MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo

Henn, Dominic; Abu‐Halima, Masood; Wermke, Dominik; Falkner, Frank; Thomas, Benjamin; Köpple, Christoph; Ludwig, Nicole; Schulte, Matthias; Brockmann, Marc A.; Kim, Yoo Jin; Sacks, Justin M.; Kneser, Ulrich; Keller, Andreas; Meese, Eckart; Schmidt, Volker

doi:10.1186/s12967-019-1767-9

Cited by 37 publications

(29 citation statements)

References 82 publications

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“…The relative abundance level for each miRNA was calculated using the 2 −ΔΔCT equation [23]. RNU6B small nuclear RNA (snRNA) was used an endogenous reference control for normalization purpose as previously described [12,13,22,[24][25][26][27] and because of its minimum abundance variance between the TS patients and HVs as observed by DataAssist™Software (Applied Biosystems). Correlations were computed using Spearman's regression coefficient and the difference between the groups was analyzed using a Mann-Whitney-U test using GraphPad Prism 7.0.…”

Section: Resultsmentioning

confidence: 99%

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

et al. 2020

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Background Turner syndrome (TS) is a chromosomal disorder, in which a female is partially or entirely missing one of the two X chromosomes, with a prevalence of 1:2500 live female births. The present study aims to identify a circulating microRNA (miRNA) signature for TS patients with and without congenital heart disease (CHD). Methods Microarray platform interrogating 2549 miRNAs were used to detect the miRNA abundance levels in the blood of 33 TS patients and 14 age-matched healthy volunteer controls (HVs). The differentially abundant miRNAs between the two groups were further validated by RT-qPCR. Results We identified 60 differentially abundant miRNA in the blood of TS patients compared to HVs, from which, 41 and 19 miRNAs showed a higher and a lower abundance levels in TS patients compared to HVs, respectively. RT-qPCR confirmed the significantly higher abundance levels of eight miRNAs namely miR-374b-5p, miR-199a-5p, miR-340-3p, miR-125b-5p, miR-30e-3p, miR-126-3p, miR-5695, and miR-26b-5p in TS patients as compared with the HVs. The abundance level of miR-5695 was higher in TS patients displaying CHD as compared to TS patients without CHD (p = 0.0265; log2-fold change 1.99); whereas, the abundance level of miR-126-3p was lower in TS patients with congenital aortic valve disease (AVD) compared to TS patients without BAV (p = 0.0139, log2-fold change 1.52). The clinical feature statistics revealed that miR-126-3p had a significant correlation with sinotubular junction Z-score (r = 0.42; p = 0.0154).

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Section: Resultsmentioning

confidence: 99%

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

et al. 2020

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“…Previous studies have demonstrated that miR-124 plays a key role in multiple diseases including IPF by targeting SMAD5, CAV1, JAG1, ROCK1 or STAT3 [37][38][39][40][41][42], which is consistent with our findings. Previous studies have also shown that FOXC1 and HSPA1A are direct target genes of miR-223 [43,44], and these genes contribute to the pathogenesis of IPF via activating various signaling pathways. Our present results suggested that down-regulated hsa_circ_0029633 might promote the development of IPF by increasing the expression of miR-223, and further decreasing the expression of FOXC1 and HSPA1A.…”

Section: Discussionmentioning

confidence: 95%

Potential function and molecular mechanism of circRNAs involved in idiopathic pulmonary fibrosis

Wang²,

Tao³

et al. 2020

Preprint

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Background: Recent studies have found a regulatory role of circular RNAs (circRNAs) in the pathogenesis of idiopathic pulmonary fibrosis (IPF). However, the function and underlying molecular mechanism of circRNAs involved in IPF are uncertain and incomplete. This study aimed to further provide some critical information for the circRNA function in IPF using bioinformatic analysis.Methods: We searched in the NCBI (National Center for Biotechnology Information) Gene Expression Omnibus (GEO) database to find the circRNA expression profiles of human IPF. The microarray data GSE102660 was obtained and differentially expressed circRNAs were identified through R software.Results: 6 significantly up-regulated and 13 significantly down-regulated circRNAs were identified involved in the pathogenesis of IPF. The binding sites of miRNAs for each differentially expressed circRNA were also predicted and circRNA-miRNA-mRNA networks were constructed for the most upregulated hsa_circ_0004099 and down-regulated hsa_circ_0029633. In addition, GO and KEGG enrichment analysis revealed the molecular function and enriched pathways of the target genes of circRNAs in IPF.Conclusion: These findings suggest that candidate circRNAs might serve an important role in the pathogenesis of IPF. Therefore, these circRNAs might be potential biomarkers for diagnosis and promising targets for treatment of IPF, which still need further verification in vivo and in vitro.

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“…CXCL2 was involved in various biological progresses, such as angiogenesis, inflammation and cancer biological behaviors (23,(27)(28)(29)(30). CXCL2 was also considered to be a proinflammatory factor in RA (24).…”

Section: Discussionmentioning

confidence: 99%

RNA sequencing analysis reveals differential gene expression of CXCL2 in ACPA-positive and ACPA-negative rheumatoid arthritis

Sun¹,

Wang²,

He³

et al. 2020

Preprint

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Background. Anti-citrullinated protein/peptide antibodies (ACPA) play important roles in the pathogenesis of rheumatoid arthritis (RA), and are associated with RA severity. It has been suggested that ACPA-positive (ACPA+) and ACPA-negative (ACPA-) RA are different disease subsets with distinct differences in genetic variation and clinical outcomes. The aims of the present study were to compare gene expression profiles in ACPA + and ACPA- RA and identify novel candidate gene signatures that might serve as therapeutic targets. Methods. Comprehensive transcriptome analysis of peripheral blood mononuclear cells (PBMCs) from ACPA + and ACPA- RA patients, and healthy controls was performed via RNA sequencing. Genes with significantly different expressions were analyzed by cluster analysis, Gene Ontology analysis and Ingenuity Pathway analysis. A validation cohort was used to further investigate differentially expressed genes via real-time PCR and enzyme-linked immunosorbent assay. Spearman's correlation test was used to evaluate the correlation of differentially expressed genes and the clinical and laboratory data of the patients. The role of differentially expressed genes in osteoclastogenesis was further investigated. Results. There were significant differences in the expression levels of both genes and gene isoforms between ACPA + and ACPA- RA samples. Expression of C-X-C motif chemokine ligand 2 (CXCL2) was significantly increased in ACPA + RA patients than in ACPA- RA patients and healthy controls. Validation of candidate genes expression showed that CXCL2 levels in PBMCs and serum were higher in ACPA + RA patients than in ACPA- RA patients and healthy controls. CXCL2 promoted the migration of CD14 + monocytes and increased osteoclast differentiation in RA patients. RAW264.7 macrophages were used to investigate specific mechanisms, and the results suggested that CXCL2 stimulated osteoclastogenesis via ERK MAPK and NFκB pathways. Conclusion. Novel pathways associated with ACPA + RA were identified via RNA sequencing, and CXCL2 was highly expressed in ACPA + RA than in ACPA- RA. These results reveal a previously unreported role of CXCL2 during osteoclastogenesis in RA, and suggest that the blockade of CXCL2 might be a novel strategy for the treatment of RA.

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MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo

Cited by 37 publications

References 82 publications

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

Potential function and molecular mechanism of circRNAs involved in idiopathic pulmonary fibrosis

RNA sequencing analysis reveals differential gene expression of CXCL2 in ACPA-positive and ACPA-negative rheumatoid arthritis

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