MicroRNA expression profiling reveals potential roles for microRNA in the liver during pigeon (Columba livia) development

Wang, Xun; Yan, Ping; Liu, Lei; Luo, Yi; Zhao, Ling; Liu, Haifeng; Tang, Qianzi; Long, Keren; Jin, Long; Ma, Jideng; Jiang, Anan; Li, Xuewei; Li, Mingzhou

doi:10.1016/j.psj.2020.09.039

Cited by 8 publications

(9 citation statements)

References 42 publications

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“…As an important organ of the animal body, the liver is closely related to the endocrine and metabolic levels during growth and development [31]. Studies have found that the liver has multiple functions in the body, such as the secretion of bile and detoxification [1].…”

Section: Discussionmentioning

confidence: 99%

Developmental Changes of Duckling Liver and Isolation of Primary Hepatocytes

Bao

Wang

et al. 2023

Animals

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The liver is the main site of fat synthesis and plays an important role in the study of fat deposition in poultry. In this study, we investigated the developmental changes of duckling livers and isolated primary duck hepatocytes. Firstly, we observed morphological changes in duckling livers from the embryonic period to the first week after hatching. Liver weight increased with age. Hematoxylin-eosin and Oil Red O staining analyses showed that hepatic lipids increased gradually during the embryonic period and declined post-hatching. Liver samples were collected from 21-day-old duck embryos for hepatocyte isolation. The hepatocytes showed limited self-renewal and proliferative ability and were maintained in culture for up to 7 days. Typical parenchymal morphology, with a characteristic polygonal shape, appeared after two days of culture. Periodic acid-Schiff (PAS) staining analysis confirmed the characteristics of duck embryo hepatocytes. PCR analysis showed that these cells from duck embryos expressed the liver cell markers ALB and CD36. Immunohistochemical staining and immunofluorescence analysis also confirmed ALB and CK18 expression. Our findings provide a novel insight regarding in vitro cell culture and the characteristics of hepatocytes from avian species, which could enable further studies concerning specific research on duck lipid metabolism.

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Section: Discussionmentioning

confidence: 99%

Developmental Changes of Duckling Liver and Isolation of Primary Hepatocytes

Bao

Wang

et al. 2023

Animals

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“…The STEM algorithm was used to cluster the expression profile of DEmiRNAs in pigeon skeletal muscle growth and development [ 14 ]. The STEM clustering tool assigned each miRNA according to its time expression characteristics.…”

Section: Resultsmentioning

confidence: 99%

“…The STEM algorithm is widely used to cluster expression data sampled in a time sequence and analyze its expression pattern [ 13 , 14 ]. We performed cluster analysis on DEmiRNAs using the STEM algorithm.…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNA–mRNA Networks Associated with Pigeon Skeletal Muscle Development and Growth

Ding

Chen

Tao

et al. 2022

Animals

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The growth and development of skeletal muscle determine the productivity of pigeon meat production, and miRNA plays an important role in the growth and development of this type of muscle. However, there are few reports regarding miRNA regulating the growth and development of skeletal muscle in pigeons. To explore the function of miRNA in regulating the growth and development of pigeon skeletal muscle, we used RNA sequencing technology to study the transcriptome of pigeons at two embryonic stages (E8 and E13) and two growth stages (D1 and D10). A total of 32,527 mRNAs were identified in pigeon skeletal muscles, including 14,378 novel mRNAs and 18,149 known mRNAs. A total of 2362 miRNAs were identified, including 1758 known miRNAs and 624 novel miRNAs. In total, 839 differentially expressed miRNAs (DEmiRNAs) and 11,311 differentially expressed mRNAs (DEGs) were identified. STEM clustering analysis assigned DEmiRNAs to 20 profiles, of which 7 were significantly enriched (p-value < 0.05). These seven significantly enriched profiles can be classified into two categories. The first category represents DEmiRNAs continuously downregulated from the developmental stage to the growth stage of pigeon skeletal muscle, and the second category represents DEmiRNAs with low expression at the development and early growth stage, and significant upregulation at the high growth stage. We then constructed an miRNA–mRNA network based on target relationships between DEmiRNAs and DEGs belonging to the seven significantly enriched profiles. Based on the connectivity degree, 20 hub miRNAs responsible for pigeon skeletal muscle development and growth were identified, including cli-miR-20b-5p, miR-130-y, cli-miR-106-5p, cli-miR-181b-5p, miR-1-z, cli-miR-1a-3p, miR-23-y, cli-miR-30d-5p, miR-1-y, etc. The hub miRNAs involved in the miRNA–mRNA regulatory networks and their expression patterns during the development and growth of pigeon skeletal muscle were visualized. GO and KEGG enrichment analysis found potential biological processes and pathways related to muscle growth and development. Our findings expand the knowledge of miRNA expression in pigeons and provide a database for further investigation of the miRNA–mRNA regulatory mechanism underlying pigeon skeletal muscle development and growth.

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“…Thus far, miRNA expression has been studied in the liver tissues of buffalos ( Rha and Gyeol Yoo, 2015 ), dairy cows ( Bu et al, 2017 ), mice ( Seclaman et al, 2019 ), rats ( Wang et al, 2017 ), pigeons ( Wang et al, 2020 ), pigs ( Kai et al, 2019 ), chickens ( Xu et al, 2019 ), and geese ( Zheng et al, 2015 ). RNA-Seq was used to construct 41 pairs of ceRNA networks on liver tissue from three Holstein cows, which provide new insight into resolving bovine lipid metabolism ( Liang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

MiRNA-Seq reveals key MicroRNAs involved in fat metabolism of sheep liver

Fei

Jin²,

Yuan³

et al. 2023

Front. Genet.

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There is a genetic difference between Hu sheep (short/fat-tailed sheep) and Tibetan sheep (short/thin-tailed sheep) in tail type, because of fat metabolism. Previous studies have mainly focused directly on sheep tail fat, which is not the main organ of fat metabolism. The function of miRNAs in sheep liver fat metabolism has not been thoroughly elucidated. In this study, miRNA-Seq was used to identify miRNAs in the liver tissue of three Hu sheep (short/fat-tailed sheep) and three Tibetan sheep (short/thin-tailed sheep) to characterize the differences in fat metabolism of sheep. In our study, Hu sheep was in a control group, we identified 11 differentially expressed miRNAs (DE miRNAs), including six up-regulated miRNAs and five down-regulated miRNAs. Miranda and RNAhybrid were used to predict the target genes of DE miRNAs, obtaining 3,404 target genes. A total of 115 and 67 GO terms as well as 54 and 5 KEGG pathways were significantly (padj < 0.05) enriched for predicted 3,109 target genes of up-regulated and 295 target genes of down-regulated miRNAs, respectively. oar-miR-432 was one of the most up-regulated miRNAs between Hu sheep and Tibetan sheep. And SIRT1 is one of the potential target genes of oar-miR-432. Furthermore, functional validation using the dual-luciferase reporter assay indicated that the up-regulated miRNA; oar-miR-432 potentially targeted sirtuin 1 (SIRT1) expression. Then, the oar-miR-432 mimic transfected into preadipocytes resulted in inhibited expression of SIRT1. This is the first time reported that the expression of SIRT1 gene was regulated by oar-miR-432 in fat metabolism of sheep liver. These results could provide a meaningful theoretical basis for studying the fat metabolism of sheep.

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MicroRNA expression profiling reveals potential roles for microRNA in the liver during pigeon (Columba livia) development

Cited by 8 publications

References 42 publications

Developmental Changes of Duckling Liver and Isolation of Primary Hepatocytes

Developmental Changes of Duckling Liver and Isolation of Primary Hepatocytes

Identification of miRNA–mRNA Networks Associated with Pigeon Skeletal Muscle Development and Growth

MiRNA-Seq reveals key MicroRNAs involved in fat metabolism of sheep liver

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