MicroRNA expression and analysis of immune-related putative target genes in ISKNV-infected spleen of mandarin fish (Siniperca chuatsi)

Zhou, Yu; Wang, Yingying; Fu, Huang-Cui; Huang, He-Zhong

doi:10.1016/j.aquaculture.2021.737450

Cited by 7 publications

(5 citation statements)

References 64 publications

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“…The spleen is the home of different types of immune cells that trigger different immune responses [ 89 , 90 , 91 ]. Splenic miRNAs have been identified to modulate immune responses during diseases in humans, mice, chickens, dogs, and fishes [ 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 ]. Our miRDeep analysis identified 268 conserved mature miRNAs in the lumpfish spleen.…”

Section: Discussionmentioning

confidence: 99%

Characterization of miRNAs in Embryonic, Larval, and Adult Lumpfish Provides a Reference miRNAome for Cyclopterus lumpus

Chakraborty

Woldemariam

Visnovska

et al. 2022

Biology

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MicroRNAs (miRNAs) are endogenous small RNA molecules involved in the post-transcriptional regulation of protein expression by binding to the mRNA of target genes. They are key regulators in teleost development, maintenance of tissue-specific functions, and immune responses. Lumpfish (Cyclopterus lumpus) is becoming an emergent aquaculture species as it has been utilized as a cleaner fish to biocontrol sea lice (e.g., Lepeophtheirus salmonis) infestation in the Atlantic Salmon (Salmo salar) aquaculture. The lumpfish miRNAs repertoire is unknown. This study identified and characterized miRNA encoding genes in lumpfish from three developmental stages (adult, embryos, and larvae). A total of 16 samples from six different adult lumpfish organs (spleen, liver, head kidney, brain, muscle, and gill), embryos, and larvae were individually small RNA sequenced. Altogether, 391 conserved miRNA precursor sequences (discovered in the majority of teleost fish species reported in miRbase), eight novel miRNA precursor sequences (so far only discovered in lumpfish), and 443 unique mature miRNAs were identified. Transcriptomics analysis suggested organ-specific and age-specific expression of miRNAs (e.g., miR-122-1-5p specific of the liver). Most of the miRNAs found in lumpfish are conserved in teleost and higher vertebrates, suggesting an essential and common role across teleost and higher vertebrates. This study is the first miRNA characterization of lumpfish that provides the reference miRNAome for future functional studies.

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Section: Discussionmentioning

confidence: 99%

Characterization of miRNAs in Embryonic, Larval, and Adult Lumpfish Provides a Reference miRNAome for Cyclopterus lumpus

Chakraborty

Woldemariam

Visnovska

et al. 2022

Biology

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“…Pathogenic mi-croorganisms can be transported to lysosomes to be killed and degraded by various acids after autophagy or endocytosis. 44,45 In addition to mediating the elimination of pathogens and harmful substances, lysosomes can still efficiently repair damaged plasma membranes during bacterial infections. 46,47 This research also found that inflammatory bowel disease (IBD) expression levels dramatically increased.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Cultured Hybrid Grouper (♀Epinephelus fuscoguttatus×♂E. lanceolatus) Immunized with Vibrio harveyi formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide

Wan,

Da,

Lin

et al. 2023

Israeli Journal of Aquaculture - Bamidgeh

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Grouper has become an essential mariculture species in China, while vibriosis caused by Vibrio harveyi significantly impacts its culture. Our previous study confirmed the V. harveyi formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide (FKC+COS) effectively prevents vibriosis. As an adjuvant, COS could significantly enhance FKC effectiveness against V. harveyi in grouper. In the present study, we performed transcriptome analysis of grouper spleens tissue 14 days post-immunization of PBS and FKC+COS, respectively. After assembly and annotation, 2,503 differentially expressed genes (DEGs) were obtained, including the upregulated 1,894 DEGs and downregulated 609 DEGs between the PBS group and FKC+COS group. To explore the relevance of DEGs in immunity, enrichment analysis in the KEGG database revealed that the main pathways of DEGs distribution associated with immunity were antigen processing and presentation, lysosome, the intestinal immune network for IgA production and FcγR-mediated phagocytosis. In conclusion, transcriptome analysis of spleens was performed to explore the potential mechanism of COS as an adjuvant enhancing the protection effectiveness of FKC against vibriosis in grouper.

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“…Recently, many studies have revealed through transcriptomic analysis that various miRNAs are significantly expressed in aquatic organisms in response to infectious diseases. [30][31][32][33][34]. For example, miRNAs regulations have been revealed in pathogenic protozoan parasites Ectoparasitic dinoflagellate Amyloodinium ocellatum infected golden pompano (Trachinotus ovatus) [30].…”

Section: Microrna Regulation In Aquaculture Responses To Infectious D...mentioning

confidence: 99%

“…The findings by Zhou's group suggested that the miRNAs could regulate autophagy during infectious spleen and kidney necrosis virus (ISKNV) infection in mandarin fish [32]. Autophagy is the natural self-degradation of damaged cells or dysfunctional components via a lysosome-dependent mechanism.…”

Section: Autophagymentioning

confidence: 99%

“…Liu et al (2015) found that the autophagy activity was upregulated during spring viraemia of carp virus (SVCV) infection and improved the survival rate of infected cells [39]. On the other hand, Zhou et al (2022) revealed that several miRNAs could regulate autophagy-related genes (Atg) and affect the autophagy activity in ISKNV-infected mandarin fish [32]. For example, novel_miR-61 targets Atg7, and novel_miR-205 and novel_miR-320 target Atg16 genes are upregulated in ISKNV-infected mandarin fish at an early stage (12 and 24 hours post-infection).…”

Section: Autophagymentioning

confidence: 99%

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MicroRNA Regulation in Infectious Diseases and Its Potential as a Biosensor in Future Aquaculture Industry: A Review

SiouNing,

Seong,

Kondo

et al. 2023

Molecules

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An infectious disease is the most apprehensive problem in aquaculture as it can lead to high mortality in aquatic organisms and massive economic loss. Even though significant progress has been accomplished in therapeutic, prevention, and diagnostic using several potential technologies, more robust inventions and breakthroughs should be achieved to control the spread of infectious diseases. MicroRNA (miRNA) is an endogenous small non-coding RNA that post-transcriptionally regulates the protein-coding genes. It involves various biological regulatory mechanisms in organisms such as cell differentiation, proliferation, immune responses, development, apoptosis, and others. Furthermore, an miRNA also acts as a mediator to either regulate host responses or enhance the replication of diseases during infection. Therefore, the emergence of miRNAs could be potential candidates for the establishment of diagnostic tools for numerous infectious diseases. Interestingly, studies have revealed that miRNAs can be used as biomarkers and biosensors to detect diseases, and can also be used to design vaccines to attenuate pathogens. This review provides an overview of miRNA biogenesis and specifically focuses on its regulation during infection in aquatic organisms, especially on the host immune responses and how miRNAs enhance the replication of pathogens in the organism. In addition to that, we explored the potential applications, including diagnostic methods and treatments, that can be employed in the aquaculture industry.

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MicroRNA expression and analysis of immune-related putative target genes in ISKNV-infected spleen of mandarin fish (Siniperca chuatsi)

Cited by 7 publications

References 64 publications

Characterization of miRNAs in Embryonic, Larval, and Adult Lumpfish Provides a Reference miRNAome for Cyclopterus lumpus

Characterization of miRNAs in Embryonic, Larval, and Adult Lumpfish Provides a Reference miRNAome for Cyclopterus lumpus

Transcriptome Analysis of the Cultured Hybrid Grouper (♀Epinephelus fuscoguttatus×♂E. lanceolatus) Immunized with Vibrio harveyi formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide

MicroRNA Regulation in Infectious Diseases and Its Potential as a Biosensor in Future Aquaculture Industry: A Review

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