MicroRNA expression patterns in indeterminate inflammatory bowel disease

Biomarkers of Inflammatory Bowel Disease

“…In the same year (2013), another paper showed that four miRNAs (miR-18a*, miR-629*, let-7b and miR-140-3p) were higher and three miRNAs were lower (miR-422a, miR-885-5p and miR-328) in the mucosa of active CD patients compared to quiescent CD patients, and two miRNAs were higher (miR-650 and miR-548a-3p) and three miRNAs were lower (miR-630, miR-489 and miR-196b) in the mucosa of active UC patients compared to quiescent UC patients (94). Another study of 2013 focused on five of the miRNAs that Wu et al (90) had identified as being differentially expressed in colonic mucosal tissue of CD active patients compared with healthy controls or UC patients (miR-19b, miR-629, miR-23b, miR-106a and miR-191), and further examined their ability to distinguish classically diagnosed indeterminate IBD (95). The expression levels of miR-19b, miR-106a and miR-629 were found to differ significantly between the UC and CD groups; all five miRNAs differed significantly between the indeterminate colitis and CD groups; and no significant difference was observed between the indeterminate and UC groups (suggesting that most cases of indeterminate colitis are likely to represent UC) (95).…”

“…Another study of 2013 focused on five of the miRNAs that Wu et al (90) had identified as being differentially expressed in colonic mucosal tissue of CD active patients compared with healthy controls or UC patients (miR-19b, miR-629, miR-23b, miR-106a and miR-191), and further examined their ability to distinguish classically diagnosed indeterminate IBD (95). The expression levels of miR-19b, miR-106a and miR-629 were found to differ significantly between the UC and CD groups; all five miRNAs differed significantly between the indeterminate colitis and CD groups; and no significant difference was observed between the indeterminate and UC groups (suggesting that most cases of indeterminate colitis are likely to represent UC) (95). Together, these studies illustrate that miRNAs have potential value as biomarkers and could possibly be developed into miRNA profile-based diagnostic tools.…”

Novel specific microRNA biomarkers in idiopathic inflammatory bowel disease unrelated to disease activity

“…[5][6][7] To date, few studies have reported aberrant miRNA expression in inflammatory bowel disease and most have focused on differentiating between ulcerative colitis and Crohn disease. [8][9][10][11][12][13] The purpose of this study was to identify novel specific miRNA markers of inflammatory bowel disease in comparison with other common forms of enterocolitis within the differential, including infectious colitis, ischemic colitis, and diverticular disease. Small RNA methodology was used not only on fresh-frozen samples but also on routinely available formalin-fixed, paraffin-embedded tissues.…”

Over expressed miRNA-31 is the Most Consistent and Unique Micro RNA in Ulcerative Colitis

“…Based on its involvement in a variety of essential cellular processes including development, cell differentiation, proliferation, and apoptosis, miRNAs are poised to make significant contributions to not only tumorigenesis, but also pathogenesis of inflammation by functioning as proinflammatory (Kurowska-Stolarska et al, 2011) or anti-inflammatory factors (Sun et al, 2013). Thus, miRNAs are implied potential as biomarkers and intervention targets against inflammatory disorders, including UC (Lin et al, 2013, Schaefer et al, 2015. Beyond these, an important feature of miRNA is their remarkable stability, for example, they can be well preserved in tissue samples even after formalin-fixation and paraffin embedding for years, and can be efficiently extracted from such specimens and variety of cell lines (Finkel et al, 2007).…”