microRNA Expression Profile in Patients with Stage II Colorectal Cancer: A Turkish Referral Center Study

Tanoğlu, Alpaslan; Balta, Ahmet Ziya; Berber, Ufuk; Özdemi̇r, Yavuz; Emirzeoglu, Levent; Sayılır, Abdurrahim; Sücüllü, İlker

doi:10.7314/apjcp.2015.16.5.1851

Cited by 20 publications

(13 citation statements)

References 30 publications

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“…Expression of lncRNAs in tumors is significantly regulated by epigenetic and genomic modifications; hence, these lncRNAs can control both protein-coding and non-coding genes and interact with known other cancer genes [21,22]. Long non-coding RNAs have different features that act like tumor suppressors, oncogenes, or in their therapeutic potentials [23][24][25]. Their complicated structures and ability to be involved in multicomponent complexes are thought to be the main cause of these differences.…”

Section: Tumor Suppressor and Oncogenic Long Non-coding Rnasmentioning

confidence: 99%

Tumor Suppressor and Oncogenic Role of Long Non-Coding RNAs in Cancer

Güzel

Okyay²,

Yalcinkaya

et al. 2019

North Clin Istanbul

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Non-coding RNAs are RNA molecules that are not translated into the protein, making up the vast majority of the human genome. Long non-coding RNAs (lncRNA) are in the RNA group that has longer than 200 nucleotides, and non-protein coding transcripts. In recent years, the potential has attracted considerable attention as new important biological regulators. LncRNAs play a critical role in regulating the activity and localization of proteins, processing the production of small RNAs, and processing other RNAs. They are also involved in cell differentiation, cell cycle, proliferation, apoptosis, migration and invasion by modulation of gene expression. Abnormal expression of LncRNAs has an important role in the function of oncogenes and tumor suppressor genes. Recently, there has been an increasing number of studies on the tumorigenic effects of specific lncRNAs in the initiation and progression of cancer. In this review, general information about lncRNAs is provided, including the biological importance of lncRNAs in cancer diseases and their potential development in therapeutic applications.

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Section: Tumor Suppressor and Oncogenic Long Non-coding Rnasmentioning

confidence: 99%

Tumor Suppressor and Oncogenic Role of Long Non-Coding RNAs in Cancer

Güzel

Okyay²,

Yalcinkaya

et al. 2019

North Clin Istanbul

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“…A significant deregulation of this miRNA has been observed in CRC patients after chemotherapy [35], besides a negative fold change of miRNA-145 in stages II, III and IV CRC [36]. Tanaglu et al [37] analyzed 16 miRNA expression profiles of 40 patients with recurrent and non-recurrent CRC, finding that the miRNA-145 expression was down-regulated. These data suggest miRNA-145 as a good biomarker for an early detection of this disease [20].…”

Section: Mirna Function Reference Mirna-129mentioning

confidence: 99%

“…Polymerase (cloned) (Invitrogen) catalyzed the addition of adenosine to the 3' end of RNA in a sequence-independent fashion. The parameters used were as follows: 37 Table 2: Primers designed for the amplification by qPCR.…”

Section: Rna Isolation and Real Time-pcrmentioning

confidence: 99%

Novel miRNA Sponges to Specifically Modulate Gene Expression in Colon Cancer Cells

Aguilera¹,

Perazzoli²,

Cabeza³

et al. 2019

Preprint

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miRNA sponges allow the selective blockade of a complete family of associated miRNAs which induce posttranscriptional gene silencing in its target through binding to 3´UTR mRNA. MiRNA-365 and miRNA-145 are down-regulated in colorectal cancer (CRC), but not in health tissues. Based on this, we constructed two vectors by inserting miRNA sponge (one for miRNA-365 and other for miRNA-145), and used EGFP (enhanced green fluorescent protein) as a 3′ UTR reporter gene to analyse the ability of each sponge to catch its respective miRNA. qPCR results corroborated that the expression levels of both miRNAs were lower in CRC cell lines than in normal colon cell line. Flow cytometry analysis revealed a decrease of the EGFP expression levels in the cell lines transfected with both sponges, being higher on the normal cell line while CRC cell lines presented a minimal decline. Also, this decrease was inversely proportional to the levels of expression of both miRNAs obtained by qPCR. These results were corroborated by fluorescence microscopy, showing a similar decrease fluorescence. We propose a new vector system to carry in a specific way the expression of genes to CRC cells without affecting healthy cells, preventing damage to healthy tissues.

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“…Similarity, qPCR evaluating microdissected preoperative biopsies from patients with rectal cancer showed a significant correlation between miR-133b and distant-metastasis-free survival [ 82 ]. In all resected colon cancer tissue without patient's recurrence, the miR-133b expression was significantly upregulated [ 83 ]. High expression of miR-185 and low expression of miR-133b were correlated with poor survival and metastasis in colorectal cancer [ 84 ], which suggest the potential prognostic values of these miRNAs for predicting clinical outcome after surgery, miR-1 and mir-133b have been significantly downregulated in recurrent PCa specimens and can serve as novel biomarkers for prediction of PCa progression [ 85 ].…”

Section: Introductionmentioning

confidence: 99%

miR-133b, a particular member of myomiRs, coming into playing its unique pathological role in human cancer

Xia

Chen

et al. 2017

Oncotarget

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MicroRNAs, a family of single-stranded and non-coding RNAs, play a crucial role in regulating gene expression at posttranscriptional level, by which it can mediate various types of physiological and pathological process in normal developmental progress and human disease, including cancer. The microRNA-133b originally defined as canonical muscle-specific microRNAs considering their function to the development and health of mammalian skeletal and cardiac muscles, but new findings coming from our group and others revealed that miR-133b have frequently abnormal expression in various kinds of human cancer and its complex complicated regulatory networks affects the tumorigenicity and development of malignant tumors. Very few existing reviews on miR-133b, until now, are principally about its role in homologous cluster (miR-1, −133 and -206s), however, most of constantly emerging new researches now are focused mainly on one of them, so In this article, to highlight the unique pathological role of miR-133b playing in tumor, we conduct a review to summarize the current understanding about one of the muscle-specific microRNAs, namely miR-133b, acting in human cancer. The review focused on the following four aspects: the overview of miR-133b, the target genes of miR-133b involved in human cancer, the expression of miR-133b and regulatory mechanisms leading to abnormal expression of miR-133b.

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microRNA Expression Profile in Patients with Stage II Colorectal Cancer: A Turkish Referral Center Study

Cited by 20 publications

References 30 publications

Tumor Suppressor and Oncogenic Role of Long Non-Coding RNAs in Cancer

Tumor Suppressor and Oncogenic Role of Long Non-Coding RNAs in Cancer

Novel miRNA Sponges to Specifically Modulate Gene Expression in Colon Cancer Cells

miR-133b, a particular member of myomiRs, coming into playing its unique pathological role in human cancer

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