MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2

Abstract

Colorectal cancer (CRC) is a global public health issue with increasing prevalence. MicroRNA-934 (miR-934) is a kind of non-coding RNA involved in the regulation of diverse cancers. Though previous researches have revealed part of association between miR-934 and CRC, the role of miR-934 in CRC pathogenesis has not been completely explored yet. In this study, we aim to investigate the effect of miR-934 on cell proliferation, migration, invasion and angiogenesis in CRC. Accordingly, miR-934 was found to be over-expressed in SW480 and HCT116 cells, two typical CRC cell lines. Meanwhile, miR-934 knockdown significantly inhibited cell proliferation and induced cell cycle arrest in SW480 and HCT116 cells. It was further validated that miR-934 knockdown displayed an inhibitory effect on cell migration and invasion in SW480 and HCT116 cells. Additionally, miR-934 deficiency markedly decreased VEGF expression in SW480 and HCT116 cells and suppressed capability of CRC cells to promote tube formation in vascular endothelial cells, which suggests the pro-angiogenesis role of miR-934 in vitro. Dual luciferase reporter assay further showed that miR-934 directly bound to B-cell translocation gene 2 (BTG2). BTG2 knockdown reversed the inhibitory effect of miR-934 silencing on cell proliferation, migration, invasion, and angiogenesis in SW480 and HCT116 cells. In summary, this study suggests that miR-934 facilitates CRC progression by targeting BTG2, and further highlights the role of miR-934 in pathogenesis of CRC.

A Phase II Exploratory Study to Identify Biomarkers Predictive of Clinical Response to Regorafenib in Patients with Metastatic Colorectal Cancer Who Have Failed First-Line Therapy

“…Interestingly, this study also shed light on the heightened expression of three miRNAs (miR-365a-5p, miR-6510-3p, and miR-934) and a heightened incidence of mutations affecting amino acids 12 or 13 in KRAS within resistant lesions, providing potential candidates for future research into predictors of regorafenib treatment response. Two of the miRNAs (miR-365a-5p and miR-934) have already been described as targeting genes involved in cellular proliferation and cell signaling pathways [ 48 , 49 ]. Their higher level of expression in resistant lesions could affect downstream targets of regorafenib inhibition and cancel its action.…”

Elevated BTG2 improves the radiosensitivity of non‐small cell lung cancer (NSCLC) through apoptosis

“…BTG2 is expressed at low levels in multiple cancers and might therefore act as a tumor suppressor. [25][26][27] BTG2 plays an important role in cell proliferation, migration, invasion and drug resistance. 28,29 Little research has been performed on the biological behavior of BTG2 in response to IR stress.…”

“…The B cell translocation gene 2 (BTG2) is one of the BTG/TOB family genes. BTG2 is expressed at low levels in multiple cancers and might therefore act as a tumor suppressor 25–27 . BTG2 plays an important role in cell proliferation, migration, invasion and drug resistance 28,29 .…”

The ATF2/miR-3913-5p/CREB5 axis is involved in the cell proliferation and metastasis of colorectal cancer

“…To date, accumulating studies have indicated the certain miRNAs involved in the tumor progression of CRC. For instance, miR-934 facilitates CRC cell growth, migration, invasion and angiogenesis by targeting BTG2 10 . miR-576-5p participates in the suppression of CRC progression induced by hsa_circ_0001666 11 .…”