MicroRNA expression profiles in trophoblastic cells

Prieto, DM Morales; Weber, Maja; Ospina‐Prieto, Stephanie; Hölters, Sebastian; Schleußner, E; Markert, Udo R.

doi:10.1055/s-0031-1293422

Cited by 25 publications

(42 citation statements)

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“…However, the signature of miRNAs in the placenta has yet to be elucidated. Recently, analyses of the expression of small RNAs in the placenta by small RNA library sequencing confirmed that most placenta-specific miRNAs were linked to the C19 MC cluster and some of them have been reported to be associated with pre-eclampsia or premature labor (14). However, the possible involvement of the C19 MC cluster in GDM remains to be elucidated.…”

Section: Expression Of Luciferase Gene With Mutated or Wild-type Pparmentioning

confidence: 99%

“…In a microarray analysis previously performed by our group, the microRNA expression profiles in placentas of patients with GDM were compared with those of normal placentas, which revealed that miR-518d was differentially expressed in placentas of patients with GDM. miR-518d has been reported to be upregulated >10-fold in third trimester trophoblast cells compared with first trimester trophoblast cells, indicating that miR-518d may be involved in the regulation of trophoblast proliferation and invasion (14). However, the involvement of placental miR-518d in the molecular mechanisms of GDM requires to be elucidated.…”

Section: Expression Of Luciferase Gene With Mutated or Wild-type Pparmentioning

confidence: 99%

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MicroRNA-518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus

Zhao

Zhang

Shi

et al. 2014

Molecular Medicine Reports

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The placenta is thought to have a critical role in the pathogenesis of gestational diabetes mellitus (GDM), as GDM‑associated complications resolve following delivery. Placenta-specific microRNAs (miRNAs) may contribute to the pathology of the development of GDM. The aim of the present study was to evaluate whether the placenta-specific miR-518d contributes to the development of GDM. It was revealed that miR-518d expression was higher in placentas taken from patients with GDM compared with control placentas, whereas the protein levels of the predicted miR-518d target gene, peroxisome proliferator-activated receptor-α (PPARα), were lower in placentas from patients with GDM compared with those from control subjects. It was also demonstrated that PPARα was a direct target of miR-518d with a specific binding site at the seed sequence, which determines target specificity. In the placentas of females with GDM increased levels of miR-518d were negatively correlated with the levels of PPARα protein. As PPARα dysregulation may be related to the development of GDM, it is suggested that upregulation of miR-518d may be associated with the pathogenesis of GDM via an effect on the regulation of PPARα expression.

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Section: Expression Of Luciferase Gene With Mutated or Wild-type Pparmentioning

confidence: 99%

Section: Expression Of Luciferase Gene With Mutated or Wild-type Pparmentioning

confidence: 99%

MicroRNA-518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus

Zhao

Zhang

Shi

et al. 2014

Molecular Medicine Reports

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“…MicroRNAs (miRNAs) belong to a family of small non‐coding RNAs made up of 20–25 nucleotides that inversely mediate gene expression after transcription . In recent years, projects have applied miRNA microarray methods to locate distinctly expressed miRNAs in the placentas of normal and PE patients . These studies have indicated that a variety of distinctly expressed miRNAs, such as miR‐29b, miR‐222 and miR‐16, potentially play important roles in PE etiology .…”

Section: Introductionmentioning

confidence: 99%

MiRNA‐320a inhibits trophoblast cell invasion by targeting estrogen‐related receptor‐gamma

Gao

Deng

Wang

2018

J of Obstet and Gynaecol

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“…Typical changes in expression of miRNAs from these three clusters during pregnancy suggest their potential involvement in physiological processes [36]. For example, expression of miRNAs from cluster C19MC increases continually from first to third trimester and closely correlates with placenta growth [37]. miRNAs are released from placenta, primarily from placental trophoblast, into maternal and fetal circulation mainly via exosomes [28].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide miRNA profiling in plasma of pregnant women with Down syndrome fetuses

Svobodová

Chylíková

Šeda

et al. 2019

Preprint

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Objective: Down syndrome (DS) belongs to the most common causes of mental retardation in men, therefore new approaches allowing its rapid and effective prenatal detection are being explored. In our study we focused on diagnostic potential of plasma microRNAs. We follow up on our previous study in DS placentas, where seven miRNAs were found as being significantly elevated.Methods: A total of 70 first-trimester plasma samples of pregnant women were included to the present study (35 samples with DS fetuses; 35 with euploid fetuses). The genome-wide miRNA profiling was performed in the preliminary study using Affymetrix GeneChip™ miRNA 4.1 Array Strips.Selected miRNAs were then included to the following validation study using qPCR. Results: Based on the current pilot study results (12 miRNAs), our previous research on CVS samples (7 miRNAs) and literature (4 miRNAs), the group of 23 miRNAs were selected for validation study. Although the results of the preliminary study were promising, the following validation step using more sensitive technology RT-PCR and larger group of samples revealed no significant differences in miRNA profiles between compared groups. Conclusion: Our results suggest that testing of the first trimester plasma miRNAs is not suitable for NIPT. Different results could be theoretically achieved at later gestational ages; however, such a result probably would have limited utilization in clinical practice.

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MicroRNA expression profiles in trophoblastic cells

Cited by 25 publications

References 0 publications

MicroRNA-518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus

MicroRNA-518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus

MiRNA‐320a inhibits trophoblast cell invasion by targeting estrogen‐related receptor‐gamma

Genome-wide miRNA profiling in plasma of pregnant women with Down syndrome fetuses

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