MicroRNA expression profiles in human CD3+ T cells following stimulation with anti-human CD3 antibodies

Sousa, Isabel Garcia; Almo, Manuela Maragno do; Simi, Kelly Cristina Rodrigues; Bezerra, Maryani Andressa Gomes; Andrade, Rosângela Vieira de; Maranhão, Andréa Queiroz; Brígido, Marcelo de Macedo

doi:10.1186/s13104-017-2442-y

Cited by 9 publications

(10 citation statements)

References 50 publications

(53 reference statements)

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“…CD3 + can reflect the level of all T lymphocytes in the body, and CD4 + can positively modulate the immunity (6). CD8 + is able to kill and destroy cells, which can inhibit CD4 + and aggravate the immune dysfunction of patients (17). It was uncovered in the present study that the observation group had elevated CD3 + , CD4 + and CD4 + /CD8 + levels and lower CD8 + level in comparison with the control group.…”

Section: Discussionsupporting

confidence: 46%

Efficacy and prognosis of surgical treatment in ARMM and its effects on immune and inflammatory profiles

2020

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The aim of the present study was to investigate the efficacy and prognosis of surgical treatment in anorectal malignant melanoma (ARMM) as well as its effects on immune and inflammatory profiles. A total of 64 patients with ARMM were enrolled and divided into control group (n=32) and observation group (n=32) based on different therapeutic methods. Patients in the observation group underwent wide local excision, while those in the control group were given non-surgical treatment. The immune function was compared between the two groups after treatment. The expression levels of serum IL-17, IL-23 and vascular endothelial growth factor (VEGF) were compared between the groups before and after treatment. Patients were followed up for 5 years to observe the recurrence and survival rates in the two groups. Compared with those in the control group, the levels of cluster of differentiation (CD)3 + , CD4 + and CD4 + /CD8 + were increased, while the level of CD8 + was reduced in the observation group (P<0.05). The levels of serum IL-17, IL-23 and VEGF were significantly lower in both groups at 4, 6 and 8 weeks after treatment than those before treatment, and the decrease was more obvious in the observation group (P<0.05). The observation group exhibited a notably elevated 5-year survival rate and a remarkably decreased recurrence rate in comparison with the control group (P<0.05). For ARMM, surgical treatment is more effective than non-surgical treatment, which has less effect on immune function, is able to reduce serum IL-17, IL-23 and VEGF levels, lower recurrence rate, improve patient survival rate, and plays a certain positive role in prolonging the survival time of ARMM patients.

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Section: Discussionsupporting

confidence: 46%

Efficacy and prognosis of surgical treatment in ARMM and its effects on immune and inflammatory profiles

2020

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“…The up-regulated miR-101 and miR-381 are involved in the polarization and activation of the cells of the innate immune compartment, regulating the inflammatory response ( Zhu et al, 2010 ; Essandoh et al, 2016 ; Wen et al, 2016 ); the increased miR-548 may represent a mechanism facilitating viral pathogenesis as it negatively correlates with IFNγR1 levels ( Xing et al, 2014 ). miR-21 and miR-590, up-regulated by HHV-6A, are involved in differentiation and activation of T cells, particularly during autoimmunity ( Sousa et al, 2017 ), with miR-590 also down-regulating critical genes of signaling pathways similar in cancer and inflammation ( Sheikholeslami et al, 2017 ). miR-29 and miR-15, both increased by HHV-6A infection, have a specific role in regulating the cytotoxic activity of NK cells, inhibiting the production of IFNγ by directly targeting the 3′ UTR of its mRNA ( Ma et al, 2011 ; Leong et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

HHV-6A/6B Infection of NK Cells Modulates the Expression of miRNAs and Transcription Factors Potentially Associated to Impaired NK Activity

et al. 2017

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Natural killer (NK) cells have a critical role in controlling virus infections, and viruses have evolved several mechanisms to escape NK cell functions. In particular, Human herpesvirus 6 (HHV-6) is associated with diseases characterized by immune dysregulation and has been reported to infect NK cells. We recently found that HHV-6 in vitro infection of human thyroid follicular epithelial cells and T-lymphocytes modulates several miRNAs associated with alterations in immune response. Since miRNAs are key regulators of many immune pathways, including NK cell functions, we aimed to study the impact of HHV-6A and -6B in vitro infection on the intracellular mediators correlated to NK cell function. To this purpose, a human NK cell line (NK-92) was infected in vitro with HHV-6A or 6B and analyzed for alterations in the expression of miRNAs and transcription factors. The results showed that both viruses establish lytic replication in NK-92 cells, as shown by the presence of viral DNA, expression of lytic transcripts and antigens, and by the induction of an evident cytopathic effect. Notably, both viruses, although with species-specific differences, induced significant modifications in miRNA expression of miRNAs known for their role in NK cell development, maturation and effector functions (miR-146, miR-155, miR-181, miR-223), and on at least 13 miRNAs with recognized role in inflammation and autoimmunity. Also the expression of transcription factors was significantly modified by HHV-6A/6B infection, with an early increase of ATF3, JUN and FOXA2 by both species, whereas HHV-6A specifically induced a 15-fold decrease of POU2AF1, and HHV-6B an increase of FOXO1 and a decrease of ESR1. Overall, our data show that HHV-6A and -6B infections have a remarkable effect on the expression of miRNAs and transcription factors, which might be important in the induction of NK cell function impairment, virus escape strategies and related pathologies.

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“…Cells were stimulated during different lengths of time ranging from 18 h (18 h) to 7 days (7 d). The studies included in the table are: A ( 5 ), B ( 6 ), C ( 7 ), D ( 8 ), E ( 9 ), F ( 10 ), G ( 11 ). Whenever more than one detection method was used, only consistent data obtained with at least two techniques was selected ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Control of Immunoregulatory Molecules by miRNAs in T Cell Activation

2018

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MiRNA targeting of key immunoregulatory molecules fine-tunes the immune response. This mechanism boosts or dampens immune functions to preserve homeostasis while supporting the full development of effector functions. MiRNA expression changes during T cell activation, highlighting that their function is constrained by a specific spatiotemporal frame related to the signals that induce T cell-based effector functions. Here, we update the state of the art regarding the miRNAs that are differentially expressed during T cell stimulation. We also revisit the existing data on miRNA function in T cell activation, with a special focus on the modulation of the most relevant immunoregulatory molecules.

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MicroRNA expression profiles in human CD3+ T cells following stimulation with anti-human CD3 antibodies

Cited by 9 publications

References 50 publications

Efficacy and prognosis of surgical treatment in ARMM and its effects on immune and inflammatory profiles

Efficacy and prognosis of surgical treatment in ARMM and its effects on immune and inflammatory profiles

HHV-6A/6B Infection of NK Cells Modulates the Expression of miRNAs and Transcription Factors Potentially Associated to Impaired NK Activity

Control of Immunoregulatory Molecules by miRNAs in T Cell Activation

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