MicroRNA expression profile in non‐cancerous colonic tissue associated with lymph node metastasis of colon cancer

Abstract

OBJECTIVE:To identify microRNA expression patterns associated with the lymph node metastasis of colon cancer. METHODS:MicroRNA were isolated from six frozen non-cancerous surrounding colonic tissues derived from stage II-III colon cancer patients with (n = 3) and without (n = 3) lymph node metastasis. We compared the microRNA expression profiles of the six non-cancerous colonic tissues from two colon cancer patient groups; those with confirmed lymph node metastasis, termed the lymph node positive group, and those without detectable lymph node metastasis, termed the lymph node negative group. MicroRNA expression was analyzed with Agilent microarrays containing 723 human microRNA probes. We validated the expression level of differentially expressed microRNA using quantitative real-time PCR analysis. RESULTS:Two microRNA (hsa-miR-129*, hsa-miR-137) were differentially expressed in the lymph node positive group compared with the lymph node negative group. The expression level of hsa-miR-137 was quantified via quantitative real-time PCR analysis for validation. Hsa-miR-137 expression was significantly upregulated nearly 6.6-fold in lymph node positive specimens (P = 0.036). The quantitative real-time PCR result correlates with the microarray finding. CONCLUSION:The non-cancerous colonic tissues from colon cancer patients with lymph node metastasis have a significantly different microRNA expression profile compared to that from colon cancer patients without lymph node metastasis. The differentially expressed microRNA could have relevance to the lymph node metastasis of colon cancer and may provide a simple profiling method to assist in identifying patients with lymph node metastasis. Besides, these data might offer new ideas for preventing and controlling lymphatic metastasis in colon cancer.KEY WORDS: adjacent non-cancerous colonic tissue, colon cancer, hsa-miR-137, lymph node metastasis, microRNA expression profile.

Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis

“…Epigenetic regulation of miR-129-2 was reported in glioma and lung cancer (8,9). In contrast, however, miR-129 is also upregulated in several solid tumors and non-cancerous tissues from cancer patients with lymph node metastases (29,30). Our results revealed the tumor-suppressor functions of miR-129-2.miRNAs degrade or repress translation of target mRNAs.…”

microRNA expression profiles in human colorectal cancers with brain metastases

“…Given the unique feature of their targeting, each single miRNA may have over a hundred targets, and, thus, a large number of protein-coding genes may be regulated by miRNAs (21). Huang et al (22) performed a comparison of the miRNA profile of tumor-adjacent non-cancerous colonic samples in CRC with lymph node metastasis with those without lymph node metastasis. Their results indicated that hsa-miR-129* and hsa-miR-137 were significantly differentially expressed.…”

MicroRNA-129-1 acts as tumour suppressor and induces cell cycle arrest of GBM cancer cells through targeting IGF2BP3 and MAPK1

“…Interestingly, chromosome 7q32 is a frequently deleted region in many cancers 20 21. MiR-129 family seem to behave as a tumour suppressor and their decreased expression has been shown in different tumours such as: gastric,22 bladder,23 and endometrial cancer,24 hepatocellular25 and colorectal carcinoma,26 lung adenocarcinoma27 and GBM 11. However, the precise role of miR-129-1 in GBM cancer pathology remains to be defined.…”