MicroRNA expression profiles in placenta with severe preeclampsia using a PNA-based microarray

Choi, Song‐Yi; Yun, Ji Eun; Lee, O J; Han, Huansheng; Yeo, Min‐Kyung; Lee, M A; Suh, Kwang Sun

doi:10.1016/j.placenta.2013.06.006

Cited by 140 publications

(99 citation statements)

References 37 publications

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“…25,26 The placenta is a source (and site) of action of mediators of these processes, and plays a central role in pre-eclampsia pathogenesis. 26,27 Conventional and microarray approaches have identified a substantial number of differentially expressed genes, including miRNAs, in human placentas from patients with pre-eclampsia compared with normotensive healthy term controls, 19,20,28 and miRNAs clearly possess critical roles in regulating placental development and function. 29 miR-126 is highly expressed in endothelial cells and exerts an important function in maintaining endothelial homeostasis and vascular integrity.…”

Section: Discussionmentioning

confidence: 99%

Decreased placental miR-126 expression and vascular endothelial growth factor levels in patients with pre-eclampsia

Hong

2014

J Int Med Res

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Objectives: To detect microRNA (miR)-126 expression and its correlation with vascular endothelial growth factor (VEGF) levels in placentas from patients with pre-eclampsia compared with those from normotensive pregnancies. Methods: miR-126 expression, and VEGF mRNA and protein levels, in placentas collected sequentially from patients with pre-eclampsia and normotensive pregnancies were measured using real-time reverse transcription-polymerase chain reaction and Western blot, respectively. The relationship between miR-126 and VEGF expression was analysed statistically. The regulatory effect of miR-126 on VEGF expression in human placental choriocarcinoma (BeWo) cells in vitro was also investigated. Results: miR-126 expression was decreased, and VEGF mRNA and protein levels were significantly lower, in placentas from patients with pre-eclampsia (n ¼ 115) compared with placentas from normotensive pregnancies (n ¼ 115). A positive correlation was found between VEGF mRNA and miR-126 expression (r ¼ 0.79). In addition, miR-126 overexpression significantly upregulated VEGF expression in BeWo cells, whereas miR-126 downregulation decreased VEGF expression. Conclusions: miR-126 was downregulated in placentas from patients with pre-eclampsia and this correlated with decreased VEGF expression. These findings indicate that miRNA-126 may be involved in pre-eclampsia pathogenesis and could be a potential biomarker for this disease.

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Section: Discussionmentioning

confidence: 99%

Decreased placental miR-126 expression and vascular endothelial growth factor levels in patients with pre-eclampsia

Hong

2014

J Int Med Res

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“…miR-197 was also lower in biopsy-proven nonalcoholic fatty liver (Celikbilek et al, 2014), primary biliary cirrhosis (Ninomiya et al, 2013), multiple sclerosis1 (Margareta Jernås et al, 2013), uterine leiomyoma (Ling et al, 2015a), type 2 diabetes (Zampetaki et al, 2010) and increased in placentas of preeclampsia (Choi et al, 2013), Graves' disease (Qin et al, 2015). Furthermore, miR-197, in hepatitis B virus infected patients, gradually decreased along with the severity of liver disease symptoms.…”

Section: Mir-197 In Othersmentioning

confidence: 98%

miR-197: A novel biomarker for cancers

Wang

Chen

et al. 2016

Gene

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“…However, no deregulation of miR-210 in PE versus control placentas was observed. Two additional studies performed using formalin-fixed paraffin-embedded placenta tissues also failed to demonstrate altered expression of mir-210 in PE cases [52,53]. Choi et al examined 21 formalin-fixed paraffin-embedded placentas from severe PE pregnancies and 20 from uncomplicated ones by the PNA-based microarray method and identified 13 miRNAs upregulated and 2 downregulated in PE placentas compared to controls with miR-92b showing the higher change in PE patients [52].…”

Section: Notch1 Jagged1mentioning

confidence: 99%

miRNAs in pregnancy-related complications

Lycoudi

Mavreli

Mavrou

et al. 2015

Expert Review of Molecular Diagnostics

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MicroRNAs (miRNAs) constitute a highly conserved class of small non-coding RNAs, involved in post-transcriptional regulation processes by modifying the expression of specific mRNAs. During placental development, cell differentiation, adhesion, migration, apoptosis and angiogenesis are regulated by specific miRNAs and aberrant expression has been associated with the pathogenesis of pregnancy-related complications. Recent studies focusing on placental and maternal peripheral blood miRNA profiling showed different expression between normal and complicated pregnancies, providing valuable information about the pathophysiological role of miRNAs and identifying potential biomarkers for monitoring pregnancy complications. This review summarizes the current knowledge in the field and presents the possible use of miRNAs as biomarkers for early detection and monitoring of these complications.

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MicroRNA expression profiles in placenta with severe preeclampsia using a PNA-based microarray

Cited by 140 publications

References 37 publications

Decreased placental miR-126 expression and vascular endothelial growth factor levels in patients with pre-eclampsia

Decreased placental miR-126 expression and vascular endothelial growth factor levels in patients with pre-eclampsia

miR-197: A novel biomarker for cancers

miRNAs in pregnancy-related complications

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