulators in the pathogenesis and potential treatment of epilepsies. To date, several miRNAs have been demonstrated to be significantly expressed in the epileptic tissues and strongly associated with the development of epilepsy. Specifically, miRNAs regulate synaptic strength, inflammation, neuronal and glial function, ion channels, and apoptosis. Furthermore, peripheral blood miRNAs can also be utilized as diagnostic biomarkers to assess disease risk and treatment responses. Here, we will summarize the recent available literature regarding the role of miRNAs in the pathogenesis and treatment of epilepsy. Moreover, we will provide brief insight into the potential of miRNA as diagnostic biomarkers for early diagnosis and prognosis of epilepsy.

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Section: Mirna Alterations In Epilepsymentioning

confidence: 99%

Section: Therapeutic Aspects Of Mirnasmentioning

confidence: 99%

Section: Therapeutic Aspects Of Mirnasmentioning

confidence: 99%

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miRNAs: biological and clinical determinants in epilepsy

Alsharafi

Xiao

Abuhamed

et al. 2015

Front. Mol. Neurosci.

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“…miR-138 is a neuronspecific miRNA, which dynamically regulates neural development by controlling the shape and size of dendrites and thereby influences long-term memory [24]. miR-138 was also found to be downregulated in the seizure-related stages of the mesial temporal lobe epilepsy (MTLE) model in the developing brains [10]. [14].…”

Section: Discussionmentioning

confidence: 97%

“…miRNAs are also involved in the regulation of several aspects of the innate, adaptive immune responses and inflammationrelated disorders. Studies uncovered a subset of miRNAs which are brain-enriched including miRNAs (124, 134, 9, and 138) and inflammationrelated miRNAs including (132, 181a, 221, and 222) which were extensively studied in multiple brain disease in both developing and adult brains [4,[10][11][12], and also in infectious diseases [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs expression changes in acute Streptococcus pneumoniae meningitis

Omran¹,

Jagoo

Ashhab

et al. 2014

Translational Neuroscience

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Background: Despite prompt and appropriate care, Streptococcus pneumoniae (S. pneumoniae) meningitis remains a key cause of childhood morbidity and mortality. Recently, several studies suggested the possibility of microRNAs (miRNA) involvement in multiple brain and infectious diseases. This study aimed to investigate the expressional changes of brain-enriched miRNAs (124, 134, 9, and 138), and inflammation-related miRNAs (132, 181a, 221, and 222) in the cerebral cortex of acute S. pneumoniae meningitis in postnatal day 25 rats with and without treatment. Methodology: Quantitative polymerase chain reaction (qPCR) was used to measure the expression levels of the tested brain-enriched and inflammation-related miRNAs in the cerebral cortical tissues obtained from acute experimental meningitis rat model induced by intracranial inoculation of S. pneumoniae serotype 3 with and without treatment. Control rats inoculated with saline were also included. Results: Brainenriched miRNAs are significantly downregulated in untreated animals while after treatment with antibiotic and steroid for 24 hours, miR-124 and miR-9 expressions were nearly equal to the control, while miR-134 and miR-138 were significantly upregulated. Inflammation-related miR-132 was significantly downregulated in untreated and treated animals while miRNAs (181a, 221, and 222) were significantly upregulated in untreated and treated animals. Conclusion: Acute S. pneumoniae meningitis leads to significant changes in the cerebral cortical expressions of some brain-enriched and inflammation-related miRNAs. These miRNAs may play a role in the pathogenesis of acute bacterial meningitis.

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Changes in Microglial Inflammation-Related and Brain-Enriched MicroRNAs Expressions in Response to In Vitro Oxygen–Glucose Deprivation

et al. 2013

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Microglia plays important role in central nervous system immune surveillance and has emerged as an essential cellular component for understanding brain diseases. MicroRNAs (miRs) are small, noncoding RNAs that regulate the post-transcriptional expression of protein-coding mRNAs, which may have key roles in microglial activation in response to brain ischemia and other stressors. Primary cultured rat microglial cells were prepared, and then microglial activation model was established by oxygen-glucose deprivation (OGD) method. Morphological observation, CD11b/c immunofluorence, MTT assay and Propidium iodide staining were done to test microglia viability at different OGD time points (0, 5, 10, 15, 30, 60 min). qPCR were performed to detect the dynamic changes in expressions of inflammation-related miRs (146a, 21, 181a, 221, and 222) and brain-enriched miRs (124, 134, 9, 132, and 138) in resting microglia and after challenge with OGD for the same time points. The activation and viability of the microglia was time dependent. Similarly, expressions of different miRs in microglia were significantly upregulated and reached the peak at different time points before reaching the baseline level with extension of OGD. Our data demonstrates for the first time that OGD as a model of an ischemic insult modulates the expressions of some inflammation-related and brain-enriched miRs. These changes may help to explore the molecular basis of microglia activation on the post-transcriptional level in response to different time points of OGD.

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microRNA s (9, 138, 181A, 221, and 222) and mesial temporal lobe epilepsy in developing brains

Cited by 8 publications

References 35 publications

miRNAs: biological and clinical determinants in epilepsy

miRNAs: biological and clinical determinants in epilepsy

MicroRNAs expression changes in acute Streptococcus pneumoniae meningitis

Changes in Microglial Inflammation-Related and Brain-Enriched MicroRNAs Expressions in Response to In Vitro Oxygen–Glucose Deprivation

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