MicroRNA Profiling in Mid- and Late-Gestational Fetal Skin: Implication for Scarless Wound Healing

Cheng, Jie; Yu, Hongbo; Deng, Simin; Shen, Guofang

doi:10.1620/tjem.221.203

Cited by 50 publications

(44 citation statements)

References 39 publications

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“…The healing at this stage featured adult phenotype, including scar formation. 7 Of note in this context is the observation that miRNA biogenesis is substantially blunted at E16, indicative of dampened PTGS in the fetal skin. 7 Global suppression of miRNA function has been consistently noted in mouse oocytes and early embryo.…”

Section: Development Is the Key To Regenerationmentioning

confidence: 99%

“…7 Of note in this context is the observation that miRNA biogenesis is substantially blunted at E16, indicative of dampened PTGS in the fetal skin. 7 Global suppression of miRNA function has been consistently noted in mouse oocytes and early embryo. 8 The global suppression of miRNA during mouse oocyte-to-embryo transition is likely facilitated by the expression of highly conserved RNA binding proteins Lin28a and Lin28b that are abundant during embryogenesis.…”

Section: Development Is the Key To Regenerationmentioning

confidence: 99%

“…Comparison of the global expression pattern of mature miRNA of mouse fetal skin from different developmental stages has provided critical insight into miRNA-based regulation of adult tissue development. 7 Healing of the skin at embryonic day (E)16 is regenerative and scarless and was associated with global miRNA repression compared with the skin of E19. The healing at this stage featured adult phenotype, including scar formation.…”

Section: Development Is the Key To Regenerationmentioning

confidence: 99%

“…In mouse fetal skin, Dicer is substantially down-regulated, indicating suppressed global miRNA biogenesis that is likely to prevent silencing of numerous coding genes aimed at tissue regeneration often evident at E16. 7 Dicer acts as a molecular ruler that cleaves the pre-miRNA at a specific distance from the ends produced by Drosha cleavage and generating an approximate 22-nt double-stranded miRNA duplex (miR/miR*) with a 2-nt 3 0 overhang. Dicer forms the RNA induced silencing complex (RISC) in combination with TAR RNA binding protein (TRBP), protein kinase RNA activator (PACT), and Argonaute (AGO) proteins.…”

Section: Mirna Biogenesismentioning

confidence: 99%

“…Of such miRNAs that are silenced during fetal development, low abundance of miR-29 was reported in skin across several species. 7,75,76 In humans, hsa-miR-29a and hsa-miR-29b-1 (chromosome 7) and hsa-miR-29b-2 and hsa-miR-29c (chromosome 1) are highly conserved members of the miR-29 family that share the sequence homology at the seed region (nts 2-7) that decides the fate of coding genes. PTGS of multiple ECM proteins by the miR-29a-c family is one notable example of a single miRNA family regulating multiple ECM proteins such as different collagen isoforms, laminin 1, fibrilin 1, elastin, matrix metalloproteinase 2, hyaluronic acid, chondroitin sulfate, and integrin 1.…”

Section: Mirna and Regenerative Medicinementioning

confidence: 99%

See 4 more Smart Citations

miRNA Control of Tissue Repair and Regeneration

Sen

Ghatak

2015

The American Journal of Pathology

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Section: Development Is the Key To Regenerationmentioning

confidence: 99%

Section: Development Is the Key To Regenerationmentioning

confidence: 99%

Section: Development Is the Key To Regenerationmentioning

confidence: 99%

Section: Mirna Biogenesismentioning

confidence: 99%

Section: Mirna and Regenerative Medicinementioning

confidence: 99%

See 3 more Smart Citations

miRNA Control of Tissue Repair and Regeneration

Sen

Ghatak

2015

The American Journal of Pathology

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Scarless integumentary wound healing in the mammalian fetus: Molecular basis and therapeutic implications

Kathju

Gallo

Satish

2012

Birth Defects Research Pt C

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Adult mammals respond to injury of their skin/integument by forming scar tissue. Scar is useful in rapidly sealing an injured area, but can also lead to significant morbidity. Mammals in fetal life retain the ability to heal integumentary wounds regeneratively, without scar. The critical molecular mechanisms governing this remarkable phenomenon have been a subject of great interest, in the hopes that these could be dissected and recapitulated in the healing adult wound, with the goal of inducing scarless healing in injured patients. Multiple lines of investigation spanning decades have implicated a number of factors in distinguishing scarless from fibrotic wound healing, including most prominently transforming growth factor-β and interleukin-10, among others. Therapeutic interventions to try to mitigate scarring in adult wounds have been developed out of these studies, and have reached the level of clinical trials in humans, although as yet no FDA-approved treatment exists. More recent expressomic studies have revealed many more genes that are differentially expressed in scarlessly healing fetal wounds compared with adult, and microRNAs have also been identified as participating in the fetal wound healing response. These represent an even greater range of potential therapeutics (or targets for therapy) to translate the promise of scarless fetal wound healing to the injured adult patient.

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Drosophila as a model of wound healing and tissue regeneration in vertebrates

Belacortu

Paricio

2011

Developmental Dynamics

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Understanding the molecular basis of wound healing and regeneration in vertebrates is one of the main challenges in biology and medicine. This understanding will lead to medical advances allowing accelerated tissue repair after wounding, rebuilding new tissues/organs and restoring homeostasis. Drosophila has emerged as a valuable model for studying these processes because the genetic networks and cytoskeletal machinery involved in epithelial movements occurring during embryonic dorsal closure, larval imaginal disc fusion/regeneration, and epithelial repair are similar to those acting during wound healing and regeneration in vertebrates. Recent studies have also focused on the use of Drosophila adult stem cells to maintain tissue homeostasis. Here, we review how Drosophila has contributed to our understanding of these processes, primarily through live-imaging and genetic tools that are impractical in mammals. Furthermore, we highlight future research areas where this insect may provide novel insights and potential therapeutic strategies for wound healing and regeneration. Developmental Dynamics 240:2379-2404,

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MicroRNA Profiling in Mid- and Late-Gestational Fetal Skin: Implication for Scarless Wound Healing

Cited by 50 publications

References 39 publications

miRNA Control of Tissue Repair and Regeneration

miRNA Control of Tissue Repair and Regeneration

Scarless integumentary wound healing in the mammalian fetus: Molecular basis and therapeutic implications

Drosophila as a model of wound healing and tissue regeneration in vertebrates

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