microRNA-944 overexpression is a biomarker for poor prognosis of advanced cervical cancer

Park, Sunyoung; Kim, Jungho; Eom, Kiyoon; Oh, Seoung Wug; Kim, Sung-Hyun; Kim, Geehyuk; Ahn, Sung Gwe; Park, Kwang Hwa; Chung, Dawn; Lee, Hyeyoung

doi:10.1186/s12885-019-5620-6

Cited by 45 publications

(40 citation statements)

References 36 publications

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“…21,33 Numerous studies have further reported on the dual functions of miRs in carcinogenesis. [34][35][36][37] For example, miR-944 and miR-150-5p may function as oncogenes to exacerbate cervical carcinogenesis. 35,37 Inversely, miR-29a and miR-144-3p may act as tumor suppressors to inhibit invasion and metastasis of cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

miR‐587 promotes cervical cancer by repressing interferon regulatory factor 6

Ren

Dong

et al. 2020

The Journal of Gene Medicine

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Background: Interferon regulatory factor 6 (IRF6) exhibits tumor-suppressive functions in several cancer types. In the present study, the antitumor properties and related pathway mechanism of IRF6 were investigated in cervical cancer. Methods: Forty-one pairs of cervical cancer specimens and para-carcinoma tissues were collected to evaluate IRF6 expression using immunohistochemical staining and miR-587. The effects of miR-587 and IRF6 on cervical cancer cell growth were explored by MTT assays and in a HeLa tumor xenograft mouse model. The migration and invasion of cervical cancer cells were monitored using transwell assays. Results: IRF6 expression in cervical cancer specimens and cell lines was significantly reduced compared to that in the corresponding control group. In addition, IRF6 expression was negatively correlated with miR-587 in cervical cancer tissues. Bioinformatics algorithms and luciferase assays revealed that IRF6 is a potential target of miR-587, and miR-587 mimic transfection led to a significant repression of IRF6 protein levels in cervical cancer cells. We also discovered that the antineoplastic properties of IRF6 could be reversed by overexpressing miR-587 in cervical cancer cells. The up-regulation of miR-587 was correlated with poor overall survival in cervical cancer. In an in vivo experiment, miR-587 silencing induced HeLa tumor growth inhibition, which was associated with the up-regulation of IRF6 protein in the tumor. Conclusions: miR-587 post-transcriptionally represses IRF6 protein expression to abrogate the antineoplastic activity of IRF6. The miR-587/IRF6 signaling pathway plays a crucial role in the progression of cervical cancer and serves as a potential therapeutic target for the treatment of cervical cancer. K E Y W O R D S cervical cancer, interferon regulatory factor 6, miR-587, prognosis 1 | INTRODUCTION Cervical cancer is one of the most common gynecologic malignant neoplasms and ranks fourth for both incidence and mortality in females. 1 According to an epidemiological investigation, cervical cancer accounted for approximately 6.6% of the total incidents and 7.5% of the total cancer deaths among females in 2018. 1 In general, persistent human papillomavirus infection can lead to intraepithelial lesions of the cervix uteri, which is an inevitable cancerous process of cervical cancer. 2,3 The mechanisms of cervical carcinogenesis are complicated. Different signaling pathways, such as nuclear factorkappa B, mammalian target of rapamycin, phosphoinositide 3-kinase Yuefang Ren and Jie Dong contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

miR‐587 promotes cervical cancer by repressing interferon regulatory factor 6

Ren

Dong

et al. 2020

The Journal of Gene Medicine

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“…miR-944 was significantly higher expressed in cervical cancer tissues compared to controls, as well as in HPV-positive samples compared to those that were HPV-negative [142]. Its expression was associated with advanced FIGO stage, bulky tumor size, lymph node metastasis, and lower survival rates [142]. Considering all of the above, miR-944 could potentially serve as a prognostic biomarker [142].…”

Section: Mirnas In Cervical Cancermentioning

confidence: 99%

“…Although many studies stated that it functions as an oncogene in various malignancies, among them being cervical cancer, by stimulating cell migration, invasion and proliferation [140,141], there are some that described tumor suppressor properties in cancers located in the colon, stomach, and breast [159][160][161]. The hypothesis that it might have oncogenic functions was recently studied using five types of cell lines and 116 cervical tissues [142]. miR-944 was significantly higher expressed in cervical cancer tissues compared to controls, as well as in HPV-positive samples compared to those that were HPV-negative [142].…”

Section: Mirnas In Cervical Cancermentioning

confidence: 99%

“…The hypothesis that it might have oncogenic functions was recently studied using five types of cell lines and 116 cervical tissues [142]. miR-944 was significantly higher expressed in cervical cancer tissues compared to controls, as well as in HPV-positive samples compared to those that were HPV-negative [142]. Its expression was associated with advanced FIGO stage, bulky tumor size, lymph node metastasis, and lower survival rates [142].…”

Section: Mirnas In Cervical Cancermentioning

confidence: 99%

“…Its expression was associated with advanced FIGO stage, bulky tumor size, lymph node metastasis, and lower survival rates [142]. Considering all of the above, miR-944 could potentially serve as a prognostic biomarker [142].…”

Section: Mirnas In Cervical Cancermentioning

confidence: 99%

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miRNAs as Biomarkers in Disease: Latest Findings Regarding Their Role in Diagnosis and Prognosis

Condrat

Thompson

Barbu

et al. 2020

Cells

843

583

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MicroRNAs (miRNAs) represent a class of small, non-coding RNAs with the main roles of regulating mRNA through its degradation and adjusting protein levels. In recent years, extraordinary progress has been made in terms of identifying the origin and exact functions of miRNA, focusing on their potential use in both the research and the clinical field. This review aims at improving the current understanding of these molecules and their applicability in the medical field. A thorough analysis of the literature consulting resources available in online databases such as NCBI, PubMed, Medline, ScienceDirect, and UpToDate was performed. There is promising evidence that in spite of the lack of standardized protocols regarding the use of miRNAs in current clinical practice, they constitute a reliable tool for future use. These molecules meet most of the required criteria for being an ideal biomarker, such as accessibility, high specificity, and sensitivity. Despite present limitations, miRNAs as biomarkers for various conditions remain an impressive research field. As current techniques evolve, we anticipate that miRNAs will become a routine approach in the development of personalized patient profiles, thus permitting more specific therapeutic interventions.Biogenesis of miRNA begins in the nucleus, where the transcription of its precursor, primary miRNA or pri-miRNA takes place under the influence of RNA polymerases II and III [11,12]. The resulting molecule is a hairpin-like structure, which contains a loop at one end [11]. This primordial mi-RNA precursor that is usually made up of hundreds of nucleotides is then processed consecutively by two RNase III enzymes [13][14][15]. The first enzyme to act upon the pri-miRNA, which still resides in the nucleus, is called Drosha or DCGR8, and turns it into a new hairpin-like structure of approximately 70 nucleotides, the Precursor-miRNA or pre-miRNA. The latter is then transported to the cytoplasm, with the help of Exportin-5, where it is cleaved again by the Ago2/Dicer complex leading to the short, mature miRNA double strands [16].Further on, one of the strands, usually known as the guide strand, will be integrated into the RNA-induced silencing complex (RISC), while the other one, known as the passenger strand, is going to be degraded, even though in some occasions it has been found to be also functional [17]. In most cases, the strand that contains the less stable 5 end or a uracil at the beginning is more likely to be selected as the guide strand [18][19][20]. In those situations, where the passenger strand is not degraded and both get incorporated into the miRISC complex, the mature miRNA in the guide strand will be the dominant one [21,22].The main role of miRNA in the human body is gene regulation [23] by mediating the degradation of mRNA and also by regulating transcription and translation through canonical and non-canonical mechanisms [4]. The canonical mechanism means that the miRISC complex containing the miRNA guide strand is exerting its action by binding to the targ...

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Advances in exosome biomarkers for cervical cancer

Ran

et al. 2022

Cancer Medicine

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Cervical cancer (CC) ranks as the fourth most frequently diagnosed malignancy in females worldwide. Exosomes are a subclass of extracellular vesicles released by nearly all types of cells that act as cargo transport vehicles, carrying proteins, and genetic material (such as miRNAs, long noncoding RNAs, and mRNAs) derived from their parent cells may affect receiving cells and thus have emerged as key players in several biological processes, including inflammatory pathways. In this review, we concentrated on the findings of exosome investigations in CC, particularly their components. They direct the actions of CC cells by inducing surface molecules associated with various biological pathways. We summarized the current knowledge of exosomal RNAs and proteins from CC cells and discussed the feasibility of exosomes as potential biomarkers for CC. We suggest that cancer-derived exosomes promote metastasis in CC by supporting EMT, controlling the proliferation, invasion, or migration of cancer cells, as well as influencing immune escape and aiding angiogenesis. Overall, cancer-derived exosomes are critical in the progression of CC, and further studies are necessary to advance our understanding of the clinical value of exosomes in CC.

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microRNA-944 overexpression is a biomarker for poor prognosis of advanced cervical cancer

Cited by 45 publications

References 36 publications

miR‐587 promotes cervical cancer by repressing interferon regulatory factor 6

miR‐587 promotes cervical cancer by repressing interferon regulatory factor 6

miRNAs as Biomarkers in Disease: Latest Findings Regarding Their Role in Diagnosis and Prognosis

Advances in exosome biomarkers for cervical cancer

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