MicroRNA profiling identifies MiR-195 suppresses osteosarcoma cell metastasis by targeting CCND1

Han, Kang; Chen, Xiang; Bian, Na; Ma, Baoan; Yang, Tao; Cai, Chengkui; Fan, Qingyu; Zhou, Yong; Zhao, Ting

doi:10.18632/oncotarget.3560

Cited by 80 publications

(78 citation statements)

References 32 publications

(39 reference statements)

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“…Recent studies showed that miRNAs play vital roles in regulating individual development, cell proliferation, metabolism, differentiation, invasion and migration in different disease [16-22]. A number of studies dispalyed that different miRNAs could increase or decrease cell apoptosis in OA, such as miR-149 and miR-30b [23, 24].…”

Section: Introductionmentioning

confidence: 99%

MiR-1246 Promotes LPS-Induced Inflammatory Injury in Chondrogenic Cells ATDC5 by Targeting HNF4γ

Zhang

Wang

et al. 2017

Cell Physiol Biochem

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Background/Aims: Osteoarthritis (OA) is a common inflammatory joint disease. miRNAs are associated with OA and functionally implicated in the pathogenesis of the disease. In the present study, we investigated the role of miR-1246 in the lipopolysaccharide (LPS)-induced inflammatory injury of ATDC5 cells. Methods: ATDC5 cells were cultured and treated with LPS in a series of concentration (0, 1, 5, and 10 µg/ml) for 5 h. The cells were transfected with miR-1246-mimic, inhibitor, si-HNF4γ or negative control, then were assessed for cell viability using CCK8 assay, apoptosis by flow-cytometry and expressions of miR-1246 and pro-inflammatory cytokines by qRT-PCR and western blot analysis. Results: Cell viability was significantly reduced and cell apoptosis was added in ATDC5 cells injured with LPS at the dosage of 5 and 10 µg/ml. Relative mRNA expressions of pro-inflammatory cytokines (IL-1β, IL-6, IL-8 and TNF-α) were significantly increased. miR-1246 was up-regulated in ATDC5 cells treated with LPS. Moreover, miR-1246 overexpression aggravated LPS-induced decrease in cell viability, increase in apoptosis and overproduction of pro-inflammatory factors. mRNA and protein expressions of HNF4γ were significantly suppressed in cells transfected with miR-124-mimic. Further, miR-1246 knockdown alleviated LPS-induced inflammatory injury by up-regulating the expression of HNF4γ and activation of PI3K/AKT and JAK/STAT pathways. Conclusions: Suppression of miR-1246 alleviated LPS-induced inflammatory injury in chondrogenic ADTC5 cells by up-regulation of HNF4γ and activation of PI3K/AKT and JAK/STAT pathways. The findings of this study will provide a novel viewpoint regarding miR-1246 target for clinical.

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Section: Introductionmentioning

confidence: 99%

MiR-1246 Promotes LPS-Induced Inflammatory Injury in Chondrogenic Cells ATDC5 by Targeting HNF4γ

Zhang

Wang

et al. 2017

Cell Physiol Biochem

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“…For example, Sun et al 26 reported that miR-195 was down-regulated in esophageal squamous cell carcinoma and negatively correlates with advanced clinical stage and lymphatic metastasis. Han et al 27 showed that in osteosarcoma, miR-195 was significantly down-regulated in highly metastatic osteosarcoma cell line and inhibited the invasion and migration of osteosarcoma cells via targeting CCND1. Yang et al 28 showed that miR-195 can inhibit the invasion and metastasis by inhibiting the expression of Bcl-2.…”

Section: Discussionmentioning

confidence: 99%

MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3

Zhou¹,

Han²,

Zhou³

et al. 2016

Int J Gynecol Cancer

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“…Despite the recent advances in the treatment of osteosarcoma such as radiation therapy, adjuvant chemotherapy and surgery that have been achieved, the relative 5-year survival rate of osteosarcoma patients remains discontent [6][7][8][9][10]. Although various studies have investigated the development and mechanism of osteosarcoma, the molecular mechanism of osteosarcoma is still elusive [11][12][13]. Therefore, it is urgent to find novel therapeutic approaches and targets for treating osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1

Wang¹,

Peng²,

Gong³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Increasing evidences suggest that dysregulated expression of miRNAs contributes to the progression of various tumors. However, the underlying function of miR-423-5p in osteosarcoma remains unexplored. Methods: The expression of miR-423-5p and STMN1 were determined in osteosarcoma samples and cell lines via quantitative real-time PCR. Colony formation and Cell Counting Kit-8 (CCK-8) assays were performed to measure cell proliferation ability and transwell analysis was used to detect cell invasion, and dual luciferase reporter assay was perform to analysis the interaction between the miR-423-5p and STMN1. Results: The expression levels of miR-423-5p and STMN1 in the osteosarcoma tissues and cell lines were measured by qRT-PCR. Cell viability was determined using the clone formation and CCK-8 assays. A dual-luciferase reporter and Western blot were performed to stdudy the target gene of miR-423-5p. Here, we showed that miR-423-5p expression was downregulated in osteosarcoma tissues and cell lines. However, the expression of stathmin1 (STMN1) was downregulated in osteosarcoma tissues and cell lines. Moreover, STMN1 expression level was negatively correlated with the miR-423-5p expression in the osteosarcoma tissues. We identified STMN1 was a direct target gene of miR-423-5p in osteosarcoma cell. Overexpression of miR-423-5p inhibited osteosarcoma cell proliferation, colony formation and invasion. Furthermore, we demonstrated that STMN1 was involved in miR-423-5p-mediated cell behavior such as cell proliferation, colony formation and invasion in the osteosarcoma cell. Conclusion: Our present study indicated that miR-423-5p acted as a tumor suppressor gene in osteosarcoma partly through inhibiting STMN1 expression.

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MicroRNA profiling identifies MiR-195 suppresses osteosarcoma cell metastasis by targeting CCND1

Cited by 80 publications

References 32 publications

MiR-1246 Promotes LPS-Induced Inflammatory Injury in Chondrogenic Cells ATDC5 by Targeting HNF4γ

MiR-1246 Promotes LPS-Induced Inflammatory Injury in Chondrogenic Cells ATDC5 by Targeting HNF4γ

MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3

miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1

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