MicroRNA miR394 regulates flowering time in Arabidopsis thaliana

Bernardi, Yanel; Ponso, María Agustina; Belén, Federico; Vegetti, Abelardo Carlos; Dotto, Marcela C.

doi:10.1007/s00299-022-02863-0

Cited by 12 publications

(11 citation statements)

References 87 publications

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“…Other significantly differentially expressed conserved miRNAs identified in this study included miR394 and miR396, both of which were also differentially regulated between juvenile and mature tissue types in avocado, mango and macadamia [16]. MiR394 regulates processes in the shoot apical meristem (SAM) [27] and regulates flowering time in Arabidopsis thaliana [28]. The miR396 family repress expression of the transcription factor class GROWTH REGULATING FACTOR (GRF), and GRF-INTERACTING FACTOR (GIF) transcriptional co-regulators, and this complex is crucial in many aspects of plant growth and development in angiosperms [29].…”

Section: Discussionmentioning

confidence: 87%

“…The miR156 family was found in all sample types, with the highest read count (696) in JUV samples and lowest (28) in MAT samples. The miR172 family was found in all sample types, but with very low expression levels -in JUV samples the expression value was 1, 7 in MAT samples, 1 in REV samples and 2 in IVM samples.…”

Section: Identification Of Conserved Mirnas In Silver Birchmentioning

confidence: 99%

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Differentially Expressed Conserved Plant Vegetative Phase-change Related Micro RNAs as Markers of Juvenility for Silver Birch In Vitro Propagation

Krivmane¹,

Samsone²,

Ruņģis³

2022

Preprint

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In plants, phase change from the juvenile stage to maturity is a tightly controlled process at the physiological and genetic level, which is controlled by evolutionary highly conserved microRNAs. These phase changes are more pronounced in woody plant species, but the majority of molecular genetic studies on the regulation of this transition has been done in annual model or crop species. This process is of particular significance for the in vitro propagation of woody plant species, as individuals or tissues that have undergone the transition to vegetative maturity are recalcitrant to propagation. Development of effective methodologies for silver birch vegetative propagation are required to increase the efficiency of breeding programs. Conserved miRNAs that were differentially expressed between juvenile and mature silver birch tissues were identified using high-throughput sequencing of small RNA libraries. These differentially expressed miRNAs could potentially be utilized to develop markers indicating the juvenility or maturity of silver birch explants and in vitro cultures. In addition, the obtained results will provide an insight into the molecular mechanisms regulating vegetative phase change in silver birch and other perennial woody plant species.

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Section: Discussionmentioning

confidence: 87%

Section: Identification Of Conserved Mirnas In Silver Birchmentioning

confidence: 99%

Differentially Expressed Conserved Plant Vegetative Phase-change Related Micro RNAs as Markers of Juvenility for Silver Birch In Vitro Propagation

Krivmane¹,

Samsone²,

Ruņģis³

2022

Preprint

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“…First, we tested whether miR394 can promote WUS function when expressed ectopically because previous studies had found that miR394 acted synergistically with WUS only in the shoot meristem, whereas it functions independently of WUS in other tissues (Ni et al ., 2012; Song et al ., 2012, 2013; Bernardi et al ., 2022). To this end, we analyzed well‐established induction of shoot meristems by ectopic WUS function by expressing combinations of MIR394B and WUS in the L er ecotype from the PLETHORA2 ( PLT2 ) promoter, which provides high expression in cells of the root pole of zygotic embryos (Aida et al ., 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Exploring alternative ways how SE formation can be enhanced, we found that miR394 strongly enhances SE formation when expressed ectopically together with WUS. Previous studies found diverse roles of endogenous miR394 in regulating stem cell competence in the shoot meristem, leave morphogenesis, flowering time, and stress response (Song et al ., 2012, 2013; Knauer et al ., 2013; Bernardi et al ., 2022). However, the mechanisms of miR394 functions are largely unknown.…”

Section: Discussionmentioning

confidence: 99%

miR394 enhances WUSCHEL‐induced somatic embryogenesis in Arabidopsis thaliana

Holt

Chen

et al. 2023

New Phytologist

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Summary Many plant species can give rise to embryos from somatic cells after a simple hormone treatment, illustrating the remarkable developmental plasticity of differentiated plant cells. However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro‐RNA394 (miR394) promotes shoot meristem maintenance in Arabidopsis thaliana, but the underlying mechanisms have remained elusive. We analyzed whether miR394 affects indirect somatic embryogenesis and determined the transcriptome of embryogenic callus upon miR394‐enhanced somatic embryogenesis. We show that ectopic miR394 expression enhances somatic embryogenesis in the recalcitrant Ler accession when co‐expressed with the transcription factor WUSCHEL (WUS) and that miR394 acts in this process through silencing the target LEAF CURLING RESPONSIVENESS (LCR). Furthermore, we show that higher endogenous miR394 levels are required for the elevated embryogenic potential of the Columbia accession compared with Ler, providing a mechanistic explanation for this natural variation. Our transcriptional analysis provides a framework for miR394 function in regulating pluripotency by expanding WUS‐mediated direct transcriptional repression.

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“…For instance, several miRNAs have been identified to function in controlling flowering. For example, the miR156, miR172, miR169, miR159, and miR399, as well as their target genes, play a pivotal role in flowering regulation [ 3 , 14 , 15 , 16 ]. By controlling the expression of an AP2 - like gene, miR172 influenced flowering time [ 17 ], floral organ characteristics [ 18 ], and flower certainty [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Sequencing Reveals Novel microRNAs Involved in the Continuous Flowering Trait of Longan (Dimocarpus longan Lour.)

Waheed

Liang

Zhang

et al. 2022

IJMS

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A major determinant of fruit production in longan (Dimocarpus longan Lour.) is the difficulty of blossoming. In this study, high-throughput microRNA sequencing (miRNA-Seq) was carried out to compare differentially expressed miRNAs (DEmiRNAs) and their target genes between a continuous flowering cultivar ‘Sijimi’ (SJ), and a unique cultivar ‘Lidongben’ (LD), which blossoms only once in the season. Over the course of our study, 1662 known miRNAs and 235 novel miRNAs were identified and 13,334 genes were predicted to be the target of 1868 miRNAs. One conserved miRNA and 29 new novel miRNAs were identified as differently expressed; among them, 16 were upregulated and 14 were downregulated. Through the KEGG pathway and cluster analysis of DEmiRNA target genes, three critical regulatory pathways, plant–pathogen interaction, plant hormone signal transduction, and photosynthesis-antenna protein, were discovered to be strongly associated with the continuous flowering trait of the SJ. The integrated correlation analysis of DEmiRNAs and their target mRNAs revealed fourteen important flowering-related genes, including COP1-like, Casein kinase II, and TCP20. These fourteen flowering-related genes were targeted by five miRNAs, which were novel-miR137, novel-miR76, novel-miR101, novel-miR37, and csi-miR3954, suggesting these miRNAs might play vital regulatory roles in flower regulation in longan. Furthermore, novel-miR137 was cloned based on small RNA sequencing data analysis. The pSAK277-miR137 transgenic Arabidopsis plants showed delayed flowering phenotypes. This study provides new insight into molecular regulation mechanisms of longan flowering.

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MicroRNA miR394 regulates flowering time in Arabidopsis thaliana

Cited by 12 publications

References 87 publications

Differentially Expressed Conserved Plant Vegetative Phase-change Related Micro RNAs as Markers of Juvenility for Silver Birch In Vitro Propagation

Differentially Expressed Conserved Plant Vegetative Phase-change Related Micro RNAs as Markers of Juvenility for Silver Birch In Vitro Propagation

miR394 enhances WUSCHEL‐induced somatic embryogenesis in Arabidopsis thaliana

High-Throughput Sequencing Reveals Novel microRNAs Involved in the Continuous Flowering Trait of Longan (Dimocarpus longan Lour.)

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