MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen

Chen, Sheng; Liao, Liqin; Zhao, Qiqi; Zhang, Xinheng; Li, Hongxin; Lin, Wencheng; Chen, Feng; Xie, Qingmei

doi:10.1016/j.virusres.2020.198083

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…The earliest researchers believed that chicken seminal fluid lacks CD9 and CD44 bearing extracellular vesicles (Alvarez‐Rodriguez et al., 2020). The current accumulated studies indicate the presence of EVs with a classic bilayer membrane structure; particle size distributed 30–250nm and multiple marker proteins were identified (Chen et al., 2020); and the results were consistent with this study. Interestingly, the particle size range of EVs was consistent, but its peak distribution varied across studies, which may be caused by the different chicken breeds and exosome extraction methods used in the studies.…”

Section: Discussionsupporting

confidence: 89%

Characterization and proteomics of chicken seminal plasma extracellular vesicles

Luo

Guo

Huang

et al. 2021

Reprod Domestic Animals

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In mammals, seminal plasma extracellular vesicles (SPEVs) can regulate sperm motility and capacitation. The characteristics and functions of SPEVs in avians have been rarely reported. In this study, chicken SPEVs were isolated and characterized by transmission and scanning electron microscopy (TEM/SEM) and nanoparticle tracking analysis (NTA); furthermore, seven extracellular vesicle (EVs) marker proteins were detected by Western blot (WB). TEM revealed that chicken SPEVs had a classic bilayer membrane structure. NTA confirmed that the size of SPEVs was 30-250 nm, and concentration ranged from 8.0 E + 11-8.5 E + 11 particles/ml. There were 3073 SPEVs proteins identified by deep sequencing, including 2794 intracellular proteins and 279 extracellular proteins. The overlap rate of proteomes between chicken SPEVs and vesicles reported in the Vesiclepedia database reached 86%, and 360 new proteins that had not been reported by the ExoCarta and Vesiclepedia databases were identified in chicken SPEV proteomes. Gene Ontology (GO) analysis revealed that chicken SPEV proteins were mainly enriched in supplying energy and transporting protein.There were 4 IFT family proteins speculated to play an important role in sperm composition and function. Our data were compared with two previously published studies on the proteomics of chicken seminal plasma (SP) and hen uterine fluid, and some overlapping proteins described in chicken SPEVs had been identified in hen uterine fluid (545) and chicken SP (284). In conclusion, these findings will increase our understanding of the content and composition of proteome in SPEVs and provide new insights into the important role of the SPEV regulation in sperm functions.

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Section: Discussionsupporting

confidence: 89%

Characterization and proteomics of chicken seminal plasma extracellular vesicles

Luo

Guo

Huang

et al. 2021

Reprod Domestic Animals

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“…Previous studies have shown that dissociative miRNAs in semen participated in reproduction regulation ( Chen et al, 2016 ; Yang et al, 2023 ). SPEVs-coupled miRNAs are more advantageous because their double-layer membrane structure protects the cargos against enzymatic degradation or phagocytosis/immune rejection ( Chen et al, 2020 ). The 563 common miRNAs identified in this study were also enriched in metabolic pathways such as glycolysis and oxidative phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicle-coupled miRNA profiles of chicken seminal plasma and their potential interaction with recipient cells

Han,

Li,

Zong

et al. 2023

Poultry Science

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“…Nine EV-related miRNAs were found to be differentially expressed: 3 upregulated and 6 downregulated. Among these miRNAs, the tumor suppressor miR-138-5p was found to be downregulated as in many types of human cancer [ 66 ].…”

Section: Evs In Physiology and Pathophysiologymentioning

confidence: 99%

Extracellular Vesicles in Veterinary Medicine

Moccia

Sammarco

Cavicchioli

et al. 2022

Animals

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Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles involved in many physiological and pathological processes not only in humans but also in all the organisms of the eukaryotic and prokaryotic kingdoms. EV shedding constitutes a fundamental universal mechanism of intra-kingdom and inter-kingdom intercellular communication. A tremendous increase of interest in EVs has therefore grown in the last decades, mainly in humans, but progressively also in animals, parasites, and bacteria. With the present review, we aim to summarize the current status of the EV research on domestic and wild animals, analyzing the content of scientific literature, including approximately 220 papers published between 1984 and 2021. Critical aspects evidenced through the veterinarian EV literature are discussed. Then, specific subsections describe details regarding EVs in physiology and pathophysiology, as biomarkers, and in therapy and vaccines. Further, the wide area of research related to animal milk-derived EVs is also presented in brief. The numerous studies on EVs related to parasites and parasitic diseases are excluded, deserving further specific attention. The literature shows that EVs are becoming increasingly addressed in veterinary studies and standardization in protocols and procedures is mandatory, as in human research, to maximize the knowledge and the possibility to exploit these naturally produced nanoparticles.

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MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen

Cited by 7 publications

References 38 publications

Characterization and proteomics of chicken seminal plasma extracellular vesicles

Characterization and proteomics of chicken seminal plasma extracellular vesicles

Extracellular vesicle-coupled miRNA profiles of chicken seminal plasma and their potential interaction with recipient cells

Extracellular Vesicles in Veterinary Medicine

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