MicroRNA and Cardiac Stem Cell Therapy

Serradifalco, Claudia; Zummo, Giovanni; Felice, Valentina Di

doi:10.4172/2155-9880.s11-001

Cited by 3 publications

(3 citation statements)

References 81 publications

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“…In our previous review, mentioned few cases as biomarker for cardiologists, hereby, this study summarizes the cuttingedge applications of miRNA. In a recent study by Serradifalco et al proved that nanoparticle bearing miRNAs reprogrammed CPCs in a biodegradable 3D scaffold [40,41]. Now, these molecules have great potential to understanding the pathophysciology and offer a novel class of biomarkers for acute myocardial infarction diagnosis.…”

Section: Clinical Significance Of Mirnasmentioning

confidence: 99%

MicroRNA: A Target Candidate to Turn the Tide

Rao¹

2015

Mol Biol

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Understanding the molecular basis of life is at the centre today, the integration of Computer Science, Mathematics and statistics improved the belief in structural and functional genomics. Recently there is an increased interest in function of non-coding RNA transcripts. Non-coding RNAs are small molecules that are not translated into proteins, but these molecules have a pivotal role in almost all molecular and cellular processes. MicroRNA is one of those noncoding RNA molecules that regulate gene expression at posttranslational level. These 18-25 nt length molecules are primary transcribes consist of a secondary hairpin structure. There are 700 miRNAs in the human genome, at least 1-4% of expressed genes; this makes miRNAs as the biggest group of regulators. This review summarizes biogenesis, role of expression profiles in and at various cellular and molecular mechanisms and their applications. More or less, research on non-coding RNA candidates has been done and proved their importance, however, even more to be proved.

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Section: Clinical Significance Of Mirnasmentioning

confidence: 99%

MicroRNA: A Target Candidate to Turn the Tide

Rao¹

2015

Mol Biol

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“…Regarding heart regeneration and the role of miRNAs, studies have reported that the molecules are capable to reprogramme the CSCs to attain three dimensional structure of cardiac muscle in its correct orientation. To put forth the applications of miRNAs in cardiac regeneration, recent studies have proved that nanoparticle bearing miRNAs are able to reprogramme isolated CPCs in a biodegradable tolerated 3D scaffold [19].…”

Section: Microrna As Cardiac Biomarkermentioning

confidence: 99%

MicroRNA: A New Promising Biomarker

Vd¹

2013

J Cancer Sci Ther

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Understanding the molecular basis of life is at the center today, however, advances in the field of Molecular Biology and genomics improved the compassionate of the genetic basis of diseases in human. There is an increased interest in the function of non-coding RNA transcripts in early diagnosis and treatment to various diseases. MicroRNAs are classes of small non-coding RNAs that consist of 22-25 nucleotides, which are one of the most current forms of molecular characterization of tumours. The target MicroRNA regulates the mRNA translation that causes the subsequent decrease in the protein expression. This paper aims to review some of the accumulated literature that has proven that miRNAs are strong biomarkers in diagnosis and treatment of life threatening diseases such as cancer.

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“…[5][6][7] MiRs target most human protein coding genes; especially miR-499 encoded by myosin gene family shows a high expression in circulation in patients with AMI. 8 It is involved in the structural and functional differentiation of cardiac stem cell into cardiomyocytes 9 and also plays a pivotal role in the recovery of cardiac cell, following injury. 10,11 Few studies in the recent past have shown that miR in body fl uids are highly stable and become specifi c molecular signatures for diseases thus enabling them as markers of diagnosis as well as prognosis.…”

Section: Introductionmentioning

confidence: 99%

Role of serum microRNA-499 as a diagnostic marker in acute myocardial infarction

et al. 2019

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SOUHRNAkutní infarkt myokardu je jednou z hlavních příčin morbidity a mortality v rozvojových zemích, jako např. v Indii, a dokonce po celém světě. Jeho etiologie je složitá vzhledem k několika zkreslujícím faktorům v jeho patogenezi. V poslední době se prokázalo, že významnou úlohu v predikci diagnózy a prognózy infarktu myokardu hrají malé molekuly RNA, označované jako mikroRNA (miR), z nichž miR-499 hraje zcela zásadní roli v zotavování kardiomyocytů po poranění. Od té doby několik studií identifi kovalo několik typů miR spojených s kardiovaskulárními onemocněními; z nich se miR-499 exprimuje hlavně v myokardu. Hlavním cílem naší studie bylo zkoumat úlohu miR-499 v diagnostice AIM. Do studie bylo zařazeno 60 pacientů s AIM ve věku 30 až 60 let a stejný počet kontrolních osob odpovídajícího věku a pohlaví. Zařazení všech účastníků proběhlo na pracovišti terciární péče (Mahatma Gandhi Medical College and Research Institute). Analýza hodnot miR-499 se prováděla metodou RT-PCR, zatímco lipidový profi l a hodnoty CK (NAC), CK-MB a srdečních troponinů (pomocí vysoce senzitivní metody) se stanovovaly metodami schválenými IFCC. Pro srovnání průměrných hodnot případů a kontrol byl použit nepárový Studentův test. Vztah mezi hodnotami miR a klasickými markery se zkoumal pomocí Pearsonova korelačního koefi cientu. Senzitivita a specifi cita miR-499 u AIM se hodnotila pomocí vynesené křivky ROC (Receiver Operating Characteristic). Naše studie prokázala statisticky významně vysoké hodnoty miR-499 u pacientů s AMI ve srovnání se zdravými kontrolními osobami (p = 0,012). Hodnoty miR-499 pozitivně korelovaly s hodnotami hs-cnTnT (r = 0,582; p < 0,01) a CK-MB (r = 0,443; p < 0,01). Plocha pod křivkou ROC pro miR-499 měla hodnotu 0,974 se senzitivitou 93,33 % a specifi citou 86,67 %; tedy poměrně vyššími než v případě v současnosti používaných markerů CK-MB (86,67 %, resp. 71,67 %) a hs-cnTnT (90,00 %, resp. 81,56 %). Analýza kombinované senzitivity uvedených biomarkerů prokázala, že jejich senzitivita se zvyšuje, zatímco specifi cita se snižuje. Z výsledků vyplývá, že miR-499 významně koreluje s ostatními klasickými markery při vyšší senzitivitě a specifi citě, a mohl by tedy sloužit jako přesnější marker v časné diagnostice AIM. ABSTRACTAcute myocardial infarction is one of the leading causes of morbidity and mortality in developing countries such as India and even worldwide. Its etiology is complicated because of several confounding factors involved in its pathogenesis. Recently, MicroRNA has been recognized to play an important role in predicting the diagnosis and prognosis of myocardial infarction. MiR-499 plays a pivotal role in the recovery of cardiac cell, following injury. Since several studies have profi led several miRs in cardiovascular diseases and of which miR-499 is mainly expressed in the myocardium. Our study is primarily designed to explore the diagnostic role of miR-499 in AMI. The study included 60 AMI patients aged 30-60 years and an equal number of age and gender matched controls. All cases were taken from ...

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MicroRNA and Cardiac Stem Cell Therapy

Cited by 3 publications

References 81 publications

MicroRNA: A Target Candidate to Turn the Tide

MicroRNA: A Target Candidate to Turn the Tide

MicroRNA: A New Promising Biomarker

Role of serum microRNA-499 as a diagnostic marker in acute myocardial infarction

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