MicroRNA-based transcriptomic responses of Atlantic salmon during infection by the intracellular bacterium Piscirickettsia salmonis

Valenzuela-Miranda, Diego; Valenzuela-Muñoz, Valentina; Farlora, Rodolfo; Gallardo‐Escárate, Cristian

doi:10.1016/j.dci.2017.08.016

Cited by 27 publications

(20 citation statements)

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“…They identified 20 differentially expressed miRNAs that may be important in viral-host interactions in Atlantic salmon. Following the same field of interest, Valenzuela-Miranda et al ( 2017 ) studied the expression of miRNAs during infection with Piscirickettsia salmonis in the head, kidney and spleen. Piscirickettsia salmonis , a facultative intracellular bacterium, causes salmonid rickettsial septicemia (SRS), which is associated with major mortality in salmonid aquaculture (Mauel and Miller, 2002 ).…”

Section: A Review Of Mirnas In Farmed Fishesmentioning

confidence: 99%

“…Piscirickettsia salmonis , a facultative intracellular bacterium, causes salmonid rickettsial septicemia (SRS), which is associated with major mortality in salmonid aquaculture (Mauel and Miller, 2002 ). The miRNA families miR-181, miR-143, and miR-21 were the most abundant in control groups, while miR-21, miR-181, and miR-30 were the most abundant in animals infected with P. salmonis (Valenzuela-Miranda et al, 2017 ). Sea louse Caligus rogercresseyi , which affects Chilean aquaculture, were studied during infestation in Atlantic salmon and the most abundant families were mir-10, mir-21, mir-30, mir-181, and let7 in skin, head and kidney (Valenzuela-Muñoz et al, 2017 ).…”

Section: A Review Of Mirnas In Farmed Fishesmentioning

confidence: 99%

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Fishing Into the MicroRNA Transcriptome

et al. 2018

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In the last decade, several studies have been focused on revealing the microRNA (miRNA) repertoire and determining their functions in farm animals such as poultry, pigs, cattle, and fish. These small non-protein coding RNA molecules (18–25 nucleotides) are capable of controlling gene expression by binding to messenger RNA (mRNA) targets, thus interfering in the final protein output. MiRNAs have been recognized as the main regulators of biological features of economic interest, including body growth, muscle development, fat deposition, and immunology, among other highly valuable traits, in aquatic livestock. Currently, the miRNA repertoire of some farmed fish species has been identified and characterized, bringing insights about miRNA functions, and novel perspectives for improving health and productivity. In this review, we summarize the current advances in miRNA research by examining available data on Neotropical and other key species exploited by fisheries and in aquaculture worldwide and discuss how future studies on Neotropical fish could benefit from this knowledge. We also make a horizontal comparison of major results and discuss forefront strategies for miRNA manipulation in aquaculture focusing on forward-looking ideas for forthcoming research.

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Section: A Review Of Mirnas In Farmed Fishesmentioning

confidence: 99%

Section: A Review Of Mirnas In Farmed Fishesmentioning

confidence: 99%

Fishing Into the MicroRNA Transcriptome

et al. 2018

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“…Of the 4 miRNAs identified by deep sequencing as being responsive only to pIC, 3 (i.e., miR-30e-1-2-3p, miR-181a-5-3p, and miR-462a-3p) were qPCR confirmed as being significantly up-regulated in fish fed the control diet. Although there have been no studies to date reporting the association of miR-30e with antiviral immune response in Atlantic salmon, a previous study in Atlantic salmon infected with the intracellular bacterium P. salmonis did show increased miR-30e expression in both spleen and head kidney [23]. miR-181 and miR-462 were classified as evolutionarily conserved miRNAs associated with immune response in teleosts following viral challenges [18].…”

Section: Mirnas Only Associated With Antiviral Immune Response In Atlmentioning

confidence: 99%

“…Recent advances in high-throughput sequencing technology (e.g., small RNA deep sequencing) and bioinformatics tools have led to the detection of virus/bacteria-responsive miRNAs in teleosts [19,21,23,24]. For instance, twenty differentially expressed miRNAs were identified in Atlantic salmon challenged with SAV; the majority of the predicted mRNA targets were involved in promoting the inflammatory response [19].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, twenty differentially expressed miRNAs were identified in Atlantic salmon challenged with SAV; the majority of the predicted mRNA targets were involved in promoting the inflammatory response [19]. Analyses of Atlantic salmon tissues infected with P. salmonis revealed 84 and 25 differentially expressed miRNAs in head kidney and spleen, respectively; functional annotation of predicted mRNA targets of P. salmonis-responsive miRNAs showed involvement in the immune response, such as genes related to chemokine-mediated signaling pathway and neutrophil chemotaxis [23]. Such studies have improved our understanding of miRNAs involved in immune responses in teleosts [18].…”

Section: Introductionmentioning

confidence: 99%

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Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

Xue

Woldemariam

Caballero‐Solares

et al. 2019

Cells

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MicroRNAs (miRNAs) are key regulators in fish immune responses. However, no study has previously characterized the impact of polyriboinosinic polyribocytidylic acid (pIC) and formalin-killed typical Aeromonas salmonicida (ASAL) on miRNA expression in Atlantic salmon fed a commercial diet with and without immunostimulant CpG. To this end, first, we performed small RNA deep sequencing and qPCR analyses to identify and confirm pIC- and/or ASAL-responsive miRNAs in the head kidney of salmon fed a control diet. DESeq2 analyses identified 12 and 18 miRNAs differentially expressed in pIC and ASAL groups, respectively, compared to the controls. Fifteen of these miRNAs were studied by qPCR; nine remained significant by qPCR. Five miRNAs (miR-27d-1-2-5p, miR-29b-2-5p, miR-146a-5p, miR-146a-1-2-3p, miR-221-5p) were shown by qPCR to be significantly induced by both pIC and ASAL. Second, the effect of CpG-containing functional feed on miRNA expression was investigated by qPCR. In pre-injection samples, 6 of 15 miRNAs (e.g., miR-181a-5-3p, miR-462a-3p, miR-722-3p) had significantly lower expression in fish fed CpG diet than control diet. In contrast, several miRNAs (e.g., miR-146a-1-2-3p, miR-192a-5p, miR-194a-5p) in the PBS- and ASAL-injected groups had significantly higher expression in CpG-fed fish. Multivariate statistical analyses confirmed that the CpG diet had a greater impact on miRNA expression in ASAL-injected compared with pIC-injected fish. This study identified immune-relevant miRNA biomarkers that will be valuable in the development of diets to combat infectious diseases of salmon.

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Development of Epigenetic Biomarkers in Aquatic Organisms

Anastasiadi

Beemelmanns

2023

Epigenetics in Aquaculture

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MicroRNA-based transcriptomic responses of Atlantic salmon during infection by the intracellular bacterium Piscirickettsia salmonis

Cited by 27 publications

References 59 publications

Fishing Into the MicroRNA Transcriptome

Fishing Into the MicroRNA Transcriptome

Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

Development of Epigenetic Biomarkers in Aquatic Organisms

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