MicroRNA-mediated regulation of T helper cell differentiation and plasticity

Baumjohann, Dirk; Ansel, K. Mark

doi:10.1038/nri3494

Cited by 322 publications

(273 citation statements)

References 139 publications

(164 reference statements)

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“…Although not covered here, it is important to consider that T cell plasticity is regulated by micro-RNA-mediated post-transcriptional regulation of a wide variety of genes -such as those involved in cytokine signalling, TCR and co-stimulatory signalling, cytosolic signalling and transcriptional regulatory pathways 163 . 169 and IL-17-expressing T H 2 cells in atopic asthma 13 .…”

Section: Micrornasmentioning

confidence: 99%

Harnessing the plasticity of CD4+ T cells to treat immune-mediated disease

2016

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| CD4 + T cells differentiate and acquire distinct functions to combat specific pathogens but can also adapt their functions in response to changing circumstances. Although this phenotypic plasticity can be potentially deleterious, driving immune pathology, it also provides important benefits that have led to its evolutionary preservation. Here, we review CD4 + T cell plasticity by examining the molecular mechanisms that regulate it -from the extracellular cues that initiate and drive cells towards varying phenotypes, to the cytosolic signalling cascades that decipher these cues and transmit them into the cell and to the nucleus, where these signals imprint specific gene expression programmes. By understanding how this functional flexibility is achieved, we may open doors to new therapeutic approaches that harness this property of T cells.

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Section: Micrornasmentioning

confidence: 99%

Harnessing the plasticity of CD4+ T cells to treat immune-mediated disease

2016

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“…Recently, numerous studies regarding the role of miRNAs in development and function of immune cells have emerged, and several excellent reviews suggest that miRNAs represent a novel layer of control over cellular physiology [1][2][3]. T cells that express the CD8 coreceptor and recognize peptide-MHC class I complexes have a key role in the control of numerous viral or intracellular bacterial infections [4].…”

mentioning

confidence: 99%

microRNAs function in CD8+T cell biology

Liang

Pan

2015

Journal of Leukocyte Biology

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“…Helper T cells play an essential role in regulating the immune response, and a great deal of effort has been undertaken to understand their development and function (illustrated in several recent reviews [1][2][3] ). However, many questions remain, and many approaches have been utilized to study the mechanisms controlling helper T cell development and function.…”

Section: Introductionmentioning

confidence: 99%

“…In addition miRNAs are important later on in the development of many tissues. They are thought to function by fine-tuning the expression of genes required for developmental programs 1 . In helper T cells miRNAs play multiple roles and are required for regulatory T cell (Treg) development [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Singh

Garden

Läng

et al. 2016

JoVE

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Helper T cell development and function must be tightly regulated to induce an appropriate immune response that eliminates specific pathogens yet prevents autoimmunity. Many approaches involving different model organisms have been utilized to understand the mechanisms controlling helper T cell development and function. However, studies using mouse models have proven to be highly informative due to the availability of genetic, cellular, and biochemical systems. One genetic approach in mice used by many labs involves retroviral transduction of primary helper T cells. This is a powerful approach due to its relative ease, making it accessible to almost any laboratory with basic skills in molecular biology and immunology. Therefore, multiple genes in wild type or mutant forms can readily be tested for function in helper T cells to understand their importance and mechanisms of action. We have optimized this approach and describe here the protocols for production of high titer retroviruses, isolation of primary murine helper T cells, and their transduction by retroviruses and differentiation toward the different helper subsets. Finally, the use of this approach is described in uncovering mechanisms utilized by microRNAs (miRNAs) to regulate pathways controlling helper T cell development and function.

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MicroRNA-mediated regulation of T helper cell differentiation and plasticity

Cited by 322 publications

References 139 publications

Harnessing the plasticity of CD4+ T cells to treat immune-mediated disease

Harnessing the plasticity of CD4+ T cells to treat immune-mediated disease

microRNAs function in CD8+T cell biology

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

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