MicroRNA let-7-5p targets the juvenile hormone primary response gene Krüppel homolog 1 and regulates reproductive diapause in Galeruca daurica

Duan, Tian-Feng; Gao, Shandian; Wang, Haichao; Li, Ling; Li, Yanyan; Tan, Yao; Pang, Bao-Ping

doi:10.1016/j.ibmb.2022.103727

Cited by 14 publications

(16 citation statements)

References 56 publications

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“…53 Notably, it was also found to be involved in oogenesis, reproductive diapause or reproduction by targeting the juvenile hormone (JH) earlyresponse gene Krüppel-homolog 1. 29,54,55 miR-2779 was identified as an ovary-biased miRNA in Acipenser schrenckii, 56 but our results showed that it was expressed in all tissues sampled and was abundant in the head of B. dorsalis. Additionally, we found that miR-34 was upregulated during sexual maturation in the fat body of B. dorsalis, indicating that it was possibly involved in reproductive development.…”

Section: Discussionmentioning

confidence: 58%

“…Our previous study revealed that it was essential for insect metamorphosis by targeting the ecdysone signaling pathway E75 gene in B. dorsalis 53 . Notably, it was also found to be involved in oogenesis, reproductive diapause or reproduction by targeting the juvenile hormone (JH) early‐response gene Krüppel‐homolog 1 29,54,55 . miR‐2779 was identified as an ovary‐biased miRNA in Acipenser schrenckii , 56 but our results showed that it was expressed in all tissues sampled and was abundant in the head of B. dorsalis .…”

Section: Discussionmentioning

confidence: 67%

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RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

Zhang

et al. 2022

Pest Management Science

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Background The oriental fruit fly, Bactrocera dorsalis, is a highly invasive pest in East Asia and the Pacific. With the development of pesticides resistance, environment‐friendly pesticides are urgently needed. MicroRNAs (miRNAs) are critical regulators of numerous biological processes, including reproduction. Thus, it is significant to identify reproductive‐related miRNAs in this notorious pest to facilitate its control, such as RNAi‐based biopesticides targeting essential miRNAs. Results A high‐throughput sequencing was carried out to identify miRNAs involved in reproduction from the ovary and fat body at four developmental stages [1 day (d), 5, 9, and 13 days post‐eclosion] in female B. dorsalis. Results showed that 98 and 74 miRNAs were differentially expressed in ovary and fat body, respectively, during sexual maturation. Gene ontology analysis showed that target genes involved in oogenesis and lipid particle accounted for 33% and 15% of the total targets, respectively. Among these differentially expressed miRNAs, we found by qPCR that miR‐311‐3p was enriched in the ovary and down‐regulated during sexual maturation. Injection of agomir‐miR‐311‐3p resulted in arrested ovarian development, reduced egg deposition and progeny viability. Endophilin B1 was confirmed to be the target of miR‐311‐3p, via dual‐luciferase assay and expression profiling. Knockdown of Endophilin B1 resulted in reproductive defects similar to those caused by injection of miR‐311‐3p agomir. Thus, miR‐311‐3p might play a critical role in female reproduction by targeting Endophilin B1. Conclusion Our data not only provides knowledge on the abundance of reproductive‐related miRNAs and target genes, but also promotes new control strategies for this pest. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 67%

RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

Zhang

et al. 2022

Pest Management Science

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“…However, less identical DEMs have been found in these studies, including miR‐1‐3p between A. albopictus and S. bullata , and miR‐277‐3p among A. albopictus , S. bullata , and G. daurica . Moreover, at present, only 1 miRNA, let‐7‐5p , has been confirmed experimentally to play an important role in the regulation of insect diapause (Duan et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we identified 222 microRNAs at different diapausal stages in G. daurica adults, and we found that miR‐2765‐3p was mostly downregulated, up to over 32‐fold, after adults entered into diapause (Duan et al ., 2021). More recently, we have verified that microRNA let‐7‐5p regulates reproductive diapause in G. daurica by targeting Kr‐h1 (Duan et al ., 2022). These studies provide a foundation for elucidating the function of miRNAs in the regulation of reproductive diapause in G. daurica .…”

Section: Introductionmentioning

confidence: 99%

MicroRNA miR‐2765‐3p regulates reproductive diapause by targeting FoxO in Galeruca daurica

Duan

Wang

et al. 2022

Insect Science

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The forkhead box O (FoxO), as a conserved transcription factor, plays an indispensable role in regulating insect diapause. However, how FoxO is regulated to control diapause in insects remains unknown. In this study, we discovered functional binding sites for miR-2765-3p in the 3 untranslated region of FoxO in Galeruca daurica. The luciferase reporter assay showed that miR-2765-3p targeted FoxO and suppressed its expression. The expression profiles of miR-2765-3p and FoxO displayed opposite patterns during the female developmental process. Overexpression of miR-2765-3p by the injection of the miR-2765-3p agomir into adult females reduced FoxO expression, leading to the suppression of lipid accumulation, promotion of ovarian development, and inhibition of reproductive diapause. This is similar to the phenotype that results from the depletion of FoxO by injecting dsFoxO into adult females. In addition, the repression of miR-2765-3p by injecting the miR-2765-3p antagomir increased the FoxO transcript level, leading to the stimulation of lipid accumulation, depression of ovarian development, and induction of reproductive diapause. A hormone injection assay showed that the juvenile hormone (JH) agonist (methoprene) upregulated miR-2765-3p and downregulated FoxO. Notably, injecting methoprene rescued ovarian development defects associated with miR-2765-3p inhibition. These findings indicate that the JH/miR-2765-3p/FoxO axis plays a vital role in the regulation of reproductive diapause in G. daurica.

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“…Galeruca daurica (Coleoptera: Chrysomelidae), a serious grassland pest since its sudden outbreaks on the Inner Mongolia grasslands in 2009 ( Zhou et al, 2016 , Zhou et al, 2019 ), has continually spread and caused great losses to pasture on the Inner Mongolia grasslands ( Ma et al, 2019 , Ma et al, 2021 ), and levels of damage has increased year by year ( Duan et al, 2022 ). The application of traditional chemical insecticides to control outbreaking pests in desert steppes has the advantages of a good insecticidal effect with rapid results, especially organophosphorus, pyrethroids, chloronicotinyl insecticide ( Dong et al, 2016 ; Gao et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)

Ren

Zhang²,

Ni³

et al. 2022

Front. Physiol.

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In recent years, the leaf beetle Galeruca daurica has broken out in the northern grasslands of Inner Mongolia, its management still mainly depends on chemical control using traditional insecticides or with novel action. The study was aim to identify mutation locus associated with resistance to diamide insecticides in field population of G. daurica, to provide a reference for rational selection of insecticides and to avoid the rapid resistance development to diamide insecticides. We cloned the full length of the ryanodine receptor gene of G. daurica (GdRyR), constructed 3D model and transmembrane regions by homologous modeling based on deduced amino acid sequence. Two potential mutation loci (Gly4911Glu and Ile4754Met) and allelic mutation frequencies were detected in individuals of G. daurica. In addition, their binding patterns to two diamide insecticides (chlorantraniliprole, cyantraniliprole) were analyzed separately using a molecular docking method. The full-length cDNA sequence of GdRyR (GenBank accession number: OP828593) was obtained by splicing and assembling, which is 15,399 bp in length and encodes 5,133 amino acids. The amino acid similarity of GdRyR with that of other Coleopteran insects were 86.70%–91.33%, which possessed the typical structural characteristics. An individual resistance allelic mutation frequency test on fifty field leaf beetles has identified 12% and 32% heterozygous individuals at two potential mutation loci Gly4911Glu and Ile4754Met, respectively. The affinity of the I4754M mutant model of GdRyR for chlorantraniliprole and cyantraniliprole was not significantly different from that of the wild type, and all had non-covalent interactions such as hydrogen bonding, hydrophobic interactions and π-cation interactions. However, the G4911E mutant model showed reduced affinity and reduced mode of action with two diamide insecticides, thus affecting the binding stability of the ryanodine receptor to the diamide insecticides. In conclusion, the G4911E mutation in GdRyR may be a potential mechanism for the development of resistance to diamide insecticides on G. daurica and should be a key concern for resistance risk assessment and reasonable applications of diamide insecticides for control in future. Moreover, this study could provide a reference for ryanodine receptor structure-based insecticides design.

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MicroRNA let-7-5p targets the juvenile hormone primary response gene Krüppel homolog 1 and regulates reproductive diapause in Galeruca daurica

Cited by 14 publications

References 56 publications

RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

MicroRNA miR‐2765‐3p regulates reproductive diapause by targeting FoxO in Galeruca daurica

Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)

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