MicroRNA miR-374, a potential radiosensitizer for carbon ion beam radiotherapy

Abstract

In this study, we compared the microRNA (miRNA) profiles of a control and X-ray- and carbon ion beam-resistant cells to identify miRNAs that can be used as radiosensitizers and biomarkers. Mouse squamous cell carcinoma line NR-S1, its X-ray-resistant derivative X60, and its carbon ion beam‑resistant derivative C30 were subjected to miRNA microarray analysis. Expression of miRNAs shown to be upregulated or downregulated in the microarray analysis was confirmed by qRT-PCR. Downregulated miRNAs were overexpressed in human pancreatic cancer cell lines PANC1 and MIA PaCa-2, and the resulting cells were tested for radiosensitivity using colony-forming and sphere-forming assays. Of 1,265 miRNAs analyzed, 4 were downregulated and 11 were upregulated in X-ray-resistant and carbon ion beam-resistant cells. Two of the downregulated miRNAs, miR-196 and miR-374, were selected for overexpression in PANC1 and MIA PaCa-2 cells. Overexpression of miR-374 sensitized PANC-1 and MIA PaCa-2 cells toward carbon ion beam radiation. miRNA miR-374 has the potential to be a new radiosensitizer for carbon ion beam radiotherapy and a new biomarker to determine the optimal treatment for cancer.

Effects of microRNA-374 on proliferation, migration, invasion, and apoptosis of human SCC cells by targeting Gadd45a through P53 signaling pathway

“…microRNAs (miRs) are vital regulators of many pathological and physiological processes, including metastasis and tumorigenesis [11]. In addition, miR-374 has been suggested to be a novel biomarker for determining the most appropriate treatment for cancer and a novel radiation sensitizer for carbon ion beam radiotherapy [12]. In the present study, we aim to investigate the effects of miR-374 on proliferation, migration, invasion, and apoptosis of human SCC cells by targeting Gadd45a through the P53 signaling pathway.…”

The latest progress on miR‐374 and its functional implications in physiological and pathological processes

“…Meanwhile, miR‐374b overexpression in BxPC3‐R cells recovered cisplatin sensitivity almost to the levels displaying in BxPC3 parental cells. In the same year, Baek et al first screened the change of miRNAs with microarray analysis in mouse squamous cell carcinoma line NR‐S1, X60 cells (established by irradiating NR‐S1 cells with 10 Gy of X‐ray radiation once every 2 weeks) and C30 cells (established by irradiating NR‐S1 cells with 5 Gy of carbon ion beam radiation once every 2 weeks). They also demonstrated that miR‐374c‐5p and miR‐196a‐5p were down‐regulated.…”

Retracted: microRNA‐374 inhibits proliferation and promotes apoptosis of mouse melanoma cells by inactivating the Wnt signalling pathway through its effect on tyrosinase

“…The aberrant expression of microRNA‐374 (miR‐374) has been reported in many types of human tumours, including gastric cancer, lung cancer and oesophageal cancer . Furthermore, miR‐374 has been identified as a novel biomarker in determining the most appropriate treatment option for cancer and a novel radiation sensitizer for carbon ion beam radiotherapy . Tyrosinase (TYR) is a copper‐containing enzyme known for its participation in a variety of biological processes including wound healing, pigment production, exoskeleton fabrication and innate immunity and hardening .…”