MicroRNA expression profiling of dibenzalacetone (DBA) treated intracellular amastigotes of Leishmania donovani

Highlighting the interplay of microRNAs from Leishmania parasites and infected-host cells

“…The antiproliferative and apoptotic effect of transdibenzalacetone (DBA, a synthetic monoketone analogue of curcumin) on L. donovani has been previously reported (Chauhan et al, 2018), and several miRNAs including hsa-miR-151a, hsa-miR-15b and hsa-miR-30c-1 were identified as down-regulated markers in DBA treated intracellular amastigotes in comparison with untreated parasites (Singh and Chauhan, 2018). On the other hand, miR-15b targets B-cell lymphoma 2 (Bcl-2) and the caspase signalling promoting apoptosis (Guo et al, 2009).…”

“…miR-151a plays a role in the regulation of cellular respiration and ATP production by targeting cytochrome b. The downregulation of miR-151a, after DBA parasites treatment, induced mitochondrial dysfunction in Leishmania parasites (Zhou et al, 2015;Singh and Chauhan, 2018). miR-30a-3p is overexpressed in Leishmania infected cells (Singh et al, 2016), however, it is downregulated in DBA-treated parasites, suggesting that the down-regulation of this miRNA could inhibit the replication and virulence of Leishmania parasites (Singh and Chauhan, 2018).…”

“…The activity of ATG4 (autophagy-related proteins) is required for parasite viability, and it has been identified as a target of miR-30c (Williams et al, 2009(Williams et al, , 2013Singh and Chauhan, 2018). The down-regulation of ATG4 inhibits the cell viability of Leishmania parasites through the regulation of miR-30c…”

Leishmania-Host Interactions—An Epigenetic Paradigm

“…Recent reports of L. donovani hijacking the host's transcription factor, c-myc and reduction of intramacrophagic parasite burden upon c-myc silencing or inhibition, with consequent miRNA upregulation, implicate c-myc as a potential therapeutic target (36). Epigenetic targets such as miRNAs screened in L. donovani -infected macrophages upon treatment with antileishmanial trans-dibenzalacetone, revealed an imbalance between apoptosis and autophagy (86).…”

Features of geometric and electronic structure 2,4- dinitrophenylhydrazones of benzalacetone and dibenzalacetone

“…Бензальацетон (I) и дибензальацетон (II) обладают набором ценных свойств, что позволяет использовать их в медицине, при получении оптических материалов, ароматизаторов и других важных продуктов [1][2][3][4][5]. Геометрическое строение молекулы (IV) существенно отличается от строения молекулы (III) (рис.…”