MicroRNA-mediated regulation of differentiation and trans-differentiation in stem cells

Ong, Sang Ging; Lee, Won Hee; Kodo, Kazuki; Wu, Joseph C.

doi:10.1016/j.addr.2015.04.004

Cited by 59 publications

(40 citation statements)

References 171 publications

(183 reference statements)

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“…MicroRNAs are small (20–26 nucleotides in length) noncoding RNAs, which have been shown to exist widely in plants and animals (Valencia‐Sanchez, Liu, Hannon, & Parker, ; John et al, ). MicroRNAs can participate in many important biological processes, including proliferation, migration, invasion, and angiogenesis (Ong, Lee, Kodo, & Wu, ; Ronald, ). To date, multiple studies have shown that miR‐210 can function as a tumor suppressor by inhibiting cell proliferation and promoting apoptosis, such as in ovarian cancer (Giannakakis et al, ) and in esophageal squamous cell carcinoma (Tsuchiya et al, ).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐210 regulates human trophoblast cell line HTR‐8/SVneo function by attenuating Notch1 expression: Implications for the role of microRNA‐210 in pre‐eclampsia

Wang

Liu

et al. 2019

Molecular Reproduction Devel

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Successful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA‐210‐3p (miR‐210), which is increased in the placenta of pre‐eclampsia. Furthermore, miR‐210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre‐eclampsia. Previous studies have demonstrated that miR‐210 regulates HUVEC (human umbilical vein endothelial cell)‐mediated angiogenesis by regulating the NOTCH1 signaling pathway. Studies by our group have previously identified that NOTCH1 plays a positive role in regulating trophoblast functions. However, the miR‐210/NOTCH1 signaling pathway in the regulation of trophoblasts and pre‐eclampsia has not been characterized. Therefore, this study was conducted to investigate the role of miR‐210 and its relationship with NOTCH1 in trophoblasts. We first examined the expression levels of miR‐210 and NOTCH1 in pre‐eclamptic and normals placentas. Next, the expression and location of miR‐210 and NOTCH1 in the first‐trimester villi, maternal decidua, and placenta of late pregnancy were shown via in situ hybridization and immunohistochemistry. The trophoblast cell line HTR‐8/SVneo was used to investigate the effects of miR‐210 on the expression of NOTCH1 and cell bioactivity by upregulation and downregulation strategies. The results showed that miR‐210 expression was increased, whereas NOTCH1 expression was decreased in pre‐eclamptic placenta compared with controls. Upregulation of miR‐210 decreased NOTCH1 expression, impaired HTR‐8/SVneo proliferation, migration, invasion, and tube‐like formation capabilities, and promoted apoptosis. In contrast, downregulation of miR‐210 resulted in the opposite effects. These findings suggested that miR‐210 might act as a contributor to trophoblast dysfunction by attenuating NOTCH1 expression.

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Section: Introductionmentioning

confidence: 99%

MicroRNA‐210 regulates human trophoblast cell line HTR‐8/SVneo function by attenuating Notch1 expression: Implications for the role of microRNA‐210 in pre‐eclampsia

Wang

Liu

et al. 2019

Molecular Reproduction Devel

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“…miRNAs are present in animals and plants and have been detected in several human fluids (16). They are critical in many biological processes, including the regulation of cell differentiation, proliferation, apoptosis, angiogenesis, and metabolism (17). Specific plasma-related miRNAs were identified in maternal plasma or serum in normal pregnancies, as well as in pathological pregnancy conditions, such as PE (18,19).…”

Section: Introductionmentioning

confidence: 99%

Dysregulated circulating miRNAs in preeclampsia

et al. 2016

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Abstract. Preeclampsia (PE) is a pregnancy-related disease with potentially severe consequences with respect to foeto-maternal morbidity and mortality. However, the molecular pathogenesis of PE remains largely unknown. Recent reports have shown that microRNAs (miRNAs or miRs) may play important roles in the development of PE. Analysing the miRNAs in sera from preeclamptic women may improve our understanding of the pathophysiological mechanisms of the disease. The aim of this retrospective study was to identify whether circulating miRNAs were differentially expressed in PE patients compared with controls. Serum samples from 23 women who developed PE were compared with samples from 44 pregnant controls. Seventeen circulating miRNAs previously described in PE were chosen for evaluation of their expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR). In the maternal serum, the miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p levels were found to be significantly higher in the PE patients than in the controls (P<0.05). Using a logistic regression model, we evaluated the discriminant power of those differentially expressed miRNAs, and the combination of miR-210-5p and miR-574-5p yielded an area under the curve of 0.7223 for discriminating PE patients from the controls. In conclusion, the fact that four circulating miRNAs (miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p) were differentially expressed in the sera of women who developed PE compared with controls confirms the possible pathophysiological role of miRNAs in PE.

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“…Certainly, a single miRNA does not absolutely determine cell fate specification, and emerging evidence suggests that specific miRNAs have the potential to be involved in the transition from one cell type to another [50,51].. Our results suggested that miR26a could trigger MSC differentiation toward ECs by targeting GSK-3β, and GSK-3β-p21 signaling regulated EC differentiation (Fig. 1).…”

Section: Discussionmentioning

confidence: 51%

The microRNA-dependent cell fate of multipotent stromal cells differentiating to endothelial cells

Cha

Choi

Lee

et al. 2016

Experimental Cell Research

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MicroRNA-mediated regulation of differentiation and trans-differentiation in stem cells

Cited by 59 publications

References 171 publications

MicroRNA‐210 regulates human trophoblast cell line HTR‐8/SVneo function by attenuating Notch1 expression: Implications for the role of microRNA‐210 in pre‐eclampsia

MicroRNA‐210 regulates human trophoblast cell line HTR‐8/SVneo function by attenuating Notch1 expression: Implications for the role of microRNA‐210 in pre‐eclampsia

Dysregulated circulating miRNAs in preeclampsia

The microRNA-dependent cell fate of multipotent stromal cells differentiating to endothelial cells

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