MicroRNA expression profiling of thymic epithelial tumors

Ganci, Federica; Vico, Carmen; Korita, Etleva; Sacconi, Andrea; Gallo, Enzo; Mori, Federica; Cambria, A; Russo, Emanuele; Anile, Marco; Vitolo, Domenico; Pescarmona, Edoardo; Blandino, R.; Facciolo, Francesco; Venuta, Federico; Blandino, Giovanni; Marino, Mirella; Fazi, Francesco

doi:10.1016/j.lungcan.2014.04.008

Cited by 43 publications

(52 citation statements)

References 38 publications

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“…10 By using 2 independent retrospective cohorts of formalinfixed paraffin-embedded (FFPE) tissue, in a previous work, we identified a group of miRNAs differentially expressed between thymic tumors and normal thymic tissues. 11 Among the 14 most significantly deregulated miRNAs (9 up-and 5 down-regulated) we observed the down-regulation of miRNAs with tumor-suppressor function, as miR-145-5p, and the up-regulation of onco-miRNAs, as miR-21-5p and miR-148a-3p and miR-141-3p, in TET, in line with what previously reported for other tumors. 11,12 Based on this evidence, we have collected blood plasma from TET patients at the time of surgery and during followup (with 35 months as median of time from surgery), and from healthy donors samples, to evaluate expression levels of the up-regulated miRNAs identified in the tumor.…”

Section: Introductionsupporting

confidence: 90%

“…11 Among the 14 most significantly deregulated miRNAs (9 up-and 5 down-regulated) we observed the down-regulation of miRNAs with tumor-suppressor function, as miR-145-5p, and the up-regulation of onco-miRNAs, as miR-21-5p and miR-148a-3p and miR-141-3p, in TET, in line with what previously reported for other tumors. 11,12 Based on this evidence, we have collected blood plasma from TET patients at the time of surgery and during followup (with 35 months as median of time from surgery), and from healthy donors samples, to evaluate expression levels of the up-regulated miRNAs identified in the tumor. We hereby report that the expression levels of specific onco-miRNAs, that we found upregulated in the blood plasma collected from TET patients at surgery compared to healthy donors samples resulted significantly reduced in follow-up samples.…”

Section: Introductionsupporting

confidence: 90%

“…Among the up-regulated miRNAs previously identified in FFPE tumor tissues 11 we select 6 miRNAs (miR-21-5p, miR-148a-3p, miR-141-3p, miR-34b-5p, miR-34c-5p, miR-455-5p) to evaluate their expression in blood plasma and peripheral blood mononuclear cells collected from TET patients at the time of surgery and during follow-up (with 35 months as median of time from surgery), and from healthy donors samples. The characteristics of TET patient specimens are reported in the Patients and Methods section and summarized in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…1A). As internal control we selected miR-940, which resulted unchanged in tumor versus normal FFPE tissue samples in our previous work by array method 11 (Fig. S1A).…”

Section: Resultsmentioning

confidence: 99%

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Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

Bellissimo

Russo

Ganci³

et al. 2015

Cancer Biology & Therapy

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Thymic epithelial cells give rise to both thymoma and thymic carcinoma. A crucial advance in thymic epithelial tumors (TET) management may derive from the identification of novel molecular biomarkers able to improve diagnosis, prognosis and treatment planning.In a previous study, we identified microRNAs that were differentially expressed in tumor vs normal thymic tissues. Among the microRNAs resulted upregulated in TET tissues, we evaluated miR-21-5p, miR-148a-3p, miR-141-3p, miR-34b-5p, miR-34c-5p, miR-455-5p as blood plasma circulating non-invasive biomarkers for TET management.We firstly report that the expression levels of specific onco-miRNAs, that we found upregulated in the blood plasma collected from TET patients at surgery, resulted significantly reduced in follow-up samples.This pilot study suggests that circulating miR-21-5p and miR-148a-3p could represent novel non-invasive biomarkers to evaluate the efficacy of therapy and the prognosis of TET.

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Section: Introductionsupporting

confidence: 90%

Section: Introductionsupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

“…1A). As internal control we selected miR-940, which resulted unchanged in tumor versus normal FFPE tissue samples in our previous work by array method 11 (Fig. S1A).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

Bellissimo

Russo

Ganci³

et al. 2015

Cancer Biology & Therapy

Self Cite

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“…The abnormal expression of an individual miRNA may have a marked influence on cellular physiology, potentially leading to disease, including cancer (14 demonstrated that miRNAs are involved in carcinogenesis and disease progression of a number of types of human cancer, and that they regulate diverse biological processes, including cell proliferation, the cell cycle, differentiation, apoptosis, migration, invasion, metastasis and sensitivity to chemotherapy and radiation (15,16). It is reported that numerous miRNAs are deregulated in cancer tissue when compared with normal tissue (17)(18)(19), acting either as tumor suppressors or oncogenes depending on the functions of their target genes (20,21). Therefore, further investigation of the expression and molecular mechanisms of miRNAs in PDAC is likely to provide therapeutic targets for patients with PDAC.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑216b reduces growth, migration and invasion of pancreatic ductal adenocarcinoma cells by directly targeting ρ‑associated coiled‑coil containing protein kinase 1

et al. 2018

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Abstract. Developments in cancer therapy have greatly improved the survival time for patients with pancreatic ductal adenocarcinoma (PDAC); however, the prognosis of patients with PDAC remains poor. Understanding the expression patterns and functions of microRNAs may provide strategies for the diagnosis and treatment of patients with PDAC. The present study aimed to explore the expression and functions of microRNA-216b (miR-216b) in PDAC. The expression of miR-216b in PDAC tissues and cell lines was quantified with reverse transcription-quantitative polymerase chain reaction. An miR-216b mimic was introduced into PDAC cells to induce the effects of miR-21b overexpression. The effects of miR-216b overexpression on growth, migration and invasion of PDAC cells were evaluated by cell proliferation assay, migration and invasion assays, respectively. The molecular mechanism underlying the suppressive effects of miR-216b on PDAC was also examined; a direct target gene of miR-216b, ρ-associated coiled-coil containing protein kinase 1 (ROCK1), was downregulated by ROCK1 short interfering RNA to investigate the effects on growth, migration and invasion of PDAC cells. The present study revealed that miR-216b was significantly downregulated in PDAC tissues and cell lines. Overexpression of miR-216b inhibited growth, migration and invasion of PDAC cells in vitro. ROCK1 was identified as a direct target gene of miR-216b in pancreatic cancer and the downregulation of ROCK1 resembled the effects of miR-216b overexpression in PDAC cells. Taken together, miR-216b acted as a tumor suppressor in PDAC and may represent a novel therapeutic target in PDAC.

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MicroRNA‐410 functions as a tumor suppressor by targeting angiotensin II type 1 receptor in pancreatic cancer

Guo

Zhang

et al. 2015

IUBMB Life

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MicroRNAs (miRNAs) act as key regulators of gene expression in diverse biological processes and are intimately involved in tumorigenesis. However, the underlying molecular mechanisms of miR-410 in pancreatic cancer remain poorly understood. In this study, we found that miR-410 overexpression suppressed pancreatic cancer cell growth in vitro and in vivo as well as cell invasion and migration. miR-410 also resulted in G1/S cell-cycle arrest. We then showed that angiotensin II type 1 receptor (AGTR1) was a direct target of miR-410, with miR-410 suppressing AGTR1 expression levels. In contrast, inhibition of miR-410 increased the expression of AGTR1. Silencing of AGTR1 inhibited cell growth and invasion, similar to miR-410 overexpression. In addition, we found that the induction of vascular endothelial growth factor and the activation of the ERK signaling pathway by angiotensin II were blocked by miR-410, similar to the angiotensin II inhibitor losartan. miR-410 overexpression inhibited angiogenesis in mice through the repression of CD31 expression. ERK pathway knockdown suppressed pancreatic cancer cell proliferation, invasion, and angiogenesis. Finally, we found that miR-410 was downregulated in pancreatic cancer tissues compared to adjacent nontumor tissues, whereas AGTR1 was upregulated in pancreatic cancer tissues. Pearson correlation analysis showed that miR-410 and AGTR1 were inversely expressed. In conclusion, our data indicate that miR-410 suppresses pancreatic cancer growth, cell invasion, migration, and angiogenesis via the downregulation of AGTR1, acting as a tumor-suppressive miRNA. In addition, our results suggest that miR-410 is a potential diagnostic biomarker and therapeutic target for patients with pancreatic cancer. V C 2015 IUBMB Life, 67(1): [42][43][44][45][46][47][48][49][50][51][52][53] 2015

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MicroRNA expression profiling of thymic epithelial tumors

Cited by 43 publications

References 38 publications

Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

MicroRNA‑216b reduces growth, migration and invasion of pancreatic ductal adenocarcinoma cells by directly targeting ρ‑associated coiled‑coil containing protein kinase 1

MicroRNA‐410 functions as a tumor suppressor by targeting angiotensin II type 1 receptor in pancreatic cancer

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