MicroRNA-mediated bioengineering for climate-resilience in crops

Patil, Suraj; Joshi, Shrushti; Jamla, Monica; Zhou, Xianrong; Taherzadeh, Mohammad J.; Suprasanna, Penna; Kumar, Vinay

doi:10.1080/21655979.2021.1997244

Cited by 8 publications

(2 citation statements)

References 197 publications

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“…The miRNAs (microRNAs) are ncRNAs (noncoding RNAs) developed from the individual-strand hairpin RNAs predecessor. These showed expressions by attaching a corresponding gene sequence surrounded by targeting mRNAs [ 48 , 49 ]. Several studies reported that miRNAs were involved in different stress and developmental activities [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis and Expression Profiling of DUF668 Genes in Glycine max under Salt Stress

Zaynab

Sharif

et al. 2023

Plants

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The DUF668 gene performs a critical role in mitigating the impact of abiotic stress factors. In this study, we identified 30 DUF668 genes in a soybean genome, distributed across fifteen chromosomes. The phylogenetic analysis classified the DUF668 genes into three groups (group I, group II, and group III). Interestingly, gene structure analysis illustrated that several GmDUF668 genes were without introns. Furthermore, the subcellular localization results suggested that GmDUF668 proteins were present in the nucleus, mitochondria, cytoplasm, and plasma membrane. GmDUF668 promoters were analyzed in silico to gain insight into the presence of regulatory sequences for TFs binding. The expression profiling illustrated that GmDUF668 genes showed expression in leaves, roots, nodules, and flowers. To investigate their response to salt stress, we utilized the RNA sequencing data of GmDUF668 genes. The results unveiled that GmDUF668-8, GmDUF668-20, and GmDUF668-30 genes were upregulated against salt stress treatment. We further validated these findings using qRT-PCR analysis. These findings provide a scientific basis to explore the functions of GmDUF668 genes against different stress conditions.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis and Expression Profiling of DUF668 Genes in Glycine max under Salt Stress

Zaynab

Sharif

et al. 2023

Plants

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“…MicroRNAs (miRNAs) are a group of tiny-non-coding RNAs formed from individual-strand hairpin RNA precursors. These miRNAs switch gene expression by attaching to corresponding sequences surrounded by target mRNAs (Jamla et al, 2021;Patil et al, 2021). Extensive progress has been put together in finding the targets of peanut miRNAs that contribute to various stresses and developmental activities (Zhao et al, 2010(Zhao et al, , 2015Chi et al, 2011;Zhang et al, 2017;Figueredo et al, 2020;Tong et al, 2021).…”

Section: Microrna: Emerging Players For Crop Improvement and Stress T...mentioning

confidence: 99%

Genome-Wide Characterization of Ascorbate Peroxidase Gene Family in Peanut (Arachis hypogea L.) Revealed Their Crucial Role in Growth and Multiple Stress Tolerance

et al. 2022

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Ascorbate peroxidase (APX), an important antioxidant enzyme, plays a significant role in ROS scavenging by catalyzing the decrease of hydrogen peroxide under various environmental stresses. Nevertheless, information about the APX gene family and their evolutionary and functional attributes in peanut (Arachis hypogea L.) was not reported. Therefore, a comprehensive genome-wide study was performed to discover the APX genes in cultivated peanut genome. This study identified 166 AhAPX genes in the peanut genome, classified into 11 main groups. The gene duplication analysis showed that AhAPX genes had experienced segmental duplications and purifying selection pressure. Gene structure and motif investigation indicated that most of the AhAPX genes exhibited a comparatively well-preserved exon-intron pattern and motif configuration contained by the identical group. We discovered five phytohormones-, six abiotic stress-, and five growth and development-related cis-elements in the promoter regions of AhAPX. Fourteen putative ah-miRNAs from 12 families were identified, targeting 33 AhAPX genes. Furthermore, we identified 3,257 transcription factors from 38 families (including AP2, ARF, B3, bHLH, bZIP, ERF, MYB, NAC, WRKY, etc.) in 162 AhAPX genes. Gene ontology and KEGG enrichment analysis confirm the role of AhAPX genes in oxidoreductase activity, catalytic activity, cell junction, cellular response to stimulus and detoxification, biosynthesis of metabolites, and phenylpropanoid metabolism. Based on transcriptome datasets, some genes such as AhAPX4/7/17/77/82/86/130/133 and AhAPX160 showed significantly higher expression in diverse tissues/organs, i.e., flower, leaf, stem, roots, peg, testa, and cotyledon. Likewise, only a few genes, including AhAPX4/17/19/55/59/82/101/102/137 and AhAPX140, were significantly upregulated under abiotic (drought and cold), and phytohormones (ethylene, abscisic acid, paclobutrazol, brassinolide, and salicylic acid) treatments. qRT-PCR-based expression profiling presented the parallel expression trends as generated from transcriptome datasets. Our discoveries gave new visions into the evolution of APX genes and provided a base for further functional examinations of the AhAPX genes in peanut breeding programs.

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Zinc Toxicity and Tolerance in Plants

Shamsi,

Zhang,

et al. 2023

Heavy Metal Toxicity and Tolerance in Plants

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MicroRNA-mediated bioengineering for climate-resilience in crops

Cited by 8 publications

References 197 publications

Genome-Wide Analysis and Expression Profiling of DUF668 Genes in Glycine max under Salt Stress

Genome-Wide Analysis and Expression Profiling of DUF668 Genes in Glycine max under Salt Stress

Genome-Wide Characterization of Ascorbate Peroxidase Gene Family in Peanut (Arachis hypogea L.) Revealed Their Crucial Role in Growth and Multiple Stress Tolerance

Zinc Toxicity and Tolerance in Plants

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