MicroRNA-98 interferes with thrombospondin 1 expression in peripheral B cells of patients with asthma

Chen, Liming; Xu, Jianfeng; Chu, Xiaoxia; Ju, Chenghua

doi:10.1042/bsr20170149

Cited by 14 publications

(9 citation statements)

References 23 publications

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“…All of these miRNAs have been shown to play roles in asthmatic inflammation. It was demonstrated that increased miR-98 in peripheral blood may contribute to pathogenesis of asthma by lowering the frequency of immune regulatory B cells [12] . There is some evidence to suggest that Let-7 has a pro-inflammatory role in asthma, primarily by promoting type 2 inflammation [13,14] .…”

Section: Association Of Mirna Expression Patterns With Mild Non-eosimentioning

confidence: 99%

Plasma microRNA profiles identify distinct clinical phenotypes in human asthmatics

Zhang¹,

Laryea²,

Panganiban³

et al. 2018

JTGG

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Aim: Asthma is a chronic inflammatory syndrome that is characterized by heterogeneous disease pathogeneses that produce distinct subtypes. There is a great need to develop biomarkers to distinguish these subtypes and help guide specific therapy and better predict outcomes, particularly in severe asthma where a number of targeted therapeutics are now available. We sought to determine whether expression of asthma-specific microRNAs (miRNAs) could distinguish phenotypic differences in a diverse cohort of asthmatic subjects that spanned a range of disease severity. Methods: RNA was isolated from peripheral blood from human subjects. Expression of 39 miRNAs was then screened. Sample cycle threshold values were normalized. Normally distributed data were analyzed and hierarchical cluster was performed. Results: Peripheral blood was obtained from 62 adults. We identified four clusters of asthmatics defined by 5 distinct miRNA expression patterns. Cluster 1 was associated with mild asthma, low inhaled corticosteroid use, and low eosinophil levels. Cluster 3 and 4 consisted primarily of severe asthmatics with poor asthma control, and Cluster 3 was specifically associated with high IgE, high blood eosinophil levels, and racial disparity (higher proportion of Black subjects). Cluster 2 was comprised predominantly of mild-moderate asthmatics that had higher blood eosinophils than Cluster 1. Conclusion: These results indicate the miRNA expression profiling can be useful to identify distinct asthma phenotypes, and have potential to better understand disease pathogenesis and help guide therapy.

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Section: Association Of Mirna Expression Patterns With Mild Non-eosimentioning

confidence: 99%

Plasma microRNA profiles identify distinct clinical phenotypes in human asthmatics

Zhang¹,

Laryea²,

Panganiban³

et al. 2018

JTGG

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“…MiRNAs have been linked to TGFβ-signaling pathway in a variety of diseases, usually in studies which only examined few miRNAs and genes within the signaling pathway. For instance, miR-181a has been shown to have its expression altered by TGFβ [ 24 , 25 ], miR-494 and miR-126-5p have been linked to BMP4 in the regulation of angiogenesis [ 26 ]; miR-98 with TBSP1 expression in asthma [ 27 ]; miR-590-5p with downregulation of TGFβ signaling in cardiosphere-derived stem cells [ 28 ]; the miR-17-92 cluster with cell proliferation in mouse mesenchymal cells [ 29 ]; and miR-140-5p with tumor growth [ 30 ]. MiRNAs have also been linked with various disease processes in CRC.…”

Section: Introductionmentioning

confidence: 99%

The TGFβ-signaling pathway and colorectal cancer: associations between dysregulated genes and miRNAs

et al. 2018

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BackgroundThe TGFβ-signaling pathway plays an important role in the pathogenesis of colorectal cancer (CRC). Loss of function of several genes within this pathway, such as bone morphogenetic proteins (BMPs) have been seen as key events in CRC progression.MethodsIn this study we comprehensively evaluate differential gene expression (RNASeq) of 81 genes in the TGFβ-signaling pathway and evaluate how dysregulated genes are associated with miRNA expression (Agilent Human miRNA Microarray V19.0). We utilize paired carcinoma and normal tissue from 217 CRC cases. We evaluate the associations between differentially expressed genes and miRNAs and sex, age, disease stage, and survival months.ResultsThirteen genes were significantly downregulated and 14 were significantly upregulated after considering fold change (FC) of > 1.50 or < 0.67 and multiple comparison adjustment. Bone morphogenetic protein genes BMP5, BMP6, and BMP2 and growth differentiation factor GDF7 were downregulated. BMP4, BMP7, INHBA (Inhibin beta A), TGFBR1, TGFB2, TGIF1, TGIF2, and TFDP1 were upregulated. In general, genes with the greatest dysregulation, such as BMP5 (FC 0.17, BMP6 (FC 0.25), BMP2 (FC 0.32), CDKN2B (FC 0.32), MYC (FC 3.70), BMP7 (FC 4.17), and INHBA (FC 9.34) showed dysregulation in the majority of the population (84.3, 77.4, 81.1, 80.2, 82.0, 51.2, and 75.1% respectively). Four genes, TGFBR2, ID4, ID1, and PITX2, were un-associated or slightly upregulated in microsatellite-stable (MSS) tumors while downregulated in microsatellite-unstable (MSI) tumors. Eight dysregulated genes were associated with miRNA differential expression. E2F5 and THBS1 were associated with one or two miRNAs; RBL1, TGFBR1, TGIF2, and INHBA were associated with seven or more miRNAs with multiple seed-region matches. Evaluation of the joint effects of mRNA:miRNA identified interactions that were stronger in more advanced disease stages and varied by survival months.ConclusionThese data support an interaction between miRNAs and genes in the TGFβ-signaling pathway in association with CRC risk. These interactions are associated with unique clinical characteristics that may provide targets for further investigations.Electronic supplementary materialThe online version of this article (10.1186/s12967-018-1566-8) contains supplementary material, which is available to authorized users.

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“…miR-147 also reduces the production of inflammatory proteins induced by TLR2, TLR3, and TLR4-mediation [27]. miRNA-98 has been implicated to be involved in asthma by modulating peripheral B cells which is an important immune regulatory cell, via interfering the expression of thrombospondin 1 [28]. miR-10a mediates airway hyperresponsiveness by suppresses cell proliferation of airway smooth muscle, via targeting BDNF in asthmatic rats [29].…”

Section: Discussionmentioning

confidence: 99%

Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede

Tang

Fang

et al. 2020

BioMed Research International

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Asthma is a common respiratory disease with inflammation in the lungs. Exosomes and microRNAs (miRNAs) play crucial role in inflammation, whereas the role of exosomal miRNA in asthma remains unknown. Here, we aimed to identify the key exosomal miRNAs and their underlying mechanisms involved in scorpio and centipede (SC) treatment in asthma. Eighteen mice were randomly divided into three groups: control group, asthma group, and SC treatment group. Effect of SC was assessed by hematoxylin-eosin staining and real-time PCR. Exosomes from asthma and SC treatment groups were analyzed by small RNA-seq. Results revealed SC significantly alleviated the pathogenesis of asthma and suppressed the release of inflammatory cytokines. A total of 328 exosomal miRNAs were differentially expressed between the exosomes from asthma and SC-treated mice, including 118 up- and 210 downregulated in SC-treated mice. The altered exosomal miRNAs were primarily involved in the function of transcription, apoptotic process, and cell adhesion; and pathway of calcium, Wnt, and MAPK signaling. Real-time PCR verified exosomal miR-147 was downregulated, while miR-98-5p and miR-10a-5p were upregulated in SC-treated mice compared to asthma mice. Moreover, the target genes of miR-147-3p, miR-98-5p, and miR-10a-5p were mainly enriched in Wnt and MAPK inflammatory signaling. miR-10a-5p promoted the proliferation of mouse lung epithelial cells and downregulated the expression of Nfat5 and Map2k6. These data suggest SC-induced exosomal miRNAs might mediate the inflammatory signaling and might be involved in the SC treatment in asthma. The exosomal miRNAs might be promising candidates for the treatment of asthma.

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MicroRNA-98 interferes with thrombospondin 1 expression in peripheral B cells of patients with asthma

Cited by 14 publications

References 23 publications

Plasma microRNA profiles identify distinct clinical phenotypes in human asthmatics

Plasma microRNA profiles identify distinct clinical phenotypes in human asthmatics

The TGFβ-signaling pathway and colorectal cancer: associations between dysregulated genes and miRNAs

Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede

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