MicroRNA detection on microsensor arrays by SPR imaging measurements with enzymatic signal enhancement

Aoki, Hiroshi; Corn, Robert M.; Matthews, Brandon M.

doi:10.1016/j.bios.2019.111565

Cited by 38 publications

(22 citation statements)

References 55 publications

(43 reference statements)

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“…Most miRNA genes exist in the genome as a single copy, multiple copies, or in a cluster [7]. miRNAs play important roles in various cellular functions, such as proliferation, invasion, and migration [8]. Furthermore, they have been reported to be closely associated with many kinds of tumors including glioma [9].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-346 inhibits the growth of glioma by directly targeting NFIB

Zhang

et al. 2019

Cancer Cell Int

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BackgroundGlioma is considered one of the most common tumors and has a poor prognosis. Recently, microRNAs (miRNAs) have been reported to be strongly linked to various human tumors including glioma. In this study, we investigated a new anticancer miRNA, miR-346, to determine the effects and mechanism of miR-346 and its downstream target gene NFIB on tumors.MethodsLentivirus transfection, real-time PCR, western blotting, immunohistochemistry, cell proliferation assays, and mouse experiments were used to examine the relationship between miR-346 and its regulation of NFIB in glioma cells.ResultsThe expression of miR-346 was downregulated in glioma cells. Overexpression of miR-346 arrested the cell cycle of glioma cells and inhibited their proliferation in vitro and in vivo. NFIB was a direct target of miR-346, whose expression was reduced by the miRNA. Overexpression of NFIB reversed all tested functions of miR-346.ConclusionmiR-346 inhibited the growth of glioma cells by targeting NFIB and may be a new prognostic and diagnostic biomarker for glioma.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-346 inhibits the growth of glioma by directly targeting NFIB

Zhang

et al. 2019

Cancer Cell Int

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“…(10,25,26) However, simple, rapid, and "one-time" disposable devices to avoid false positive results without any labeling of targets or any external indicators are required for reliable oligonucleotide detection in the environmental and biomedical fields. (17,27) Our technique might contribute to developing practical oligonucleotide sensor array chips that can make environmental and biomedical diagnoses simpler and easier to perform.…”

Section: Discussionmentioning

confidence: 99%

Nonlabeling and Nonexternal Indicator DNA Sensing Based on Ferrocene-terminated Probes Immobilized on Gold Film Electrode Arrays with Plasma and Acid Treatments

Aoki¹,

Sukegawa²,

Takasu³

et al. 2020

Sensors and Materials

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We fabricated a novel sensor array chip that enables simple, rapid, and sequence-specific DNA detection without any process to label DNAs or any external electrochemical indicators. We found the conditions for the cleaning of electrode surfaces and the preparation of DNA sensor array chips. A long-term storage for several weeks inactivated photolithographically fabricated 384-ch microelectrode array chips before surface modification. We revealed that the treatments with O 2 plasma and acidic solutions reactivated the long-term stored chips, and demonstrated that the DNA sensor arrays prepared from the reactivated chips detected DNAs sequence-specifically, similarly to freshly fabricated sensors. We found that feasible DNA sensor array chips can be prepared from the electrode array chips stored even for a long period, as long as the stored chips are treated before use with O 2 plasma and acid solutions under optimized conditions.

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“…Attributed to the sensitive signal transducing, SPR and SERS techniques have been adapted towards EV RNA evaluation. In fact, SPR based RNA biosensors have been developed with a variety of sophisticated plasmonic probes and signal transduction techniques (Aoki et al., 2019; Coutinho & Somoza, 2019; Fong & Yung, 2013; Xue et al., 2019), and such methods were applied for analyzing assorted biological samples, for instance, urinary miRNA (Yeung et al., 2018) and Zika viral RNA (Adegoke et al., 2017). However, few published investigations have focused on EV RNA characterization.…”

Section: Emerging Methods In Ev Assessmentmentioning

confidence: 99%

Emerging methods in biomarker identification for extracellular vesicle‐based liquid biopsy

Liang

Lehrich

Zheng

et al. 2021

J of Extracellular Vesicle

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Extracellular vesicles (EVs) are released by many cell types and distributed within various biofluids. EVs have a lipid membrane-confined structure that allows for carrying unique molecular information originating from their parent cells. The species and quantity of EV cargo molecules, including nucleic acids, proteins, lipids, and metabolites, may vary largely owing to their parent cell types and the pathophysiologic status. Such heterogeneity in EV populations provides immense challenges to researchers, yet allows for the possibility to prognosticate the pathogenesis of a particular tissue from unique molecular signatures of dispersing EVs within biofluids. However, the inherent nature of EV's small size requires advanced methods for EV purification and evaluation from the complex biofluid. Recently, the interdisciplinary significance of EV research has attracted growing interests, and the EV analytical platforms for their diagnostic prospect have markedly progressed. This review summarizes the recent advances in these EV detection techniques and methods with the intention of translating an EV-based liquid biopsy into clinical practice. This article aims to present an overview of current EV assessment techniques, with a focus on their progress and limitations, as well as an outlook on the clinical translation of an EV-based liquid biopsy that may augment current paradigms for the diagnosis, prognosis, and monitoring the response to therapy in a variety of disease settings.

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MicroRNA detection on microsensor arrays by SPR imaging measurements with enzymatic signal enhancement

Cited by 38 publications

References 55 publications

MicroRNA-346 inhibits the growth of glioma by directly targeting NFIB

MicroRNA-346 inhibits the growth of glioma by directly targeting NFIB

Nonlabeling and Nonexternal Indicator DNA Sensing Based on Ferrocene-terminated Probes Immobilized on Gold Film Electrode Arrays with Plasma and Acid Treatments

Emerging methods in biomarker identification for extracellular vesicle‐based liquid biopsy

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