microRNA Profiling in Glaucoma Eyes With Varying Degrees of Optic Neuropathy by Using Next-Generation Sequencing

Liu, Yaoming; Chen, Yang; Wang, Yayi; Zhang, Xinyu; Gao, Xinbo; Chen, Shida; Zhang, Xiulan

doi:10.1167/iovs.17-23599

Cited by 47 publications

(59 citation statements)

References 63 publications

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“…25 In our previous study, we also used NGS to explore the miRNA profile in the AH of glaucoma patients, and we found glaucoma specific miRNAs profile and related to glaucoma progression. 19 The finding of PDR-specific miRNAs in our study was consistent with other studies [26][27][28] (as shown in Table 3). Recently, miR-150-5p was found to be strongly downregulated in the extracellular vesicles of blood from DR subjects using miRNA array detection 13 and suppression of the pathological neovascularization in mice using oxygeninduced proliferative retinopathy.…”

Section: Discussionsupporting

confidence: 92%

“…Thus, miRNAs were proposed as biomarkers, and the expression profile of circulating miRNAs has been evaluated in cancer, cardiovascular disease, diabetes and ocular disease . In our previous study, we also used NGS to explore the miRNA profile in the AH of glaucoma patients, and we found glaucoma specific miRNAs profile and related to glaucoma progression …”

Section: Discussionmentioning

confidence: 95%

“…Synthetic Caenorhabditis elegans miR‐39 spike‐in control was added to the supernatant of the aqueous during RNA extraction. The NGS of small RNA (sRNA) in the AH was conducted as in our previous study . RNA from each aqueous sample was used to generate sequencing libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs Inc, Ipswich, Massachusetts).…”

Section: Methodsmentioning

confidence: 99%

“…The NGS of small RNA (sRNA) in the AH was conducted as in our previous study. 19 RNA from each aqueous sample was used to generate sequencing libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs Inc, Ipswich, Massachusetts). After selecting the size of 18-30 nucleotide RNA using polyacrylamide gel electrophoresis (PAGE) gel, adapter-ligated libraries were reverse transcribed and amplified by PCR.…”

Section: Microrna Sequencing and Bioinformatics Analysismentioning

confidence: 99%

“…miRNAs constitute a large family of small (19)(20)(21)(22) nucleotides long), non-coding RNAs that act as post-transcriptional regulators of gene expression. 6 Due to their stability in biofluids such as blood, urine, and aqueous humour (AH), 7 as well as their cell-type and tissue specificity, miRNAs are potential biomarkers for the detection of DR. 4 A few studies have detected miRNAs in the blood or vitreous humour of DR patients using microarray or polymerase chain reaction (PCR), and suggested that miRNA may be involved in DR and that some miRNAs may be biomarkers for DR. [8][9][10][11][12][13] However, miRNA arrays or PCR have only been able to detect some of the mature miRNAs in the known miRbase database.…”

Section: Introductionmentioning

confidence: 99%

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Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Chen

Yuan

Liu

et al. 2019

Clinical Exper Ophthalmology

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Background The microRNAs (miRNA) have been found to play an important role in the pathogenesis of diabetic retinopathy. We try to explore the miRNA and piwi‐interacting RNA (piRNA) profile in the aqueous humour of proliferative diabetic retinopathy (PDR) using next‐generation sequencing (NGS). Methods Aqueous humour samples were collected from nine PDR eyes and nine cataract control eyes, and NGS was performed. Quantitative polymerase chain reaction (qPCR) was used to validate the sequencing results. An oxygen‐induced retinopathy (OIR) model was used to validate the angiogenesis related miRNA. Results In total, 484 miRNAs were differently expressed between the PDR eyes and cataract control eyes, including 210 mature miRNAs and 274 novel miRNAs. Furthermore, eight miRNAs and 30 piRNAs were identified as the most differently expressed between the two groups (P > .85). This differential expression of miRNA was predicted to regulate Rho protein signal transduction, neurotransmitter uptake and histone lysine methylation. Relative expression patterns of miR‐184, ‐150‐5p and ‐93‐5p were confirmed by qPCR. A reduced expression of miR‐93‐5p was confirmed in the OIR model. Conclusions This study comprehensively demonstrated the miRNA and piRNA expression profile of the aqueous humour of PDR eyes, which may serve as a potential biomarker and involved in the pathogenesis of PDR.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Microrna Sequencing and Bioinformatics Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Chen

Yuan

Liu

et al. 2019

Clinical Exper Ophthalmology

Self Cite

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Insights into the regulatory molecules involved in glaucoma pathogenesis

Moazzeni

Khani

Elahi

2020

American J of Med Genetics Pt C

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Glaucoma is an important cause of irreversible blindness, characterized by optic nerve anomalies. Increased intraocular pressure (IOP) and aging are major risk factors. Retinal ganglion cells and trabecular meshwork cells are certainly involved in the etiology of glaucoma. Glaucoma is usually a complex disease, and various genes and functions may contribute to its etiology. Among these may be genes that encode regulatory molecules. In this review, regulatory molecules including 18 transcription factors (TFs), 195 microRNAs (miRNAs), 106 long noncoding RNAs (lncRNAs), and two circular RNAs (circRNAs) that are reasonable candidates for having roles in glaucoma pathogenesis are described. The targets of the regulators are reported. Glaucomarelated features including apoptosis, stress responses, immune functions, ECM properties, IOP, and eye development are affected by the targeted genes. The targeted genes that are frequently targeted by multiple regulators most often affect apoptosis and the related features of cell death and cell survival. BCL2, CDKN1A, and TP53 are among the frequent targets of three types of glaucoma-relevant regulators, TFs, miRNAs, and lncRNAs. TP53 was itself identified as a glaucoma-relevant TF. Several of the glaucoma-relevant TFs are themselves among frequent targets of regulatory molecules, which is consistent with existence of a complex network involved in glaucoma pathogenesis.

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Pathogenesis and prospects for therapeutic clinical application of noncoding RNAs in glaucoma: Systematic perspectives

Rong

Wang

You

et al. 2021

Journal Cellular Physiology

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Noncoding ribonucleic acids (ncRNAs) are an increasingly studied class of RNA molecules with extensive biological activities, including important roles in human development, health, and disease. Glaucoma is a neurodegenerative disease of the retina, and one of the leading causes of blindness worldwide. However, the specific roles of ncRNAs in the development and progression of glaucoma are unclear, and related reports are fragmented. An in‐depth understanding of ncRNAs participating in the pathogenesis and progression of glaucoma would be helpful for opening up new avenues to facilitate the early diagnosis and clinical treatment. Therefore, in this review, we aimed to discuss the current research progress, the potentialfuture clinical applications and the research limitations of three critical classes of ncRNAs in glaucoma, namely microRNAs, long noncoding RNAs, and circular RNAs.

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microRNA Profiling in Glaucoma Eyes With Varying Degrees of Optic Neuropathy by Using Next-Generation Sequencing

Cited by 47 publications

References 63 publications

Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Insights into the regulatory molecules involved in glaucoma pathogenesis

Pathogenesis and prospects for therapeutic clinical application of noncoding RNAs in glaucoma: Systematic perspectives

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