microRNA and Wound Healing

Banerjee, Jaideep; Sen, Chandan K.

doi:10.1007/978-3-319-22671-2_15

Cited by 54 publications

(46 citation statements)

References 65 publications

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“…Both lncRNAs and miRNAs are potential biomarkers for diagnosis and prognosis of wound healing treatment. For instance, miR-210 serves as a novel molecular marker for chronic wound healing outcomes in a clinical trial 36 . miRNA-based treatment is more effective compared with traditional treatment 37 .…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of long non-coding RNA HOTAIR promotes angiogenesis via regulating miR-126/SCEL pathways in burn wound healing

et al. 2020

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miR-126, an endothelial-specific microRNA, is associated to vascular integrity and angiogenesis. It is well established that angiogenesis plays a critical role in burn wound healing. However, there was a lack of understanding of the mechanism by which miR-126 regulates angiogenesis during burn wound healing. HOX transcript antisense intergenic RNA (HOTAIR) is a well-characterized long non-coding RNA (lncRNA) involved in cell proliferation, apoptosis, migration, and invasion of cancer cells. Sciellin (SCEL), a precursor to the cornified envelope of human keratinocytes, has been shown to inhibit migration and invasion capabilities of colorectal cancer cells. In this study, a cohort of 20 burn wound tissues and paired adjacent normal tissues were collected. LncRNA and messenger RNA expression profiles were screened by microarray analysis in five pairs of samples with mostly increased miR-126 levels. miR-126 was highly expressed in burn wound tissues and human umbilical vein endothelial cells (HUVECs) exposed to heat stress, whereas HOTAIR and SCEL were down-regulated after thermal injury. Bioinformatic analysis, dual luciferase reporter assay, and quantitative real-time PCR were conducted to validate that HOTAIR and SCEL competitively bind to miR-126 to function as the competitive endogenous RNA. miR-126 promoted endothelial cell proliferation, migration, and angiogenesis, but suppressed apoptosis, while HOTAIR and SCEL exerted opposite effects in HUVECs. The biological functions were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, Annexin-V-FITC/PI (propidium iodide/fluorescein isothiocyanate) staining, transwell migration, and tube formation assays. Collectively, our study revealed that HOTAIR/miR-126/SCEL axis contributes to burn wound healing through mediating angiogenesis.

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Section: Discussionmentioning

confidence: 99%

Down-regulation of long non-coding RNA HOTAIR promotes angiogenesis via regulating miR-126/SCEL pathways in burn wound healing

et al. 2020

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“…The role of miRNAs and their impact on microvascular complications has been excellently covered elsewhere (Moura et al . , Banerjee & Sen , Bhatt et al . ).…”

Section: Muscle and Heart Mirnasmentioning

confidence: 99%

MicroRNAs in metabolism

et al. 2016

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MicroRNAs (miRNAs) have within the past decade emerged as key regulators of metabolic homoeostasis. Major tissues in intermediary metabolism important during development of the metabolic syndrome, such as β‐cells, liver, skeletal and heart muscle as well as adipose tissue, have all been shown to be affected by miRNAs. In the pancreatic β‐cell, a number of miRNAs are important in maintaining the balance between differentiation and proliferation (miR‐200 and miR‐29 families) and insulin exocytosis in the differentiated state is controlled by miR‐7, miR‐375 and miR‐335. MiR‐33a and MiR‐33b play crucial roles in cholesterol and lipid metabolism, whereas miR‐103 and miR‐107 regulates hepatic insulin sensitivity. In muscle tissue, a defined number of miRNAs (miR‐1, miR‐133, miR‐206) control myofibre type switch and induce myogenic differentiation programmes. Similarly, in adipose tissue, a defined number of miRNAs control white to brown adipocyte conversion or differentiation (miR‐365, miR‐133, miR‐455). The discovery of circulating miRNAs in exosomes emphasizes their importance as both endocrine signalling molecules and potentially disease markers. Their dysregulation in metabolic diseases, such as obesity, type 2 diabetes and atherosclerosis stresses their potential as therapeutic targets. This review emphasizes current ideas and controversies within miRNA research in metabolism.

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“…On the other hand, non-viral miRNA delivery systems are characterized by lower toxicity and immunogenicity, increased cellular uptake, water solubility, resistance to endonucleases, and phagocytosis [385,387]. specificity [403,404]. Nonetheless, in order to design a safe and efficient miRNA nanocarrier some concerns must be considered [403].…”

Section: Microrna -Ecm Interactions In Wound Healingmentioning

confidence: 99%

“…specificity [403,404]. Nonetheless, in order to design a safe and efficient miRNA nanocarrier some concerns must be considered [403]. The most important issue is the anatomy of the targeted organ, however, the therapeutic dose, the tissue microenvironment and the ECM composition of each cell type must be evaluated in order to improve the therapeutic potential of the mimic miRNA or the anti-miRNA.…”

Section: Microrna -Ecm Interactions In Wound Healingmentioning

confidence: 99%

Insights into the key roles of epigenetics in matrix macromolecules-associated wound healing

Piperigkou

Götte

Theocharis

et al. 2018

Advanced Drug Delivery Reviews

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Extracellular matrix (ECM) is a dynamic network of macromolecules, playing a regulatory role in cell functions, tissue regeneration and remodeling. Wound healing is a tissue repair process necessary for the maintenance of the functionality of tissues and organs. This highly orchestrated process is divided into four temporally overlapping phases, including hemostasis, inflammation, proliferation and tissue remodeling. The dynamic interplay between ECM and resident cells exerts its critical role in many aspects of wound healing, including cell proliferation, migration, differentiation, survival, matrix degradation and biosynthesis. Several epigenetic regulatory factors, such as the endogenous non-coding microRNAs (miRNAs), are the drivers of the wound healing response. microRNAs have pivotal roles in regulating ECM composition during wound healing and dermal regeneration. Their expression is associated with the distinct phases of wound healing and they serve as target biomarkers and targets for systematic regulation of wound repair. In this article we critically present the importance of epigenetics with particular emphasis on miRNAs regulating ECM components (i.e. glycoproteins, proteoglycans and matrix proteases) that are key players in wound healing. The clinical relevance of miRNA targeting as well as the delivery strategies designed for clinical applications are also presented and discussed.

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microRNA and Wound Healing

Cited by 54 publications

References 65 publications

Down-regulation of long non-coding RNA HOTAIR promotes angiogenesis via regulating miR-126/SCEL pathways in burn wound healing

Down-regulation of long non-coding RNA HOTAIR promotes angiogenesis via regulating miR-126/SCEL pathways in burn wound healing

MicroRNAs in metabolism

Insights into the key roles of epigenetics in matrix macromolecules-associated wound healing

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