MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling

Hayakawa, Kunihiro; Kawasaki, Mikiko; Hirai, Takuya; Yoshida, Yuko; Tsushima, Hirofumi; Fujishiro, Midori; Ikeda, Keigo; Morimoto, Shinji; Takamori, Kenji; Sekigawa, Iwao

doi:10.3390/ijms20040809

Cited by 42 publications

(38 citation statements)

References 34 publications

(34 reference statements)

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“…The most highly up-regulated candidates in COVID-19 patients were miR-31-5p (associated with in ammatory disorders [18][19][20] ) (Figure 1a), miR-3125 and miR-4742-3p, while the most down-regulated were miR-1275 (Figure 1a), miR-3617-5p and miR-500b-3p. The most statistically signi cant change was seen in miR-766-3p (Figure 1a), a known anti-in ammatory miRNA 21 . Unsupervised analysis of variance using principal components analysis (PCA) involving the 55 DE miRNAs showed tight clustering of patient groups (Figure 1b).…”

Section: Resultsmentioning

confidence: 99%

“…Other patient cytokine data is shown in Supplementary Table 2. One signi cantly reduced miRNA, miR-766-3p, has been shown to reduce IL-6 expression in a dose-dependent manner 21 , while another, miR--4662a-5p (Figure 1c), is predicted to target IL-6 mRNA (TargetScan, release 7.2) 22 . The miRNAs may play a role in the hyperin ammatory state commonly seen in patients with COVID-19 23 .…”

Section: Resultsmentioning

confidence: 99%

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Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection

Farr

Rootes

Rowntree

et al. 2021

Preprint

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The host response to SARS-CoV-2 infection provide insights into both viral pathogenesis and patient management. The host-encoded microRNA (miRNA) response to SARS-CoV-2 infection, however, remains poorly defined. Here we profiled circulating miRNAs from ten COVID-19 patients sampled longitudinally and ten age and gender matched healthy donors. We observed 55 miRNAs that were altered in COVID-19 patients during early-stage disease, with the inflammatory miR-31-5p the most strongly upregulated. Supervised machine learning analysis revealed that a three-miRNA signature (miR-423-5p, miR-23a-3p and miR-195-5p) independently classified COVID-19 cases with an accuracy of 99.9%. In a ferret COVID-19 model, the three-miRNA signature again detected SARS-CoV-2 infection with 99.7% accuracy, and distinguished SARS-CoV-2 infection from influenza A (H1N1) infection and healthy controls with 95% accuracy. Distinct miRNA profiles were also observed in COVID-19 patients requiring oxygenation. This study demonstrates that SARS-CoV-2 infection induces a robust host miRNA response that could improve COVID-19 detection and patient management

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection

Farr

Rootes

Rowntree

et al. 2021

Preprint

View full text Add to dashboard Cite

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“… hsa-miR-361 Overexpression in cutaneous leishmaniosis lesions, impairs epidermal barrier function by filaggrin-2 repression [ 96 ]. hsa-miR-766 Indirectly inhibits of NF-κB signaling causing anti-inflammatory response [ 97 ]. hsa-miR-769 Expression is significantly correlated with the presence of pronounced coronary atherosclerosis [ 98 ], inhibits colorectal cancer cell proliferation and invasion by targeting HEY1 (downstream effector of NOTCH signaling pathway) [ 99 ], negatively correlated with EGFR expression [ 100 ].…”

Section: Abdominal Aorta Aneurysm and Control Groups Constructionmentioning

confidence: 99%

Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm

Zalewski

Ruszel

Stępniewski

et al. 2020

JCM

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Abdominal artery aneurysm (AAA) refers to abdominal aortic dilatation of 3 cm or greater. AAA is frequently underdiagnosed due to often asymptomatic character of the disease, leading to elevated mortality due to aneurysm rupture. MiRNA constitute a pool of small RNAs controlling gene expression and is involved in many pathologic conditions in human. Targeted panel detecting altered expression of miRNA and genes involved in AAA would improve early diagnosis of this disease. In the presented study, we selected and analyzed miRNA and gene expression signatures in AAA patients. Next, generation sequencing was applied to obtain miRNA and gene-wide expression profiles from peripheral blood mononuclear cells in individuals with AAA and healthy controls. Differential expression analysis was performed using DESeq2 and uninformative variable elimination by partial least squares (UVE-PLS) methods. A total of 31 miRNAs and 51 genes were selected as the most promising biomarkers of AAA. Receiver operating characteristics (ROC) analysis showed good diagnostic ability of proposed biomarkers. Genes regulated by selected miRNAs were determined in silico and associated with functional terms closely related to cardiovascular and neurological diseases. Proposed biomarkers may be used for new diagnostic and therapeutic approaches in management of AAA. The findings will also contribute to the pool of knowledge about miRNA-dependent regulatory mechanisms involved in pathology of that disease.

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“…Previous studies have reported differences in the expression levels of miRNAs in the peripheral blood of patients with stroke compared with healthy individuals ( 33 , 34 ). In addition, an increasing number of studies have demonstrated that certain miRNAs may exert important cerebroprotective effects against IRI ( 35 , 36 ). miR-766-5p has been reported to exert anti-inflammatory effects on human rheumatoid arthritis fibroblast-like synoviocyte MH7A cells ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA TTTY15 alleviates ischemia/reperfusion‑induced inflammation and apoptosis of PC12 cells by targeting miR‑766‑5p

Hao

Chen²

2021

Exp Ther Med

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The pathogenesis of ischemic stroke is extremely complex and has a significant impact on the quality of life of the patients. Accumulating studies have reported that long non-coding RNAs (lncRNAs) may be associated with the progression of ischemic stroke. However, the role and underlying mechanism of action of the lncRNA testis-specific transcript Y-linked 15 (TTTY15) in ischemic stroke remains unknown. The present study analyzed the expression levels of TTTY15 in PC12 cells injured by oxygen-glucose deprivation/reperfusion (OGD/R). The effects of the knockdown of TTTY15 expression on the levels of the inflammatory cytokines TNF-α, IL-1β, IL-18 and IL-10, cell apoptosis and the expression levels of the apoptosis-associated proteins Bcl-2, Bax, cleaved caspase-3, caspase-3, cleaved caspase-9 and caspase-9, were subsequently analyzed in OGD/R-treated PC12 cells using ELISA, flow cytometry and western blotting, respectively. In addition, the downstream target gene of TTTY15 was verified using a dual luciferase reporter assay. The effects of TTTY15 on the inflammation and apoptosis of PC12 cells treated with OGD/R were determined by targeting miR-766-5p. The results of the present study revealed that TTTY15 expression was upregulated in OGD/R-treated PC12 cells. The knockdown of TTTY15 significantly decreased the concentrations of the proinflammatory factors TNF-α, IL-1β and IL-18, while it increased the concentration of the anti-inflammatory cytokine IL-10 in OGD/R-treated PC12 cells. Apoptosis was also suppressed following gene silencing of TTTY15. Subsequently, miR-766-5p was identified as a target gene of TTTY15 using a dual luciferase reporter assay and the expression levels of TTTY15 and miR-766-5p were found to be negatively correlated. The overexpression of miR-766-5p alleviated the stimulatory effect of TTTY15 overexpression on the inflammation and apoptosis of PC12 cells treated with OGD/R. Therefore, the present study revealed that TTTY15 knockdown improved the OGD/R-induced injury of PC12 cells by upregulating miR-766-5p expression.

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MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling

Cited by 42 publications

References 34 publications

Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection

Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection

Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm

Knockdown of lncRNA TTTY15 alleviates ischemia/reperfusion‑induced inflammation and apoptosis of PC12 cells by targeting miR‑766‑5p

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