MicroRNA expression profile and TNM staging system predict survival in patients with lung adenocarcinoma

Xin, Guohong; Cao, Xiaoci; Zhao, Wujie; Lv, Pintian; Qiu, Gang; Li, Yaxing; Wang, Bin; Fang, Baoshuan; Jia, Yitao

doi:10.3934/mbe.2020409

Cited by 6 publications

(11 citation statements)

References 32 publications

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“…Examination of miRNAs for specific fractions can significantly increase both the sensitivity and specificity of selected circulating miRNAs as noninvasive biomarkers [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ].…”

Section: Circulating Mirnamentioning

confidence: 99%

miRNAs in Cancer (Review of Literature)

Smolarz

Durczyński

Romanowicz

et al. 2022

IJMS

117

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MicroRNAs (miRNAs) are short, noncoding, single-stranded RNA molecules that regulate gene expression at the post-transcriptional level by binding to mRNAs. miRNAs affect the course of processes of fundamental importance for the proper functioning of the organism. These processes include cell division, proliferation, differentiation, cell apoptosis and the formation of blood vessels. Altered expression of individual miRNAs has been shown in numerous cancers, which may indicate the oncogenic or suppressor potential of the molecules in question. This paper discusses the current knowledge about the possibility of using miRNA as a diagnostic marker and a potential target in modern anticancer therapies.

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Section: Circulating Mirnamentioning

confidence: 99%

miRNAs in Cancer (Review of Literature)

Smolarz

Durczyński

Romanowicz

et al. 2022

IJMS

117

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“…This finding is in line with the study of Xin et al, which constructed a prognostic model with nine miRNAs including hsa-mir-3940 by bioinformatics analysis method. 16 So, miR-3940-3p was a potential indicator for the survival of LUAD patients in an independent manner.…”

Section: Discussionmentioning

confidence: 97%

“… 14 Similarly, lncRNA DRAIC (lnc DRAIC) and miR-3940-3p have been reported as survival-related lncRNA or miRNA in patients with LUAD from TCGA database. 15 , 16 However, the detailed clinical significance and effects of lnc DRAIC and miR-3940-3p on LUAD have not been investigated and underscored.…”

Section: Introductionmentioning

confidence: 99%

Prognostic Value of lncRNA DRAIC and miR-3940-3p in Lung Adenocarcinoma and Their Effect on Lung Adenocarcinoma Cell Progression

Liu

Yang

Zhou

et al. 2021

CMAR

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“…ncRNAs with different expression levels in tumors and corresponding normal tissues have been previously selected for further clinical studies [32]. However, we performed our study from another angle.…”

Section: Ivyspringmentioning

confidence: 99%

circ_0000567/miR-421/TMEM100 Axis Promotes the Migration and Invasion of Lung Adenocarcinoma and Is Associated with Prognosis

Yang¹,

Si²,

Xiao³

et al. 2022

J. Cancer

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Purpose: Due to the high metastatic ability and poor prognosis of lung adenocarcinoma (LUAD), we identified novel non-coding RNAs, which constitute approximately 60% of human transcripts, as prognostic biomarkers and potential therapeutic targets for LUAD. Methods: In this study, we downloaded and analyzed microRNA (miRNA) datasets from The Cancer Genome Atlas (TCGA) to identify dysregulated miRNAs correlating with the overall survival (OS) of LUAD patients. miR-421, circ_0000567, and TMEM1000 expression levels were examined by quantitative real-time polymerase chain reaction (qRT-PCR) in NSCLC tissues from 73 patients and adjacent normal tissues. Cell migration and invasion were assayed using wound healing and transwell assays. miR-421 target predictions were conducted using starBase, CircInteractome, circBank, TargetScan, miRanda, MirDB, miRpath, and Gene Expression Omnibus (GEO) databases. The circular structure and stability of circ_0000567 were verified by RNase R digestion and qRT-PCR using oligo(dT) and random primers. A luciferase reporter assay was used to evaluate the relationship between miR-421, circ_0000567, and TMEM100. Results: The miRNA panel associated with OS in patients with LUAD was screened according to the hazard ratio (HR) of miRNAs from high to low. Based on the correlation between these miRNAs and OS, as well as miRNA expression levels, miR-421 was selected for further outcome analysis. High miR-421 expression was an independent risk factor for shorter OS in 73 patients collected from our department. Bioinformatic analyses, luciferase reporter assays, and functional assays showed that circ_0000567 could act as a sponge for miR-421 and prevent it from directly targeting the 3'-untranslated region of TMEM100 mRNA and further degrading it in LUAD. miR-421 expression was significantly upregulated, while circ_0000567 and TMEM100 were downregulated in tumor tissues of LUAD, compared to their counterparts in normal tissues. Gain-and loss-of-function assays showed that miR-421 promoted LUAD cell migration and invasion. Overexpression of circ_0000567 inhibited migration and invasion, whereas co-transfection of circ_0000567 and miR-421 mimics partly counteracted this effect. TMEM1000 was upregulated by enhanced circ_0000567 in LUAD cells, and the expression of TMEM1000 was inversely proportional to miR-421, whereas it was directly proportional to circ_0000567 in 73 LUAD specimens, which confirmed the competitive endogenous RNA (ceRNA) network. Conclusion: Our findings suggest that miR-421 promotes the migration and invasion of lung adenocarcinoma via circ_0000567/miR-421/TMEM100 signaling and could be a prognostic biomarker for LUAD.

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MicroRNA expression profile and TNM staging system predict survival in patients with lung adenocarcinoma

Cited by 6 publications

References 32 publications

miRNAs in Cancer (Review of Literature)

miRNAs in Cancer (Review of Literature)

Prognostic Value of lncRNA DRAIC and miR-3940-3p in Lung Adenocarcinoma and Their Effect on Lung Adenocarcinoma Cell Progression

circ_0000567/miR-421/TMEM100 Axis Promotes the Migration and Invasion of Lung Adenocarcinoma and Is Associated with Prognosis

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