MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

Korvala, Johanna; Jee, Kowan Ja; Porkola, Emmi; Almangush, Alhadi; Mosakhani, Neda; Bitu, Carolina Cavalcante; Cervigne, Nilva K.; Zandonadi, Flávia S.; Meirelles, Gabriela Vaz; Leme, Adriana Franco Paes; Coletta, Ricardo D.; Leivo, Ilmo; Salo, Tuula

doi:10.1016/j.yexcr.2016.10.015

Cited by 17 publications

(17 citation statements)

References 63 publications

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“…The reduced expression and tumor-suppressive role of miR-498 have been found in esophageal squamous cell carcinoma [ 20 ], colorectal cancer [ 21 ], non-small cell lung cancer [ 22 ], and ovarian cancer [ 23 ]. However, in triple negative breast cancer [ 24 ] and oral tongue squamous cell carcinoma [ 25 ], miR-498 has been shown to play promoting roles in cancer cell proliferation and invasion, suggesting that the functions of miR-498 may be cancer type-specific and cell context-dependent. In this study, we demonstrated that miR-498 expression was downregulated in gastric cancer and low level of miR-498 predicted poor prognosis for patients with gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA UFC1 promotes gastric cancer progression by regulating miR-498/Lin28b

Zhang

Liang

Liu

et al. 2018

J Exp Clin Cancer Res

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BackgroundLong non-coding RNAs (lncRNAs) have emerged as important regulators of human cancers. However, the functional roles of lncRNAs and the mechanisms responsible for their aberrant expression in gastric cancer (GC) have not been well characterized.MethodsIn this study, we examined the expression of lncRNA UFC1 in GC by qRT-PCR and explored its correlation with clinicopathological parameters. In vitro cell functional assays and in vivo animal studies were performed to determine the roles of UFC1 in GC progression.ResultsUFC1 was elevated and predicted poorer prognosis in GC. UFC1 knockdown inhibited while UFC1 overexpression promoted GC cell proliferation, migration, and invasion. UFC1 bound to miR-498 to antagonize its tumor suppressive effect on Lin28b. Suppression of Lin28b by miR-498 could be rescued by UFC1 overexpression, whereas Lin28b overexpression partially rescued UFC1 knockdown-mediated inhibition of GC cell function. Lin28b expression was increased in GC and suggested a co-expression pattern with UFC1.ConclusionsUFC1 has a promoting role in GC progression, at least in part, by acting as a miR-498 sponge and derepressing Lin28b expression, which would provide a novel biomarker for GC diagnosis and prognosis and offer a potential target for GC therapy.Electronic supplementary materialThe online version of this article (10.1186/s13046-018-0803-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Long non-coding RNA UFC1 promotes gastric cancer progression by regulating miR-498/Lin28b

Zhang

Liang

Liu

et al. 2018

J Exp Clin Cancer Res

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“…Myoma discs are used to compare the invasion of parental and transduced cell lines, such as MMP-8, endostatin, urokinase plasminogen activator receptor (uPAR) and trypsin-2 overexpressed, or cathepsin K, snail, miRNA-498 and miRNA-940 silenced carcinoma cell lines [ 39 , 54 , 68 , 71 , 75 – 77 ]. Similarly, several compounds have been studied for their effects on invasion.…”

Section: Three-dimensional In Vitro Invasion Assaymentioning

confidence: 99%

Organotypic three-dimensional assays based on human leiomyoma–derived matrices

Salo

Dourado

Sundquist

et al. 2017

Phil. Trans. R. Soc. B

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Alongside cancer cells, tumours exhibit a complex stroma containing a repertoire of cells, matrix molecules and soluble factors that actively crosstalk between each other. Recognition of this multifaceted concept of the tumour microenvironment (TME) calls for authentic TME mimetics to study cancer in vitro. Traditionally, tumourigenesis has been investigated in non-human, three-dimensional rat type I collagen containing organotypic discs or by means of mouse sarcoma-derived gel, such as Matrigel®. However, the molecular compositions of these simplified assays do not properly simulate human TME. Here, we review the main properties and benefits of using human leiomyoma discs and their matrix Myogel for in vitro assays. Myoma discs are practical for investigating the invasion of cancer cells, as are cocultures of cancer and stromal cells in a stiff, hypoxic TME mimetic. Myoma discs contain soluble factors and matrix molecules commonly present in neoplastic stroma. In Transwell, IncuCyte, spheroid and sandwich assays, cancer cells move faster and form larger colonies in Myogel than in Matrigel®. Additionally, Myogel can replace Matrigel® in hanging-drop and tube-formation assays. Myogel also suits three-dimensional drug testing and extracellular vesicle interactions. To conclude, we describe the application of our myoma-derived matrices in 3D in vitro cancer assays.This article is part of the discussion meeting issue ‘Extracellular vesicles and the tumour microenvironment’.

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“…Oral tongue squamous cell carcinoma (OTSCC), which is the most common and aggressive cancer of the oral cavity, is characterised by an unpredictable prognosis even at the early stage of the disease. The incidence of OTSCC has increased worldwide even in countries where tobacco use, which is the major risk factor for oral cancer, has decreased . Overall, OTSCC is associated with a high risk of occult lymph node metastasis, recurrence, and cancer‐related mortality …”

Section: Introductionmentioning

confidence: 99%

“…The incidence of OTSCC has increased worldwide even in countries where tobacco use, which is the major risk factor for oral cancer, has decreased. 1 Overall, OTSCC is associated with a high risk of occult lymph node metastasis, recurrence, and cancer-related mortality. 2,3 Treatment planning for OTSCC is generally based on disease staging, i.e.…”

Section: Introductionmentioning

confidence: 99%