MicroRNA Expression Characterizes Oligometastasis(es)

Lussier, Yves A.; Xing, H. Rosie; Salama, Joseph K.; Khodarev, Nikolai N.; Huang, Yong; Zhang, Qingbei; Khan, Sajid; Yang, Xinan; Hasselle, Michael D.; Darga, Thomas E.; Malik, Renuka; Fan, Huiying; Perakis, Samantha; Filippo, Matthew; Corbin, Kimberly S.; Lee, Younghee; Posner, Mitchell C.; Chmura, Steven J.; Hellman, Samuel; Weichselbaum, Ralph R.

doi:10.1371/journal.pone.0028650

Cited by 253 publications

(176 citation statements)

References 55 publications

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“…In fact, for some cancers, ectopic miR-200 expression reduces metastases (25). However, our results proposing prometastatic properties of miR-200s in breast cancer cells were later confirmed (7)(8)(9) and are further bolstered by our findings here. Moreover, the correlation between miR-200 expression and human breast cancer metastasis and relapse (7,17) and our results using human breast cancer cell lines in Figures 13-15 suggest that our results in the mouse model are not an aberration.…”

Section: Mir-200s Are Transferred In Evs From Human Metastatic Breastsupporting

confidence: 77%

“…A biphasic role of miR-200 is also supported by other studies that show that primary cancer cells downregulate miR-200 expression at the invasive front where they undergo EMT and upregulate miR-200s in the resulting metastasis where MET facilitates colonization of a distant tissue (9,26,27). Given the observed transfer of miR-200s from aggressive to less-aggressive cancer cells, it is possible that cancer cells within the primary tumor may initially release miR-200s in EVs to activate an EMT program for invasion and dissemination and then reacquire these EVs or reexpress miR-200 at the metastatic site to undergo MET for colonization.…”

Section: Mir-200s Are Transferred In Evs From Human Metastatic Breastmentioning

confidence: 52%

“…In fact, ectopic expression of the miR-200c/miR-141 cluster in 4TO7 cells enables them to colonize the lungs (6,7). Overexpression of miR-200 also promotes the colonization of certain human breast cancer cell-line xenografts (8,9).…”

Section: Introductionmentioning

confidence: 99%

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miR-200–containing extracellular vesicles promote breast cancer cell metastasis

et al. 2014

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.were taken every week. After 3 or 4 weeks, when the primary tumors approached 20 mm, the lungs were excised and lung luminescence was compared.All luminescent and fluorescent images were acquired and analyzed using Living Image software (Caliper Life Sciences).Statistics. Student's t tests, computed using Microsoft Excel, were used to analyze the significance between the treated samples and the controls where the test type was set to 1-tail distribution and 2-sample equal variance.

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Section: Mir-200s Are Transferred In Evs From Human Metastatic Breastsupporting

confidence: 77%

Section: Mir-200s Are Transferred In Evs From Human Metastatic Breastmentioning

confidence: 52%

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miR-200–containing extracellular vesicles promote breast cancer cell metastasis

et al. 2014

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“…The recent landmark CHAARTED study has shown that men presenting with oligo-metastatic prostate cancer (≤3 skeletal lesions) have improved overall survival to those with poly-metastatic disease (>3 skeletal deposits) 28 , and thus oligo-metastatic disease might represent a transitory disease phenotype with its own distinct molecular signature 29 .…”

Section: Supporting Datamentioning

confidence: 99%

Testing Radical prostatectomy in men with oligoMetastatic prostate cancer that has spread to the bone

Sooriakumaran¹

2016

http://isrctn.com/

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“…RNU-48, a small, non-coding RNA molecule, was chosen as the endogenous control. RNU-48 is expressed widely in human tissues and is stable; and is widely used as an endogenous control for microRNA expression profiling [74]. Further, this molecule has been utilised commonly as an endogenous control in the study of microRNA expression in head and neck cancer [60,75,76], as well as squamous cell carcinoma at other sites such as the lung [77].…”

Section: Selection Of Micrornas For Further Validationmentioning

confidence: 99%

Evaluating cellular microRNA expression in human papillomavirus associated head and neck squamous cell carcinoma

Emmett¹

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Squamous cell carcinoma of mucosal head and neck sites (HNSCC) is the sixth most common cause of cancer world-wide, and is associated with a significant morbidity and mortality despite advances in therapeutic options over the past decades. HNSCC includes tumours of the oral cavity, oropharynx, larynx and hypopharynx. While there has been a decline in tumours occurring as a result of exposure to the traditional risk factors of tobacco smoking and alcohol consumption, a significant increase has been observed in the number of tumours attributed to the Human Papillomavirus (HPV). This has primarily been associated with causation of tumours in the oropharynx, however, a smaller number of tumours at other subsites are also likely to be related to the virus. Although HPV-related tumours tend to have an improved prognosis and a better response to chemo-and radiotherapy, the pathobiology underlying these differences is not yet fully understood.MiRNAs are short, non-coding single strands of RNA which regulate gene expression at the posttranscriptional level. They have been identified to be dysregulated and function as tumour suppressor or oncogenes in a number of human cancers, including head and neck cancers. MiRNAs have also been associated with prognostication including associations with recurrence of disease and diseasefree survival. As they are readily available across a range of body tissues including solid tissue, saliva, and plasma, these biomarkers have potential utility in relation to screening for disease diagnosis, recurrence or progression. Although there is an established difference between HPV-positive and negative tumours in the oropharynx clinically, the majority of studies investigating miRNA expression in HNSCC have not taken this factor into account. In the small number of studies which have examined the effect of HPV on miRNA expression in HNSCC, there is a lack of consistency in the results described.This thesis aimed to assess the difference in miRNA expression between HPV-positive and negative tumours, through use of miRNA microarray followed by validation with real-time PCR using TaqMan assays combined with a sensitive detection method (Fluidigm HD) in a larger cohort of patients. It also investigated differences in miRNA expression in association with lifestyle factors, demographics and clinical outcomes. A set of seven miRNAs was identified to be differentially expressed between HPV-positive and negative tumours in the oropharynx. No difference in miRNA expression was observed between the two tumour cohorts at other subsites in the head and neck.Differences in miRNA expression were also observed with lifestyle factors such as smoking status and alcohol consumption. Recurrence of the primary tumour and overall disease-free survival were further associated with altered miRNA expression. The findings outlined in this thesis add to the present literature regarding differences in miRNA expression between HPV-positive and negative iii tumours in the oropharynx, and provide a basis for further...

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MicroRNA Expression Characterizes Oligometastasis(es)

Cited by 253 publications

References 55 publications

miR-200–containing extracellular vesicles promote breast cancer cell metastasis

miR-200–containing extracellular vesicles promote breast cancer cell metastasis

Testing Radical prostatectomy in men with oligoMetastatic prostate cancer that has spread to the bone

Evaluating cellular microRNA expression in human papillomavirus associated head and neck squamous cell carcinoma

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