microRNA regulation of fruit growth

Ripoll, Juan-José; Bailey, Lindsay J.; Mai, Quynh-Anh; Wu, Scott; Hon, Cindy T; Chapman, Elisabeth J.; Ditta, Gary S.; Estelle, Mark; Yanofsky, Martin F.

doi:10.1038/nplants.2015.36

Cited by 121 publications

(137 citation statements)

References 74 publications

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“…In Arabidopsis , miR172 regulates the elongation of the valve under the modulation of the FUL gene (a MADS-box gene) and the ARF6/8 gene (José Ripoll et al, 2015). Here we showed that miR172 regulated floral organ development in rice, especially the elongation of the palea and lemma, under the modulation of OsMADS1 .…”

Section: Discussionmentioning

confidence: 99%

OsMADS1 Represses microRNA172 in Elongation of Palea/Lemma Development in Rice

et al. 2016

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Specification of floral organ identity is critical for the establishment of floral morphology and inflorescence architecture. Although multiple genes are involved in the regulation of floral organogenesis, our understanding of the underlying regulating network is still fragmentary. MADs-box genes are principle members in the ABCDE model that characterized floral organs. OsMADS1 specifies the determinacy of spikelet meristem and lemma/palea identity in rice. However, the pathway through which OsMADS1 regulates floral organs remains elusive; here, we identified the microRNA172 (miR172) family as possible regulators downstream of OsMADS1. Genetic study revealed that overexpression of each miR172 gene resulted in elongated lemma/palea and indeterminacy of the floret, which resemble the phenotype of osmads1 mutant. On the contrary, overexpression of each target APETALA2 (AP2) genes resulted in shortened palea/lemma. Expression level and specificity of miR172 was greatly influenced by OsMADS1, as revealed by Northern blot analysis and In situ hybridization. Genetically, AP2-3 and AP2-2 over expression rescued the elongation and inconsistent development of the lemma/palea in OsMADS1RNAi transgenic plants. Our results suggested that in rice, OsMADS1 and miR172s/AP2s formed a regulatory network involved in floral organ development, particularly the elongation of the lemma and the palea.

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Section: Discussionmentioning

confidence: 99%

OsMADS1 Represses microRNA172 in Elongation of Palea/Lemma Development in Rice

et al. 2016

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“…Similarly, the overexpression of apple Md‐miRNA156 in Arabidopsis resulted in late flowering, dwarfism, more leaves, short siliques with partially aborted seeds, by down‐regulating its target SPL genes (Sun et al , ). As the downstream of FUL and ARF6/8 , the miR172 plays a key role in regulating fruit development, as it is required for valve growth by restricting AP2 and TOE3 activity (José Ripoll et al , ). The reduced lignin deposition and increased silique number/length and seed size/yield was observed in transgenic Arabidopsis overexpressing miR397b , which regulated a laccase gene LAC4 that can polymerize monolignols into lignin (Wang et al , ).…”

Section: Micrornamentioning

confidence: 99%

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Hussain

Shi

Scheben

et al. 2020

Plant Biotechnology Journal

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Summary Fruit is seed‐bearing structures specific to angiosperm that form from the gynoecium after flowering. Fruit size is an important fitness character for plant evolution and an agronomical trait for crop domestication/improvement. Despite the functional and economic importance of fruit size, the underlying genes and mechanisms are poorly understood, especially for dry fruit types. Improving our understanding of the genomic basis for fruit size opens the potential to apply gene‐editing technology such as CRISPR/Cas to modulate fruit size in a range of species. This review examines the genes involved in the regulation of fruit size and identifies their genetic/signalling pathways, including the phytohormones, transcription and elongation factors, ubiquitin‐proteasome and microRNA pathways, G‐protein and receptor kinases signalling, arabinogalactan and RNA‐binding proteins. Interestingly, different plant taxa have conserved functions for various fruit size regulators, suggesting that common genome edits across species may have similar outcomes. Many fruit size regulators identified to date are pleiotropic and affect other organs such as seeds, flowers and leaves, indicating a coordinated regulation. The relationships between fruit size and fruit number/seed number per fruit/seed size, as well as future research questions, are also discussed.

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“…MicroRNA172 ( miRNA172 ) inhibits the translation of a subfamily of APETALA2 ( AP2 ) genes (Chen, ) that govern floral organ development (Yant et al ., ) and organ size (Jofuku et al ., ) in Arabidopsis, and a recent study has shown that miRNA172 positively regulates Arabidopsis silique size (Ripoll et al ., ). As apple fruit develop from more than one type of floral organs (Pratt, ), miRNA172 was an attractive candidate to study in relation to its potential role in the regulation of apple fruit size.…”

Section: Introductionmentioning

confidence: 97%

AmicroRNAallele that emerged prior to apple domestication may underlie fruit size evolution

Yao

Cornille

et al. 2015

The Plant Journal

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SUMMARYThe molecular genetic mechanisms underlying fruit size remain poorly understood in perennial crops, despite size being an important agronomic trait. Here we show that the expression level of a microRNA gene (miRNA172) influences fruit size in apple. A transposon insertional allele of miRNA172 showing reduced expression associates with large fruit in an apple breeding population, whereas over-expression of miRNA172 in transgenic apple significantly reduces fruit size. The transposon insertional allele was found to be co-located with a major fruit size quantitative trait locus, fixed in cultivated apples and their wild progenitor species with relatively large fruit. This finding supports the view that the selection for large size in apple fruit was initiated prior to apple domestication, likely by large mammals, before being subsequently strengthened by humans, and also helps to explain why signatures of genetic bottlenecks and selective sweeps are normally weaker in perennial crops than in annual crops.

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microRNA regulation of fruit growth

Cited by 121 publications

References 74 publications

OsMADS1 Represses microRNA172 in Elongation of Palea/Lemma Development in Rice

OsMADS1 Represses microRNA172 in Elongation of Palea/Lemma Development in Rice

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

AmicroRNAallele that emerged prior to apple domestication may underlie fruit size evolution

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