MicroRNA molecules as predictive biomarkers of adaptive responses to strength training and physical inactivity in haemodialysis patients

Abstract

The miRNA-206 and miRNA-23a play an important role in muscle tissue hypertrophy, regeneration and atrophy. Both of these miRNAs have been highlighted as promising adaptation predictors; however, the available evidence on associations is inconclusive. Therefore, our aim was to assess the expression levels of these two miRNAs as predictors of change in muscle function during strength training and physical inactivity among dialysed patients. For this purpose, 46 haemodialysis patients were monitored for 12-weeks of either intradialytic strength training (EXG, n = 20) or physical inactivity during dialysis (CON, n = 26). In both groups of patients, we assessed the baseline expression levels of miRNA-23a and miRNA-206 and the isometric force generated during hip flexion (HF) contraction before and after the 12-week period. Among the EXG group, the expression of miRNA-206 predicted the change in HF (R2 = 0.63, p = 0.0005) much more strongly than the expression of miRNA-23a (R2 = 0.21, p = 0.027). Interestingly, baseline miRNA-23a (R2 = 0.30, p = 0.006) predicted the change in HF much more than miRNA-206 (p = ns) among the CON group. Our study indicates that the baseline expression of miRNA-206 could predict the response to strength training, while miRNA-23a could serve as a potential predictive marker of functional changes during physical inactivity in dialysis patients.

Effects of intradialytic exercise for advanced-age patients undergoing hemodialysis: A randomized controlled trial

“…A renal rehabilitation guideline established in 2019 recommended exercise therapy for patients undergoing hemodialysis at an evidence level of 1b [10]. Exercise therapy has been reported to improve muscle tissue adaptation by promoting physiological processes such as myogenesis, skeletal muscle regeneration, and regulation of myostatin mRNA activity [11]. The combination of aerobic exercise and resistance training as exercise therapy during dialysis leads to greater improvement in exercise tolerance than a single type of exercise [12].…”

Age and sex differences in the effectiveness of intradialytic resistance training on muscle function

“…Intradialytic resistance training (IRT) positively affected physical functions, mobility, nutritional status, body composition, quality of life, dialysis-related clinical outcomes and mortality in CKD-5D patients 8 – 12 . IRT improved muscle functions (MF) of lower extremities, positively affects survival in CKD-5D patients, and the change in MF was associated with the presence of diabetes mellitus and microribonucleic acid expression profiles as detailed in our previous studies 13 – 16 . Besides clinical efficiency in the prevention of physical function decline, large inter-individual differences in the physiological response to IRT have been reported among CKD5-D patients 17 – 19 .…”

Selected In Situ Hybridization Methods: Principles and Application

“…The main role of ribonucleic acid contains the encoding and decoding of genetic information, the ability to form different types of RNAs participating in gene expression, and gene regulation [37,38]. Many methods used for the visualization and imaging of each RNA type in cells and tissues are based on the understanding of cell-to-cell communication and on the understanding of the limitations and principles of synthesis and critical functions of RNAs in organisms, such as 3 -end polyadenylation in RNA stability and cancer development [37,39].…”