MicroRNA Expression Profiles in Superficial Esophageal Squamous Cell Carcinoma before Endoscopic Submucosal Dissection: A Pilot Study

Abstract

Esophageal squamous cell carcinoma (ESCC) has a poor prognosis when diagnosed at an advanced stage, and early detection and treatment are essential to improve survival. However, intraobserver and interobserver variation make the diagnosis of superficial ESCC difficult, and suitable biomarkers are urgently needed. Here, we compared the microRNA (miRNA) expression profiles of superficial ESCC tissues and adjacent normal tissues obtained immediately before esophageal endoscopic submucosal dissection. We found that ESCC and normal tissues differed in their miRNA expression profiles. In particular, miR-21-5p and miR-146b-5p were significantly upregulated and miR-210-3p was significantly downregulated in tumor tissues compared with normal tissues. We also detected significant associations between miRNA expression and ESCC invasion depth and lymphovascular invasion. The same differential expression of miR-21-5p, miR-146b-5p, and miR-210-3p was detected in ESCC cell lines compared with normal esophageal epithelial cells in vitro. However, transfection of ESCC cells with miR-210-3p and miR-21-5p mimics or inhibitors had partial effects on cell proliferation and invasion in vitro. These results indicate that miRNA expression is significantly deregulated in superficial ESCC, and suggest that the potential contribution of differentially expressed miRNAs to the malignant phenotype should be further investigated.

An integrated analysis of dysregulated SCD1 in human cancers and functional verification of miR-181a-5p/SCD1 axis in esophageal squamous cell carcinoma

“…The most recent findings on the potential benefits of mRNAs indicate they may serve as prognostic factors in ESCC [49] . For instance, miR-10, miR-18, miR-19, miR-21, miR-25, miR-31, miR-129, miR-365, miR-451, miR-1246, and miR-1322 are upregulated in tumor tissues of ESCC patients compared with normal tissues; moreover, miR-155, miR-203, miR-205, miR375, and miR-718 are downregulated in ESCC patients compared to control groups 50 , 51 . Furthermore, several investigations have determined that miRNAs could be accessible noninvasive biomarkers to monitor responses to therapy, such as radiotherapy and chemotherapy.…”

Role of Mir-452-5p Overexpression in Epithelial–Mesenchymal Transition (EMT) in Early-stage Colorectal Cancer

“…Biopsy samples of the tumor and adjacent normal tissue were taken from each patient immediately before completing ESD. The sampling procedure was the same as that described in our previous study (12). All tissue samples were frozen immediately after excision and in vivo 37: 1980-1990 (2023) 1981 1,525760 0,000372 0,010005 Down-regulated hsa-miR-133b 0,324593 0,000013 0,001033 hsa-miR-133a-3p 0,380456 0,000151 0,004900 hsa-miR-378g 0,444162 0,000005 0,001033 hsa-miR-378c 0,452104 0,000016 0,001033 hsa-miR-378d 0,454092 0,000019 0,001033 hsa-miR-378e 0,464034 0,000015 0,001033 hsa-miR-378f 0,467831 0,000009 0,001033 hsa-miR-378b 0,469599 0,000001 0,000433 hsa-miR-378i 0,470580 0,000012 0,001033 hsa-miR-572 0,479847 0,000412 0,010528 hsa-miR-378a-5p 0,516377 0,000044 0,001854 hsa-miR-3185 0,555634 0,000279 0,008322 hsa-miR-378a-3p 0,558695 0,000002 0,000677 hsa-miR-638 0,610290 0,000324 0,009267 hsa-miR-4731-5p 0,647158 0,000019 0,001033 Fold change (FC)>1.5 and FC<0.67, p<0.001.…”

“…Microarray analysis of miRNAs. A miRNA array analysis was performed as described in our previous study (12). We used the miRNAeasy Mini Kit (Qiagen, Hilden, Germany) to extract total RNA from tissue samples according to the manufacturer's instructions.…”

Novel milestones for early esophageal carcinoma: From bench to bed

“…MiRNAs can be obtained not only from body fluids but also from tissues. Fujihara et al [ 58 ] obtained matched sets of primary ESCC tissue samples and corresponding neighboring healthy tissues from patients with a pathological diagnosis of stage I ESCC. Then, unsupervised hierarchical cluster analysis was used to determine the variation in gene expression patterns between ESCC tissues and neighboring healthy tissues, and 48 differentially expressed miRNAs were revealed.…”