MicroRNA, hsa-miR-200c, is an independent prognostic factor in pancreatic cancer and its upregulation inhibits pancreatic cancer invasion but increases cell proliferation

Abstract

BackgroundRecently, the microRNA-200 family was reported to affect cancer biology by regulating epithelial to mesenchymal transition (EMT). Especially, the expression of miR-200c has been shown to be associated with upregulating the expression of E-cadherin, a gene known to be involved in pancreatic cancer behavior. However, the significance of miR-200c in pancreatic cancer is unknown.MethodsIn the present study, we investigated the relationship between E-cadherin and miR-200c expression in a panel of 14 pancreatic cancer cell lines and in macro-dissected formalin-fixed paraffin-embedded (FFPE) tissue samples obtained from 99 patients who underwent pancreatectomy for pancreatic cancer. We also investigated the effects of miR-200c on the proliferation and invasion of pancreatic cancer cells.ResultsWe found that patients with high levels of miR-200c expression had significantly better survival rates than those with low levels of miR-200c expression. We also found a remarkably strong correlation between the levels of miR-200c and E-cadherin expression.ConclusionsThese data indicate that miR-200c may play a role in the pancreatic cancer biology and may be a novel marker for the prognosis of pancreatic cancer.

miR-200c Targets CDK2 and Suppresses Tumorigenesis in Renal Cell Carcinoma

“…Ectopic expression of miR-200c precursors resulted in enhanced cell proliferation but reduced invasion and migration behaviors in colorectal cancer cell lines (37). Upregulation of miR-200c inhibited invasion but increased cell proliferation in pancreatic cancer (38). Therefore, depending on which factors are essential to drive tumorigenesis in the specific cellular milieu, the same miRNA may play different roles in different tumors.…”

How to Explain the Contradiction of microRNA 200c Expression and Survival in Solid Tumors?: a Meta-analysis

“…Like this article, other three studies estimated the prognostic value of miRNA 200c expression in tumor tissue (Yu et al, 2010;Marchini et al, 2011;Karaayvaz et al, 2012). Among these studies, two studies reported HR and its 95% CI (Marchini et al, 2011;Toiyama et al, 2013) and the rest ones were processed by Matthew Sydes and Jayne Tierney's method to acquire survival data (Tierney et al, 2007;Marchini et al, 2011;Karaayvaz et al, 2012).…”

Regulation of Colorectal Carcinoma Stemness, Growth, and Metastasis by an miR-200c-Sox2–Negative Feedback Loop Mechanism

“…These contrary findings may be due, in part, to the different source organization and empirical method models. With regard to the role of miR-200c in tumor growth, several recent studies have reported its conflicting roles (23,(38)(39)(40).…”