MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma

Abstract

Members of the miR-125 family are strongly expressed in several tissues, particularly brain, but may be dysregulated in cancer including adult and pediatric glioma. In this study, miR-125 members were downregulated in pilocytic astrocytoma (PA) as a group compared to non-neoplastic brain in the Agilent platform. In the Nanostring platform, miR-125 members were downregulated primarily in pleomorphic xanthoastrocytomas and gangliogliomas. Using CISH for miR-125b, highest levels of expression were present in grade II tumors (11/33, 33% grade II tumors with 3+ expression compared to 3/70, 4% grade I tumors) (p < 0.001). When focusing on the two histologic subgroups with the largest number of samples, PA and diffuse astrocytoma (DA), the highest expression levels were present in DA, in comparison with the PA group (p = 0.01). Overexpression of miR-125b in pediatric low grade glioma (PLGG) derived cell lines (Res186, Res259, and BT66) resulted in decreased growth and invasion, as well as apoptosis. Additionally, miR-125b overexpression in BT66 resulted in senescence. These findings suggest that miR-125 is frequently underexpressed in PLGG, and overexpression results in a decrease in cell growth and induction of apoptosis, findings that deserve further investigation given its potential as a novel therapeutic strategy for PLGG.

MicroRNA-125b-5p regulates IL-1β induced inflammatory genes via targeting TRAF6-mediated MAPKs and NF-κB signaling in human osteoarthritic chondrocytes

“…Furthermore, we also demonstrated that miRNAs hsa-miR-199a-3p and hsa-miR-140-3p negatively regulate COX-2 and ADAMTS-5 expression, respectively in human OA chondrocytes 14,15 . Recently, the function of miR-125b was discovered in various cell types and its association with several human disorders was reported 16–19 . In OA, the function of miR-125b was somewhat defined by few studies only, in one study miR-125b was reported to regulate aggrecanase-1 expression in human chondrocytes 20 and in another study, miR-125b was reported to regulate the inflammatory activities in stimulated chondrogenic cells via MIP-1α signaling event 21 .…”

MiR-125 inhibited cervical cancer progression by regulating VEGF and PI3K/AKT signaling pathway

“…Also, miR-125 was upregulated in esophageal adenocarcinoma and associated with poor prognosis [24]. However, miR-125 was under-expressed in glioma and regulated cell growth and invasion [25]. Besides, Chen H showed that miR-125 downregulation acted as a potential biomarker for colorectal cancer treatment [26].…”

Modulation of MicroRNAs as a Potential Molecular Mechanism Involved in the Beneficial Actions of Physical Exercise in Alzheimer Disease

“…A single miRNA can regulate different targets, and it is estimated that miRNAs regulate approximately 60% of all human protein coding genes by post-transcriptional mechanisms [ 22 ]. Therefore, miRNAs are involved in several biological processes, including apoptosis [ 23 ], cell metabolism, homeostasis [ 24 ], differentiation [ 25 ], cell cycle [ 26 ], growth [ 27 ], cell survival [ 28 ], cell proliferation, and migration [ 29 ]. In addition, the involvement of miRNAs has also been demonstrated in pathological processes of different diseases, such as heart failure [ 30 ], arterial hypertension [ 31 ], type 2 diabetes mellitus, obesity [ 32 ], Chagas disease cardiomyopathy [ 33 ], and AD [ 34 ].…”