MicroRNA of N‐region from SARS‐CoV‐2: Potential sensing components for biosensor development

Abstract

An oligonucleotide DNA probe has been developed for the application in the DNA electrochemical biosensor for the early diagnosis of coronavirus disease (COVID‐19). Here, the virus microRNA from the N‐gene of severe acute respiratory syndrome‐2 (SARS‐CoV‐2) was used for the first time as a specific target for detecting the virus and became a framework for developing the complementary DNA probe. The sequence analysis of the virus microRNA was carried out using bioinformatics tools including basic local alignment search tools, multiple sequence alignment from CLUSTLW, microRNA database (miRbase), microRNA target database, and gene analysis. Cross‐validation of distinct strains of coronavirus and human microRNA sequences was completed to validate the percentage of identical and consent regions. The percent identity parameter from the bioinformatics tools revealed the virus microRNAs’ sequence has a 100% match with the genome of SARS‐CoV‐2 compared with other coronavirus strains, hence improving the selectivity of the complementary DNA probe. The 30 mer with 53.0% GC content of complementary DNA probe 5′ GCC TGA GTT GAG TCA GCA CTG CTC ATG GAT 3′ was designed and could be used as a bioreceptor for the biosensor development in the clinical and environmental diagnosis of COVID‐19.

Inactivation of SARS-CoV-2 by charged particles for Future Vaccine Production Applications: A Monte Carlo study

“…This is a typical radius obtained from the literature ( Shereen et al, 2020 ). It should be noted that the diameter of SARS-CoV-2 ranges between 50 and 200 nm ( Halim et al, 2021 ). Two PDB files with correspondent IDs of 6VXX and 6VYO were employed to construct spike and RNA structures, respectively ( Mariano et al, 2020 ).…”

Advancing COVID-19 Detection: High-Performance RNA Biosensing via Electrical Interactions

“…As a response to these challenges, researchers have undertaken a mission to create advanced diagnostic technologies that combine affordability, portability, rapid detection, and ease of use, making them suitable for widespread adoption within healthcare systems [3], [4]. This effort aligns with the changing landscape of medical research, where fields like biotechnology and electronics are converging to develop state-of-the-art solutions [5], [6]. Consequently, a new approach has emerged electrical-based biosensors for detecting COVID-19 [7]- [9].…”

Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor‐based gold nanoparticles

“…Our probe has a higher binding affinity when immobilized on the functionalized surface electrode, especially on AuNPs. Besides AuNPs, carbon quantum dots (CDs) can also be a conductivity enhancer linked with great chemical linkers such as 3‐aminopropyl triethoxysilane (APTES) due to their abundant hydroxyl group 9 . The reduced oxide layer on graphene is also vital with linker attachment due to their oxide layer availability 10 .…”

“…Besides AuNPs, carbon quantum dots (CDs) can also be a conductivity enhancer linked with great chemical linkers such as 3-aminopropyl triethoxysilane (APTES) due to their abundant hydroxyl group. 9 The reduced oxide layer on graphene is also vital with linker attachment due to their oxide layer availability. 10 As far as we are concerned, virus DNA is very suitable as a biomarker as it can directly detect the presence of HPV and identify multitudinous types of high-risk HPV.…”