MicroRNA‑769‑3p inhibits tumor progression in glioma by suppressing ZEB2 and inhibiting the Wnt/β‑catenin signaling pathway

Abstract

Accumulating evidence suggests the crucial role of microRNAs (miRNAs) in human cancers. The present study aimed to investigate the clinical and functional roles of miR-769-3p in glioma, as well as the underlying molecular mechanisms. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine the expression levels of miR-769-3p in glioma tissues and cells. Receiver operating characteristic (ROC) curve analysis was applied to calculate the diagnostic value of miR-769-3p. The 5-year survival rate of patients was calculated using Kaplan-Meier analysis and Cox regression analysis. Cell experiments were used to investigate the functional role of miR-769-3p in glioma. The gene target of miR-769-3p was predicted by TargetScan. Changes in the levels of Wnt signaling-related proteins were measured by western blotting. miR-769-3p was significantly downregulated in glioma tissues and serum, as well as in glioma cell lines (P<0.001). miR-769-3p expression was significantly associated with the World Health Organization grade and Karnofsky performance score. The ROC curves demonstrated that serum miR-769-3p level reliably distinguished patients with glioma from healthy individuals. High tissue miR-769-3p expression predicted poor overall survival in patients with glioma (log-rank P=0.001) and was identified as an independent prognostic factor. In addition, zinc finger E-box binding homeobox 2 (ZEB2) was demonstrated to be a direct target of miR-769-3p in glioma cells using a luciferase assay. miR-769-3p upregulation suppressed the activity of the Wnt/β-catenin signaling pathway in glioma cells. In conclusion, miR-769-3p may serve as a diagnostic and prognostic biomarker in patients with glioma and target ZEB2 to inhibit tumor progression via the Wnt/β-catenin signaling pathway. miR-769-3p may be a novel therapeutic target for the treatment of glioma.

Prognostic Potential of MicroRNAs in Glioma Patients: A Meta-Analysis

“…The 11 studies selected for the purpose of this study 20 21 22 23 24 25 26 27 28 29 30 as seen in Table 3 underwent random effect model for the overall survival analysis. This was due to significant heterogeneity being observed in the selected studies ( p < 0.05, I 2 = 85.9%).…”

CircRNA circYY1 (hsa_circ_0101187) Modulates Cell Glycolysis and Malignancy Through Regulating YY1 Expression by Sponging miR-769-3p in Breast Cancer

“…Moreover, Wang et al discovered that miR-769-3p blocked the Wnt/β-catenin pathway via targeting ZEB2 in glioma cells, thereby repressing tumor growth. 27 However, Ju et al revealed that miR-769-3p was a potential predictor of lung metastasis, which might be related to the specificity of cells and tissues. 28 …”

LncRNA ADAMTS9-AS1 knockdown suppresses cell proliferation and migration in glioma via down-regulating Wnt/β-catenin signaling pathway

“…Our data further elucidated the effects of ADAMTS9-AS1 on the molecular mechanisms underlying ADAMTS9-AS1 knockdown suppressing glioma cell proliferation, migration and invasion. Typically, activation of the Wnt/β-catenin signaling pathway is considered to be one of the most important molecular pathways involved in the development of various human cancers, including glioma (27,28). Moreover, inhibition of Wnt/β-catenin signaling can block the process of epithelial-mesenchymal transition (EMT) during the cancer progression (29).…”