MicroRNA profiling associated with muscle growth in modern broilers compared to an unselected chicken breed

Khatri, Bhuwan; Seo, Dongwon; Shouse, Stephanie; Pan, Jeong Hoon; Hudson, Nicholas J.; Kim, Jae Kyeom; Bottje, Walter; Kong, Byung-Whi

doi:10.1186/s12864-018-5061-7

Cited by 29 publications

(24 citation statements)

References 47 publications

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“…Furthermore, interest in this transcription factor goes beyond stress. For example, when a comparison of a single male modern broiler line compared to a foundational Barred Plymouth Rock chicken line was conducted, it showed that Nrf2 expression in breast muscle was much higher in the modern broiler compared to the foundational BPR line [299], and it was suggested Nrf2-mediated oxidative stress response pathways are involved in breast muscle growth in chickens [300]. In recent years, several compounds, including carnitine, have been shown to have inhibitory activities against multiple components of the NF-κB activation pathway.…”

Section: Other Nutrients and Probioticsmentioning

confidence: 99%

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

et al. 2019

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Poultry in commercial settings are exposed to a range of stressors. A growing body of information clearly indicates that excess ROS/RNS production and oxidative stress are major detrimental consequences of the most common commercial stressors in poultry production. During evolution, antioxidant defence systems were developed in poultry to survive in an oxygenated atmosphere. They include a complex network of internally synthesised (e.g., antioxidant enzymes, (glutathione) GSH, (coenzyme Q) CoQ) and externally supplied (vitamin E, carotenoids, etc.) antioxidants. In fact, all antioxidants in the body work cooperatively as a team to maintain optimal redox balance in the cell/body. This balance is a key element in providing the necessary conditions for cell signalling, a vital process for regulation of the expression of various genes, stress adaptation and homeostasis maintenance in the body. Since ROS/RNS are considered to be important signalling molecules, their concentration is strictly regulated by the antioxidant defence network in conjunction with various transcription factors and vitagenes. In fact, activation of vitagenes via such transcription factors as Nrf2 leads to an additional synthesis of an array of protective molecules which can deal with increased ROS/RNS production. Therefore, it is a challenging task to develop a system of optimal antioxidant supplementation to help growing/productive birds maintain effective antioxidant defences and redox balance in the body. On the one hand, antioxidants, such as vitamin E, or minerals (e.g., Se, Mn, Cu and Zn) are a compulsory part of the commercial pre-mixes for poultry, and, in most cases, are adequate to meet the physiological requirements in these elements. On the other hand, due to the aforementioned commercially relevant stressors, there is a need for additional support for the antioxidant system in poultry. This new direction in improving antioxidant defences for poultry in stress conditions is related to an opportunity to activate a range of vitagenes (via Nrf2-related mechanisms: superoxide dismutase, SOD; heme oxygenase-1, HO-1; GSH and thioredoxin, or other mechanisms: Heat shock protein (HSP)/heat shock factor (HSP), sirtuins, etc.) to maximise internal AO protection and redox balance maintenance. Therefore, the development of vitagene-regulating nutritional supplements is on the agenda of many commercial companies worldwide.

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Section: Other Nutrients and Probioticsmentioning

confidence: 99%

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

et al. 2019

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“…skeletal muscle differentiation and development [10][11][12] . Recent studies have shown that some muscle-specific miRNAs play an important role in the regulation of muscle fiber type specification and maintenance in certain vertebrate species 13,14 .…”

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confidence: 99%

miRNA–mRNA network regulation in the skeletal muscle fiber phenotype of chickens revealed by integrated analysis of miRNAome and transcriptome

Liu

Zhang

Shan

et al. 2020

Sci Rep

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Skeletal muscle fibers are primarily categorized into oxidative and glycolytic fibers, and the ratios of different myofiber types are important factors in determining livestock meat quality. However, the molecular mechanism for determining muscle fiber types in chickens was hardly understood. In this study, we used RNA sequencing to systematically compare mRNA and microRNA transcriptomes of the oxidative muscle sartorius (SART) and glycolytic muscle pectoralis major (PMM) of Chinese Qingyuan partridge chickens. Among the 44,705 identified mRNAs in the two types of muscles, 3,457 exhibited significantly different expression patterns, including 2,364 up-regulated and 1,093 downregulated mRNAs in the SART. A total of 698 chicken miRNAs were identified, including 189 novel miRNAs, among which 67 differentially expressed miRNAs containing 42 up-regulated and 25 downregulated miRNAs in the SART were identified. Furthermore, function enrichment showed that the differentially expressed mRNAs and miRNAs were involved in energy metabolism, muscle contraction, and calcium, peroxisome proliferator-activated receptor (PPAR), insulin and adipocytokine signaling. Using miRNA-mRNA integrated analysis, we identified several candidate miRNA-gene pairs that might affect muscle fiber performance, viz, gga-miR-499-5p/SOX6 and gga-miR-196-5p/CALM1, which were supported by target validation using the dual-luciferase reporter system. This study revealed a mass of candidate genes and miRNAs involved in muscle fiber type determination, which might help understand the molecular mechanism underlying meat quality traits in chickens. Improving meat quality has long been a goal of broiler breeding programs, especially for Chinese native breeds 1,2. However, meat quality is difficult to define because it is a complex trait influenced by numerous factors 3. As the main tissue determining meat quality, skeletal muscle is a heterogeneous tissue composed of different types of muscle fibers, varying in their biochemical and structural characteristics. Previous studies have found that different types of muscle fibers can influence meat quality traits, including meat color, tenderness, water-holding capacity, juiciness, and flavor 4,5. In chickens, myofiber can be divided into red and white fibers, which are referred to as oxidative (type I and IIA) and glycolytic fibers (type IIB), respectively. Oxidative fibers exhibit slow contractility and oxidative metabolism based on mitochondrial oxidative phosphorylation, whereas glycolytic fibers have fast contractility and glycolytic metabolism 6,7. Although the differences between various muscle fiber types in physiology and functionality have been well studied, the molecular regulation of their specification and maintenance in chickens remains largely unknown 8,9. miRNAs are highly conserved non-coding small RNAs that regulate gene expression at the post-transcriptional level in most biological processes. Emerging evidence has demonstrated that miRNAs are involved in

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“…Since this phenotype is similar to the sarcopenia phenotype, the authors speculate that miR-133a might have a significant role in maintaining skeletal muscle mitochondrial functionality. Other miRNAs, such as miR-340-5p and miR-206, have also been shown to regulate ROS generation in skeletal muscle via Nrf2, which is a key factor in regulating redox homeostasis, although the molecular mechanisms involved in its effect in the onset of sarcopenia are still unknown [ 153 , 154 ].…”

Section: Nutrition-dependent Microrna Regulation Of Mitochondrial mentioning

confidence: 99%

Nutrition and microRNAs: Novel Insights to Fight Sarcopenia

et al. 2020

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Sarcopenia is a progressive age-related loss of skeletal muscle mass and strength, which may result in increased physical frailty and a higher risk of adverse events. Low-grade systemic inflammation, loss of muscle protein homeostasis, mitochondrial dysfunction, and reduced number and function of satellite cells seem to be the key points for the induction of muscle wasting, contributing to the pathophysiological mechanisms of sarcopenia. While a range of genetic, hormonal, and environmental factors has been reported to contribute to the onset of sarcopenia, dietary interventions targeting protein or antioxidant intake may have a positive effect in increasing muscle mass and strength, regulating protein homeostasis, oxidative reaction, and cell autophagy, thus providing a cellular lifespan extension. MicroRNAs (miRNAs) are endogenous small non-coding RNAs, which control gene expression in different tissues. In skeletal muscle, a range of miRNAs, named myomiRNAs, are involved in many physiological processes, such as growth, development, and maintenance of muscle mass and function. This review aims to present and to discuss some of the most relevant molecular mechanisms related to the pathophysiological effect of sarcopenia. Besides, we explored the role of nutrition as a possible way to counteract the loss of muscle mass and function associated with ageing, with special attention paid to nutrient-dependent miRNAs regulation. This review will provide important information to better understand sarcopenia and, thus, to facilitate research and therapeutic strategies to counteract the pathophysiological effect of ageing.

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MicroRNA profiling associated with muscle growth in modern broilers compared to an unselected chicken breed

Cited by 29 publications

References 47 publications

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

miRNA–mRNA network regulation in the skeletal muscle fiber phenotype of chickens revealed by integrated analysis of miRNAome and transcriptome

Nutrition and microRNAs: Novel Insights to Fight Sarcopenia

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