MicroRNA Omics Analysis of Camellia sinesis Pollen Tubes in Response to Low-Temperature and Nitric Oxide

Xu, Xiaohan; Wang, Weidong; Sun, Yi; Xing, Anqi; Wu, Zichen; Tian, Zhiqiang; Li, Xuyan; Wang, Yuhua

doi:10.3390/biom11070930

Cited by 6 publications

(2 citation statements)

References 81 publications

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“…Oxidative stress and inflammation are thought to be the groundwork of liver disease caused by factors including heavy metal accumulation, viral inflammation, alcohol, drugs, and other aspects [ 39 ]. Consistently, the current study revealed that Cd could cause oxidative stress and inflammatory damage in hepatocytes.…”

Section: Discussionmentioning

confidence: 99%

Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes

Cao

Yang

Lin

et al. 2022

IJMS

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Cadmium (Cd) is a toxic heavy metal that can accumulate in the liver of animals, damaging liver function. Inflammation and oxidative stress are considered primary causes of Cd-induced liver damage. Selenium (Se) is an antioxidant and can resist the detrimental impacts of Cd on the liver. To elucidate the antagonism of Se on Cd against hepatocyte injury and its mechanism, duck embryo hepatocytes were treated with Cd (4 μM) and/or Se (0.4 μM) for 24 h. Then, the hepatocyte viability, oxidative stress and inflammatory status were assessed. The findings manifested that the accumulation of reactive oxygen species (ROS) and the levels of pro-inflammatory factors were elevated in the Cd group. Simultaneously, immunofluorescence staining revealed that the interaction between NOD-like receptor pyran domain containing 3 (NLRP3) and apoptosis-associated speck-like protein (ASC) was enhanced, the movement of high-mobility group box 1 (HMGB1) from nucleus to cytoplasm was increased and the inflammatory response was further amplified. Nevertheless, the addition of Se relieved the above-mentioned effects, thereby alleviating cellular oxidative stress and inflammation. Collectively, the results suggested that Se could mitigate Cd-stimulated oxidative stress and inflammation in hepatocytes, which might be correlated with the NLRP3 inflammasome and HMGB1/nuclear factor-κB (NF-κB) signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes

Cao

Yang

Lin

et al. 2022

IJMS

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“…With the development and popularization of omics technology, an increasing number of studies focusing on pollen grain function, including transcriptomics (Keller et al, 2018 ), proteomics (Chaturvedi et al, 2016 ; Keller et al, 2018 ), and metabolomics (Fragallah et al, 2018 ; Paupière et al, 2019 ), have been conducted in recent years. With the application of transcriptomics technology, Xu et al ( 2021 ) found that the abnormal growth of the Clonorchis sinensis pollen tube caused by NO-induced by low temperature could be due to the differential expression of miR5721, miR5819, and miR1429-3p, and novel_mir_18 regulated their target genes related to pollen tube growth, resulting in changes in insufficient energy, cell wall organization, and ion distribution for pollen tube growth, thus inhibiting polar growth of C. sinensis pollen tube tips after low-temperature treatment. Fragallah et al ( 2018 ) focused on different germination abilities from two clones of Chinese fir [ Cunningham ia lanceolata (Lamb.)…”

Section: Introductionmentioning

confidence: 99%

Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)

Dai

Jiang

Zhao³

et al. 2022

Front. Plant Sci.

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Cross-pollination can improve the percentage of fruit set and fruit weight for most red flesh varieties in pitaya. The technology of pollen storage was very important for successful cross-pollination. However, till present, the technology of pollen storage is unsatisfactory in pitaya production. In this study, pitaya pollen stored at low temperature was taken as the research object, and its physicochemical indexes, metabolomics, and transcriptomics were studied. The results showed that in vitro pollen germination rate decreased significantly with the increase in storage time. Soluble sugar and soluble protein content of pollen peaked on the first day of storage, whereas its relative conductivity, and manlondialdehyde (MDA) and proline contents increased gradually during storage. At the same time, the antioxidant enzyme system of pollen was also affected. Superoxide dismutase (SOD) activity decreased, while the activities of catalase (CAT) and peroxidase (POD) increased and superoxide anion generation rate increased gradually during storage. According to the metabolomics results, amino acid, peptide, nucleotide, plant hormone, terpene, alcohol, phenol, flavonoid, sterol, vitamin, ester, sphingolipid, and ketone contents increased significantly during storage, whereas flavonoid and pigment contents declined gradually. During pollen storage, the gene expressions related to carbohydrate metabolism, protein metabolism, acid and lipid metabolism, sterol metabolism, plant hormone metabolism, and signal transductions were significantly downregulated. With KEGG pathway analysis, isoquinoline alkaloid biosynthesis, tyrosine metabolism, alanine, aspartate, and glutamate metabolism of pollen were affected significantly during low-temperature storage. Correlation analysis showed that the gene expression patterns of HuRP2, HuUPL1, and HuAAT2 had significant effects on pollen germination. D-arabinose 5-phosphate and myricetin were positively correlated with pollen germination rate, which was valuable for studying preservation agents. In this study, the changes in pollen during low-temperature storage were described from the level of metabolites and genes, which could provide theoretical support for the research and development of pollen long-term storage technology in pitaya.

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Integrative small RNA and transcriptome analysis provides insight into key role of miR408 towards drought tolerance response in cowpea

2021

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Cowpea, a highly nutritious legume crop exhibits high resilience to heat and drought stress. Small RNA and transcriptome sequencing was performed in an elite high-yield Indian cultivar, Pusa Komal. Overall, 233 conserved and 10 novel drought-responsive miRNAs, and around 2995/497 drought-responsive genes were observed in libraries generated from leaf/root tissues of cowpea. Using 5' RLM-RACE approach, NAC29, auxin response factor 8, copper chaperone for superoxide dismutase, scarecrow-like protein 6, mitochondrial substrate carrier family protein B, and laccase 12 were identi ed as cleaved targets of differentially expressed miRNAs such as miR167, miR171m, miR398c, miR408, miR1514, novel_792, and novel_796 in cowpea. Moreover, the potential role of differentially up-regulated miR408 in cowpea was exploited by the generation of transgenic cowpea lines overexpressing amiR408a-3p. The transgenic OX-amiR408 cowpea lines displayed enhanced drought and salinity tolerance as compared to control plants and maintained higher relative water content, chlorophyll, and proline content, and lower cellular H2O2 content under drought stress. The SEM analysis revealed reduced stomatal aperture and higher trichome density in transgenic OX-amiR408 lines.Furthermore, genes related to trichome development, transparent testa glabra 1 (TTG1), and glabrous in orescence stems (GIS3) showed ~1.45fold change in transgenic lines. Selected DGE for GO enriched terms for biological processes like lipid metabolic processes, hydrogen peroxide catabolic process, and response to oxidative stress, such as LAC3, LAC12, GDSL esterase/lipase CPRD49 and peroxidase 25 were altered in transgenic OX-amiR408 lines. Key MessageOxidative stress response, stomatal, trichome density and alteration in lignin metabolism as preliminary drought response in transgenic OX-amiR408 cowpea lines

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MicroRNA Omics Analysis of Camellia sinesis Pollen Tubes in Response to Low-Temperature and Nitric Oxide

Cited by 6 publications

References 81 publications

Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes

Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes

Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)

Integrative small RNA and transcriptome analysis provides insight into key role of miR408 towards drought tolerance response in cowpea

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