MicroRNA delivery through nanoparticles

Lee, Sharon Wei Ling; Paoletti, Camilla; Campisi, Marco; Osaki, Tatsuya; Adriani, Giulia; Kamm, Roger D.; Mattu, Clara; Chiono, Valeria

doi:10.1016/j.jconrel.2019.10.007

Cited by 254 publications

(185 citation statements)

References 241 publications

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“…These vector-based systems can be sub-divided into two further classes: (1) Viral [72] and (2) non-viral [73] systems. Viral and non-viral approaches used in RNA-based therapies have reviewed extensively within the literature [7,74,75].…”

Section: Mechanisms Of Mirna Delivery For Bone Repairmentioning

confidence: 99%

“…The use of calcium phosphate nanoparticles as non-viral delivery vectors has also been shown to promote enhanced osteogenic differentiation of bone marrow-derived MSCs compared to PEI [115]. Furthermore, calcium phosphate-based powders are inexpensive and easily synthesized nanocarriers and a high level of safety relating to the use of calcium phosphate nanoparticles for cell transfection has been reported [74,75,116].…”

Section: Calcium Phosphates Nanoparticles As Non-viral Vectorsmentioning

confidence: 99%

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Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

et al. 2020

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Bone-related injury and disease constitute a significant global burden both socially and economically. Current treatments have many limitations and thus the development of new approaches for bone-related conditions is imperative. Gene therapy is an emerging approach for effective bone repair and regeneration, with notable interest in the use of RNA interference (RNAi) systems to regulate gene expression in the bone microenvironment. Calcium phosphate nanoparticles represent promising materials for use as non-viral vectors for gene therapy in bone tissue engineering applications due to their many favorable properties, including biocompatibility, osteoinductivity, osteoconductivity, and strong affinity for binding to nucleic acids. However, low transfection rates present a significant barrier to their clinical use. This article reviews the benefits of calcium phosphate nanoparticles for RNAi delivery and highlights the role of surface functionalization in increasing calcium phosphate nanoparticles stability, improving cellular uptake and increasing transfection efficiency. Currently, the underlying mechanistic principles relating to these systems and their interplay during in vivo bone formation is not wholly understood. Furthermore, the optimal microRNA targets for particular bone tissue regeneration applications are still unclear. Therefore, further research is required in order to achieve the optimal calcium phosphate nanoparticles-based systems for RNAi delivery for bone tissue regeneration.

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Section: Mechanisms Of Mirna Delivery For Bone Repairmentioning

confidence: 99%

Section: Calcium Phosphates Nanoparticles As Non-viral Vectorsmentioning

confidence: 99%

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

et al. 2020

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“…Since nanoparticles offer incredible opportunities for cell specific controlled delivery of miRNAs in the treatment of cancer or neurodegenerative disorders [57], our findings thus provide a potential therapeutic strategy for cancer, such as breast cancer. Moreover, inhibition of miR-218 (e.g., AntagomiR) is expected to be beneficial in several neurodegenerative diseases in which the mitophagy process is not sufficiently efficient.…”

Section: Discussionmentioning

confidence: 96%

miR-218 Inhibits Mitochondrial Clearance by Targeting PRKN E3 Ubiquitin Ligase

Rita

Maiorino

Bruqi

et al. 2020

IJMS

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The selective elimination of dysfunctional mitochondria through mitophagy is crucial for preserving mitochondrial quality and cellular homeostasis. The most described mitophagy pathway is regulated by a positive ubiquitylation feedback loop in which the PINK1 (PTEN induced kinase 1) kinase phosphorylates both ubiquitin and the E3 ubiquitin ligase PRKN (Parkin RBR E3 ubiquitin ligase), also known as PARKIN. This event recruits PRKN to the mitochondria, thus amplifying ubiquitylation signal. Here we report that miR-218 targets PRKN and negatively regulates PINK1/PRKN-mediated mitophagy. Overexpression of miR-218 reduces PRKN mRNA levels, thus also reducing protein content and deregulating the E3 ubiquitin ligase action. In fact, following miR-218 overexpression, mitochondria result less ubiquitylated and the autophagy machinery fails to proceed with correct mitochondrial clearance. Since mitophagy defects are associated with various human diseases, these results qualify miR-218 as a promising therapeutic target for human diseases.

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“…Nanoparticle-based delivery has been reported to tackle these above-mentioned obstacles related to miRNA therapeutics [72,73]. Nanotherapeutics are one of the most favorable platforms to effectively deliver the miRNAs to the host cells pertaining to their tiny size and low molecular weight.…”

Section: Therapeutic Potential Of Mirnas For Covid-19mentioning

confidence: 99%

COVID-19: fighting the invisible enemy with microRNAs

Chauhan

Jaggi

Chauhan

et al. 2020

Expert Review of Anti-infective Therapy

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Introduction: The novel coronavirus (CoV) disease 2019 (COVID-19) is a viral infection that causes severe acute respiratory syndrome (SARS). It is believed that early reports of COVID-19 cases were noticed in December 2019 and soon after it became a global public health emergency. It is advised that COVID-19 transmits through human to human contact and in most cases, it remains asymptomatic. Several approaches are being utilized to control the outbreak of this fatal viral disease. microRNAs (miRNAs) are known signature therapeutic tool for the viral diseases; they are small non-coding RNAs that target the mRNAs to inhibit their post-transcriptional expression, therefore, impeding their functions, can serve as watchdogs or micromanagers in the cells. Areas covered: This review work delineated COVID-19 and its association with SARS and Middle East respiratory syndrome (MERS), the possible role of miRNAs in the pathogenesis of COVID-19, and therapeutic potential of miRNAs and their effective delivery to treat COVID-19. Expert opinion: This review highlighted the importance of various miRNAs and their potential role in fighting with this pandemic as therapeutic molecules utilizing nanotechnology.

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MicroRNA delivery through nanoparticles

Cited by 254 publications

References 241 publications

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

miR-218 Inhibits Mitochondrial Clearance by Targeting PRKN E3 Ubiquitin Ligase

COVID-19: fighting the invisible enemy with microRNAs

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