MicroRNA profiling and bioinformatics analyses reveal the potential roles of microRNAs in chordoma

Chen, Kangwu; Chen, Hao; Zhang, Kai; Sun, Shuang; Mo, Jianqiang; Lü, Jian; Zhou, Qian; Yang, Huilin

doi:10.3892/ol.2017.6839

Cited by 12 publications

(11 citation statements)

References 25 publications

(25 reference statements)

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“…Jäger et al [ 22 ] suggest that the differential expression of HOX genes in chordomas of the clivus and sacrum suggests an oncofetal mechanism in gene regulation linked to the anatomic site. In the study from Chen et al, [ 13 ] human (hsa-) miR-185-5p was identified as a crucial miRNA in chordoma development via the Wnt signaling pathway. Wen et al [ 11 ] indicate that Sam68 is an independent prognostic factor for the local recurrence-free survival of sacral chordomas [hazard ratio = 5.929, 95% confidence interval (95% CI): 1.092–32.188, P = .039].…”

Section: Discussionmentioning

confidence: 99%

A giant lumbar chordoma

Zhou

Hu²,

Wu³

et al. 2018

Medicine

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Rationale:Chordomas are malignant neoplasms derived from incomplete regression of notochordal tissue along the craniococcygeal axis.It is rare for Chordoma arising from the lumbar spine and the traditional long-term prognosis is typically poor.Patient concerns:The persistent pain in the left side of the waist about 2 years.Diagnoses:Chordoma.Interventions:The patient was treated with surgical resection of the total tumor, followed by the spinal internal fixation of L1 to L2 with pedicle screws.Outcomes:After 5 month follow-up,we find the recurrence in the original lesion.At the 15 month follow-up,the patient was dead after a lot of times revisit by various doctor.Lessons:So It is suggest that the diagnosis should be carried out accurately at the early stage, the lesions and source of lesions should be cut away as broadly as possible, also the radiation and chemotherapy should be carried out after the operation as necessary.

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Section: Discussionmentioning

confidence: 99%

A giant lumbar chordoma

Zhou

Hu²,

Wu³

et al. 2018

Medicine

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“…On the basis of the previous microarray profiling results in our research 19 , 24 , the independent online tools miRanda (Website: http://www.microrna.org/microrna/home.do ) was used to predict the binding site of NONHSAT024778 targeting miR-1290. TargetScan v7.2 (Website: http://www.targetscan.org/vert_72 ), microRNAorg (Website: http://www.microrna.org/microrna/home.do ), and PITA (Website: http://www.genie.weizmann.ac.il/index.html ) were utilized to determine the target gene Robo1 and miR-1290 binding site of Robo1 and predict the possible target genes of NONHSAT024778-miR-1290.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study used miRNA microarray profiling to reveal the different miRNA expression levels between chordoma and fetal nucleus pulposus (FNP) and found that miR-1290 is the most down-regulated miRNA within chromosome 1 19 . Chromosome 1 gene deletion or amplification is the most common chromosome variation in chordoma 20 - 22 .…”

Section: Introductionmentioning

confidence: 99%

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LncRNA-NONHSAT024778 promote the proliferation and invasion of chordoma cell by regulating miR-1290/Robo1 axis

et al. 2021

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Chordoma is a malignant bone tumor originating from the embryonic remnants of the notochord. lncRNAs act as competing endogenous RNAs (ceRNAs) and play a critical role in tumor pathology. However, the biological role of lncRNA-NONHSAT024778 and the underlying molecular mechanism in chordoma remains unknown. qRT-PCR was used to analyze the expression changes of NONHSAT024778 and miR-1290 in chordoma tissues and cell lines. Bioinformatics analysis and luciferase reporter assay were applied to detect the targeting binding effect between NONHSAT024778 and miR-1290, and between Robo1 and miR-1290. The effect of NONHSAT024778 on chordoma cell proliferation and invasion and its regulation of miR-1290 by acting as a ceRNA were also investigated. An increased NONHSAT024778 expression was correlated with a decreased miR-1290 level in chordoma tissues. NONHSAT024778 knockdown suppressed the proliferation and invasion of chordoma cells. miR-1290 restored expression rescued the carcinogenic function of NONHSAT024778. Bioinformatics analysis showed that NONHSAT024778 acted as ceRNA to regulate Robo1 via sponging miR-1290 in chordoma cells, thereby promoting chordoma cell malignant progression. In vivo results confirmed the anti-tumor effects of NONHSAT024778 knockdown activating miR-1290 to inhibit the oncogene Robo1. NONHSAT024778 is substantially overexpressed, whereas miR-1290 is decreased in chordoma tissue. NONHSAT024778-miR-1290-Robo1 axis plays a critical role in chordoma tumorigenesis and might be a potential predictive biomarker for the diagnosis and therapeutic target among patients with chordoma.

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“…org/mamm_31/) and microRNA.org databases (http://www. mirbase.org/) (16,17). Binding sites between miR-524-5p and the target genes were searched to identify potential interactions between them.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway

et al. 2021

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Preeclampsia is a pregnancy disorder that is primarily associated with maternal and neonatal or fetal morbidity and mortality. The discovery of dysregulated microRNAs (miRs) and their roles in preeclampsia has provided new insight into the mechanisms involved in pregnancy-related disorders. In the present study, quantitative PCR demonstrated that the expression levels of miR-524-5p were lower in patients with preeclampsia than those in normal pregnant women. Cell Counting Kit-8 and Transwell assays indicated that overexpression of miR-524-5p promoted the proliferation and invasion of HTR-8/SVneo cells, whereas inhibition of miR-524-5p suppressed HTR-8/SVneo cell proliferation and invasion. Furthermore, NUMB endocytic adaptor protein (NUMB), a negative regulator of the Notch signaling pathway and a target gene of miR-524-5p, limited the effects of miR-524-5p on HTR-8/SVneo cell invasion and migration. The present study demonstrated that miR-524-5p regulated the proliferation and invasion of HTR-8/SVneo cells at least partly by targeting NUMB to regulate the Notch signaling pathway.

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MicroRNA profiling and bioinformatics analyses reveal the potential roles of microRNAs in chordoma

Cited by 12 publications

References 25 publications

A giant lumbar chordoma

A giant lumbar chordoma

LncRNA-NONHSAT024778 promote the proliferation and invasion of chordoma cell by regulating miR-1290/Robo1 axis

MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway

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