MicroRNA-92b regulates the development of intermediate cortical progenitors in embryonic mouse brain

Nowakowski, Tomasz J.; Fotaki, Vassiliki; Pollock, Andrew; Sun, Tao; Pratt, Thomas; Price, David J.

doi:10.1073/pnas.1219385110

Cited by 90 publications

(77 citation statements)

References 31 publications

(48 reference statements)

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“…Consistent with neuro-ectodermal priming, derived hIPSC-Ts also expressed higher levels of miRNAs associated with neuronal progenitors (e.g. members of miR-25 family) (Nowakowski et al, 2013). …”

Section: Resultsmentioning

confidence: 92%

Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency

Cacchiarelli

Trapnell

Ziller

et al. 2015

Cell

209

284

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Summary Induced pluripotency is a promising avenue for disease modeling and therapy, but the molecular principles underlying this process, particularly in human cells, remain poorly understood due to donor-to-donor variability and intercellular heterogeneity. Here we constructed and characterized a clonal, inducible human reprogramming system that provides a reliable source of cells at any stage of the process. This system enabled integrative transcriptional and epigenomic analysis across the human reprogramming timeline at high resolution. We observed distinct waves of gene network activation, including the ordered reactivation of broad developmental regulators followed by early embryonic patterning genes and culminating in the emergence of a signature reminiscent of pre-implantation stages. Moreover, complementary functional analyses allowed us to identify and validate novel regulators of the reprogramming process. Altogether, this study sheds light on the molecular underpinnings of induced pluripotency in human cells and provides a robust cell platform for further studies.

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Section: Resultsmentioning

confidence: 92%

Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency

Cacchiarelli

Trapnell

Ziller

et al. 2015

Cell

209

284

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“…Recent research has identified the role of key miRNAs in the developing mouse cortex. For instance, miR-92 maintains proper populations of cortical radial glial cells and intermediate progenitor cells through repression of PTEN and Tbr2 proteins, which in turn modulates subsequent neuronal production (Bian et al, 2013;Nowakowski et al, 2013). Interestingly, miR-92 has been shown to play a key role in maintaining asymmetric self-renewing divisions in the mouse cortex via Tis21 downregulation (Fei et al, 2014).…”

Section: A Novel Regulatory Architecture Within the Primate Germinal mentioning

confidence: 99%

The Outer Subventricular Zone and Primate-Specific Cortical Complexification

Dehay

Kennedy

Kosik

2015

Neuron

273

302

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Evolutionary expansion and complexification of the primate cerebral cortex are largely linked to the emergence of the outer subventricular zone (OSVZ), a uniquely structured germinal zone that generates the expanded primate supragranular layers. The primate OSVZ departs from rodent germinal zones in that it includes a higher diversity of precursor types, inter-related in bidirectional non-hierarchical lineages. In addition, primate-specific regulatory mechanisms are operating in primate cortical precursors via the occurrence of novel miRNAs. Here, we propose that the origin and evolutionary importance of the OSVZ is related to genetic changes in multiple regulatory loops and that cell-cycle regulation is a favored target for evolutionary adaptation of the cortex.

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“…MiR92 and miR92b are potential inhibitors of Eomes mRNA translation [73] and are highly expressed in aRG committed to generating BPs [35 ]. Moreover, miR92 restricts the expression of the prodifferentiative gene Tis21 via its 3 0 UTR, thereby ensuring maintenance of asymmetric self-renewing aRG divisions during mouse cortical development, whereas mice lacking the Tis21 3 0 UTR develop a microcephalic neocortex [74].…”

Section: Micrornasmentioning

confidence: 99%