MicroRNA expression profiling of male breast cancer

Fassan, Matteo; Baffa, Raffaele; Palazzo, Juan; Lloyd, Joshua M.; Crosariol, Marco; Liu, Chang Gong; Volinia, Stefano; Alder, Hannes; Rugge, Massimo; Croce, Carlo M.; Rosenberg, Anne

doi:10.1186/bcr2348

Cited by 103 publications

(92 citation statements)

References 41 publications

(65 reference statements)

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“…The identification of tumor-specific miRNAs and their target genes seems to be crucial to validate the molecular mechanism in tumorgenesis. has been reported to be involved in some malignant tumors [16,17]. Here, we demonstrated the relation of miR-191, NDST1, and MGC803 cell proliferation.…”

Section: Ndst1 Could Suppress Mgc803 Cell Proliferationmentioning

confidence: 66%

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MicroRNA-191 targets <italic>N</italic>-deacetylase/<italic>N</italic>-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

Shi¹,

Su²,

Long³

et al. 2011

ABBS

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As a family of post-transcriptional regulator of gene expression, the microRNAs (miRNAs) control a wide array of biological processes including cell differentiation, proliferation and apoptosis, and the dysregulation of miRNAs is a hallmark of cancer. Here, we found that the was at a high-expression level in human gastric adenocarcinoma cell line MGC803 and human gastric cancer tissues. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays showed that miR-191 could promote cell growth and suppress apoptosis of MGC803 cells. The N-deacetylase/N-sulfotransferase 1 (NDST1) was confirmed to be a direct target gene of miR-191 by enhanced green fluorescent protein reporter experiment. The mRNA and protein levels of NDST1 were inversely correlated with miR-191 in MGC803 cells, suggesting the negative regulation of NDST1 by miR-191. Furthermore, NDST1 played an inhibitory role and could suppress MGC803 cell proliferation. Our findings suggested that miR-191 could act as an oncogene in MGC803 cells, and the cellular function was partially due to its negative regulation of NDST1.

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Section: Ndst1 Could Suppress Mgc803 Cell Proliferationmentioning

confidence: 66%

“…MiR-191 is found to be associated with some malignant tumors, such as ovarian cancer [16] and male breast cancer [17]. MiR-191 is also differentially expressed in T regulatory cells of Type 1 diabetic patients [18].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-191 targets <italic>N</italic>-deacetylase/<italic>N</italic>-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

Shi¹,

Su²,

Long³

et al. 2011

ABBS

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“…The need for more refined therapeutic treatments for male breast cancer (MBC) is evidenced by a steady stream of publications highlighting gender-specific differences using IHC (1-5), genetics (6)(7)(8)(9)(10)(11), and more recently, epigenetics (12)(13)(14)(15). Of note, although MBC is similar histologically to female breast cancer (FBC), with the same panel of biomarkers used to guide treatment and prognosis, more rigorous interrogation of the underlying genetics shows heterogeneity in MBC as recognized in FBC where molecular profiling has identified different subgroups that correlate with varying clinical outcomes.…”

Section: Introductionmentioning

confidence: 99%

A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer

Humphries

Rajan

Droop

et al. 2017

Clinical Cancer Research

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Purpose: Breast cancer affects both genders, but is understudied in men. Although still rare, male breast cancer (MBC) is being diagnosed more frequently. Treatments are wholly informed by clinical studies conducted in women, based on assumptions that underlying biology is similar.Experimental Design: A transcriptomic investigation of male and female breast cancer was performed, confirming transcriptomic data in silico. Biomarkers were immunohistochemically assessed in 697 MBCs (n ¼ 477, training; n ¼ 220, validation set) and quantified in pre-and posttreatment samples from an MBC patient receiving everolimus and PI3K/mTOR inhibitor.Results: Gender-specific gene expression patterns were identified. eIF transcripts were upregulated in MBC. eIF4E and eIF5 were negatively prognostic for overall survival alone (log-rank P ¼ 0.013; HR ¼ 1.77, 1.12-2.8 and P ¼ 0.035; HR ¼ 1.68, 1.03-2.74, respectively), or when coexpressed (P ¼ 0.01; HR ¼ 2.66, 1.26-5.63), confirmed in the validation set. This remained upon multivariate Cox regression analysis [eIF4E P ¼ 0.016; HR ¼ 2.38 (1.18-4.8), eIF5 P ¼ 0.022; HR ¼ 2.55 (1.14-5.7); coexpression P ¼ 0.001; HR ¼ 7.04 (2.22-22.26)]. Marked reduction in eIF4E and eIF5 expression was seen post BEZ235/everolimus, with extended survival.Conclusions: Translational initiation pathway inhibition could be of clinical utility in MBC patients overexpressing eIF4E and eIF5. With mTOR inhibitors that target this pathway now in the clinic, these biomarkers may represent new targets for therapeutic intervention, although further independent validation is required.

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“…However, while FBC has been intensively investigated by comprehensive molecular analysis, at present no gene expression data are available for male breast cancer. Recently, Fassan et al reported a set of microRNA as differentially expressed by comparison of 23 male and 10 female breast cancer samples [10].…”

Section: Introductionmentioning

confidence: 99%

Gene expression analysis reveals a different transcriptomic landscape in female and male breast cancer

Callari

Cappelletti

Cecco

et al. 2010

Breast Cancer Res Treat

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Results -Almost 1000 genes were found differentially expressed (FDR<1%) between female and male patients and biological interpretation highlighted a gender associated modulation of key biological processes ranging from energy metabolism to regulation of translation and matrix remodeling as well as immune system recruitment. Moreover, an analysis of genes correlated to steroid receptors and ERBB2 suggested a prominent role for the androgen receptor in MBC with a minor relevance for progesterone receptor and ERBB2, although, similarly to FBC, a genomic amplification could be observed.Conclusions -Our findings support the idea that breast cancer is a quite different disease in male and female patients and the underlying gender related biological differences are likely to have clinical implications connected with different susceptibility to treatment.3

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MicroRNA expression profiling of male breast cancer

Cited by 103 publications

References 41 publications

MicroRNA-191 targets <italic>N</italic>-deacetylase/<italic>N</italic>-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

MicroRNA-191 targets <italic>N</italic>-deacetylase/<italic>N</italic>-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer

Gene expression analysis reveals a different transcriptomic landscape in female and male breast cancer

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MicroRNA expression profiling of male breast cancer

Cited by 103 publications

References 41 publications

MicroRNA-191 targets &lt;italic&gt;N&lt;/italic&gt;-deacetylase/&lt;italic&gt;N&lt;/italic&gt;-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

MicroRNA-191 targets &lt;italic&gt;N&lt;/italic&gt;-deacetylase/&lt;italic&gt;N&lt;/italic&gt;-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer

Gene expression analysis reveals a different transcriptomic landscape in female and male breast cancer

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MicroRNA-191 targets <italic>N</italic>-deacetylase/<italic>N</italic>-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

MicroRNA-191 targets <italic>N</italic>-deacetylase/<italic>N</italic>-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803