microRNA, a Subtle Indicator of Human Cytomegalovirus against Host Immune Cells

Yu, Mengyao; Jin, Yang; Zhang, Shichang; Xu, Jin; Zhang, Jie-Xin

doi:10.3390/vaccines10020144

Cited by 4 publications

(2 citation statements)

References 132 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, all PSS patients who underwent glaucoma surgery developed iris depigmentation. Kam et al [ 2 ] identified iris depigmentation as a clinical biomarker for predicting chronic or recurrent anterior uveitis CMV infection in a prospective cohort study [ 6 ]. Our findings are consistent with theirs, revealing that PSS patients with glaucomatous damage not only had iris depigmentation but also increased CMV DNA copy numbers in aqueous humor compared to controls.…”

Section: Discussionmentioning

confidence: 99%

“…The diagnostic criteria for PSS were as follows [1]: (1) recurrent intermittent, acute, mild non-granulomatous anterior uveitis; (2) IOP higher than 21 mm Hg; (3) keratic precipitates on corneal endothelial surface; (4) cell and flare in the anterior chamber; (5) open anterior chamber angle; and (6) no iris posterior synechiae or posterior inflammation. Secondary glaucoma development in PSS patients was defined by the following criteria: (1) persistent elevation of IOP in the affected eye (>21 mm Hg), with a bilateral IOP difference of >8 mm Hg; and (2) glaucomatous cupping and retinal nerve fiber layer (RNFL) loss accompanied by progressive visual field defects [6][7][8].…”

Section: Patientsmentioning

confidence: 99%

See 1 more Smart Citation

Clinical Differences between Posner-Schlossman Syndrome Patients with Intermittent Intraocular Pressure Elevation and Glaucomatous Damage

Wang,

Zeng,

Zeng

et al. 2023

Ophthalmic Res

View full text Add to dashboard Cite

Introduction: Although there is abundant evidence that Posner-Schlossman syndrome (PSS) can lead to secondary glaucoma, data on the clinical differences between PSS patients with secondary glaucoma and those with intermittent intraocular pressure (IOP) elevation are sparse. Methods: This retrospective observational study included 52 patients (52 eyes) diagnosed with PSS and admitted to Zhongnan Hospital of Wuhan University between January 2019 and February 2022. Demographic characteristics and clinical features were gathered from admission records. Patients were divided into two groups: 27 cases with intermittent IOP elevation (group A) and 25 cases with secondary glaucoma (group B and C). Of the secondary glaucoma cases, 18 were further divided into the topical IOP-lowering medications group (group B) and 7 into the glaucoma surgery group (group C). Clinical characteristics of different groups were compared. Results: Compared to the intermittent IOP elevation group, PSS patients with secondary glaucoma had a longer course of disease, a higher incidence of iris depigmentation, lower best corrected visual acuity, lower endothelial cell density, and higher interferon-γ (IFN-γ) concentration and cytomegalovirus (CMV) deoxyribonucleic acid (DNA) copy number in the aqueous humor (all p < 0.05). Group C presented a higher CMV DNA copy number in the aqueous humor than groups A and B (p < 0.05). Compound trabeculectomy proved effective in group C, with a functional filter bleb and well-controlled IOP without disease progression after 1 year of follow-up. Conclusion: Distinctive characteristics existed between PSS patients with secondary glaucoma and those with intermittent IOP elevation. Compound trabeculectomy appears to be an effective treatment option when IOP cannot be controlled through topical medications.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Patientsmentioning

confidence: 99%

Clinical Differences between Posner-Schlossman Syndrome Patients with Intermittent Intraocular Pressure Elevation and Glaucomatous Damage

Wang,

Zeng,

Zeng

et al. 2023

Ophthalmic Res

View full text Add to dashboard Cite

show abstract

Human cytomegalovirus microRNAs: strategies for immune evasion and viral latency

Sabbaghian,

Gheitasi,

Fadaee

et al. 2024

Arch Virol

View full text Add to dashboard Cite

Bursal-Derived BP7 Induces the miRNA Molecular Basis of Chicken Macrophages and Promotes the Differentiation of B Cells

Cai

Zhang

et al. 2022

Vaccines

View full text Add to dashboard Cite

The bursa of Fabricius (BF) is a vital central humoral immune organ unique to birds. The bioactive peptide BP7 derived from bursa is reported to promote the vaccine immune response and antibody production. However, the regulatory effect on antigen presentation and B cell differentiation has been infrequently reported. In this paper, chicken macrophage HD11 cells were used for the cell model, and the cellular molecular expressions were determined by the fluorescent quantitative PCR (qPCR) after BP7 treatment. Then, the miRNA expression profile was analyzed by high-throughput sequencing. In addition, BALB/C mice were used as the animal model to detect B cell subtype with flow cytometry (FCM). The results showed that the expressions of four immune active molecules, IL-1β, IL-6, iNOS, and IFN-α, in HD11 cells were significantly increased with 100 ng/mL BP7 treatment. Compared with the control group, there were 58 up-regulated and 61 down-regulated miRNAs in HD11 cells with BP7 treatment. The gene ontology (GO) function analysis found that BP7 mainly affected the various biological processes, molecular function, and MHC protein complex. Pathway analysis showed that 100 ng/mL BP7 stimulated various physiological metabolic pathways and signal transduction pathways, including the intestinal immune network producing IgA in HD11 cells. Furthermore, it was found that BP7 in vitro stimulated B cell populations, as well as plasma cells in spleen cells from the immunized mice. Additionally, B cell activation subpopulations were increased in mice immunized with the AIV vaccine and BP7. These results proved that BP7 stimulated various differentially expressed genes in chicken macrophage HD11 cells, and induced B cell differentiation in the immunized mice, which suggested that BP7 might participate in the antigen presentation process, thereby promoting the differentiation of B cells. These results provide an important basis for the mechanism of bursal-derived peptide on B cell development, and offer the experimental basis for the development of adjuvants.

show abstract

microRNA, a Subtle Indicator of Human Cytomegalovirus against Host Immune Cells

Cited by 4 publications

References 132 publications

Clinical Differences between Posner-Schlossman Syndrome Patients with Intermittent Intraocular Pressure Elevation and Glaucomatous Damage

Clinical Differences between Posner-Schlossman Syndrome Patients with Intermittent Intraocular Pressure Elevation and Glaucomatous Damage

Human cytomegalovirus microRNAs: strategies for immune evasion and viral latency

Bursal-Derived BP7 Induces the miRNA Molecular Basis of Chicken Macrophages and Promotes the Differentiation of B Cells

Contact Info

Product

Resources

About